Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof

A technology of compound microorganisms and compound microorganism inoculants, applied in the fields of agricultural fertilizers and biological control, can solve problems such as the inability of beneficial bacteria to colonize, and achieve the effects of good rhizosphere colonization ability, improved yield and quality, and stable efficacy.

Active Publication Date: 2016-08-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies also found that continuous cropping of Pseudostellariae Pseudostellariae led to significant changes in the soil environment, such as acidification, and Pseudostellariae Pseudostellaria continued to release a variety of secondary metabolites and self-toxic substances. Colonizes in the rhizosphere of

Method used

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  • Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof
  • Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof
  • Special composite microbial fertilizer for radix psuedostellariae, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and identification of indigenous antagonistic bacteria

[0034] Pseudomonas selective medium and Bacillus selective medium were used to separate Pseudomonas rhizosphere and Bacillus of Pseudomonas ginseng respectively, and inorganic culture medium (containing higher concentration of self-toxic substances from Pseudomonas The toxic substance-degrading bacteria are separated.

[0035] The single colonies randomly selected on the above-mentioned different media were used PDA plate confrontation method to evaluate their antagonistic effects on the specialized pathogenic bacteria (such as Fusarium oxysporum, Fusarium moniliforme, Talaromyces, etc.). The bacteria with strong antagonistic ability were identified by 16s rRNA gene or 16s-23s rRNA interval PCR identification for molecular identification. 16s rRNA gene PCR amplification upstream and downstream primers are: 27f (5'-AGAGTTTGATCCTGGCTCAG-3') and 1522r (5'-AAGGAGGTGAT CCAGCCGCA-3'); 16s-23s rRNA gene P...

Embodiment 2

[0045] Example 2 Preparation of special compound microbial fertilizer

[0046] The special compound microbial fertilizer of the present invention is made by fermenting the special compound microbial inoculum and organic materials together. Among them, the special compound microbial inoculum is made by mixing general beneficial bacteria liquid (EM compound bacterial liquid) and indigenous beneficial bacteria liquid (IM compound bacterial liquid) in a ratio of 4:6 by volume. The specific preparation method is as follows:

[0047] 2.1 Preparation of EM compound bacterial solution

[0048] Add 300g of brown sugar and 50mL of MS mother liquor to 18L of well water (tap water should be used for at least 2 days), then add 1L of activated commercially available EM liquid strain, and place it in a 37°C room for airtight fermentation for 3 weeks. From the next day, you can open the mouth of the closed container to release air and reduce pressure once every other day. When you open the mouth o...

Embodiment 3

[0061] Example 3 Evaluation of the effect of special compound microbial fertilizer on preventing and controlling continuous cropping obstacles of ginseng

[0062] First, use the prepared special compound microbial fertilizer as the base fertilizer to improve the continuous cropping soil of P. sylvestris, then prepare the ground, plant P. sylvestris, and irrigate the roots and topdress the special compound microbial liquid for fermented fertilizer from the seedling stage of P. sylvestris. Bacteria, compare the yield difference during the harvest period. The specific control measures are as follows:

[0063] 3.1 Preparation of microbial organic base fertilizer

[0064] Mix and mix organic materials such as bean cypress, rice bran, dried pig manure, fish meal, and brown sugar at a weight ratio of 2:1:1:1:0.03, and then add a special compound microbial liquid inoculant (1L / 10kg) to control the water content At 30%, mix well and then divide into sealed anaerobic fermentation at 37°C for...

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Abstract

The invention relates to a special composite microbial fertilizer for controlling successive cropping obstacles of radix psuedostellariae. The fertilizer is prepared by fermenting a special composite microbial inoculant and organic materials (bean pulp, rice bran, dried pig manure, fish meal and brown sugar in a weight ratio of 2:1:1:1:0.03). The composite microbial inoculant is prepared by mixing a universal beneficial bacterium solution and an aboriginal beneficial bacterium solution in a volume ratio of 4:6. The aboriginal beneficial bacterium comprises an aboriginal beneficial bacterium composite bacterium solution which is formed by independently fermenting Burkholderia, pseudomonad and Bacillus separated to the radix psuedostellariae rhizosphere soil, and mixing in a volume ratio of 4:1:1. The organic materials are subjected to special composite microbial inoculant anaerobic fermentation and oxygen-consuming fermentation to prepare the special composite microbial fertilizer. The long-term on-site verification proves that the microbial fertilizer has the advantages of reasonable formula, stable effects, favorable biocontrol effect and no pollution, has obvious effects on controlling succession cropping obstacles of radix psuedostellariae, is safe and ecological, and thus, has wide development and application prospects.

Description

Technical field [0001] The invention relates to a special compound microbial fertilizer for P. sylvestris and its preparation method and application, which can be used for preventing and curing the problem of continuous cropping of medicinal plant P. sylvestris, belonging to the technical field of agricultural fertilizers and biological control. Background technique [0002] Pseudostellaria heterophylla (Pseudostellaria heterophylla), also known as Tongshen and Haier's ginseng, is a perennial herb of the Caryophyllaceae family. It is used as medicine with its roots. It has the functions of invigorating qi and strengthening the spleen, promoting body fluid and nourishing the lung. However, there is a serious problem of continuous cropping obstacles in the planting process of P. sylvestris, which is also known as the problem of repeated cropping or replanting diseases. That is, planting P. sylvestris on the same plot for many years has caused the roots of P. sylvestris to not swell ...

Claims

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Application Information

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IPC IPC(8): C05F17/00C05G3/04A01C21/00C12N1/20C12N1/16C12R1/40C12R1/39C12R1/38C12R1/07C12R1/125C12R1/085C12R1/11C05G3/80
CPCA01C21/00C05F1/005C05F17/50C05G3/80C12N1/16C12N1/20C05F3/00C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 吴林坤林文雄陈军吴红淼林生
Owner FUJIAN AGRI & FORESTRY UNIV
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