Acetylated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
A kind of hollyhock, acetylation technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, food science, etc., can solve the problems of less active research, improve immune activity, realize sustainable application, good economy value effect
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Embodiment 1
[0031] The preparation method of the polysaccharide acetylation modification product of the stem and leaf of hollyhock comprises the following steps:
[0032] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock
[0033] Take 100 g of dry dregs of hollyhock stems and leaves extracted by ethanol reflux, add water 30 times the weight of the dregs, and extract by reflux in a water bath at 100°C for 3 times, each time for 1 h. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.
[0034] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock
[0035] Weigh 3 g of the crude...
Embodiment 2
[0038] Example 2 Determination of total sugar content and degree of substitution of polysaccharide acetylation modification products of hollyhock stems and leaves
[0039] 1. Determination of total sugar content and degree of acetylation substitution
[0040] With D-anhydrous glucose as the standard, the total sugar content of crude polysaccharides in the stems and leaves of hollyhock before and after the acetylation modification was determined by the phenol-sulfuric acid method; , titrate the unreacted base with hydrochloric acid, and calculate the acetyl group content (A%) and the degree of substitution (DS) according to the following formula.
[0041]
[0042]
[0043] In the formula: A (%) is the acetyl group content; V0 (mL) represents the volume of the sodium hydroxide solution consumed; C0 (mol / L) represents the concentration of the NaOH solution consumed; V1 (mL) represents the consumed The volume of the hydrochloric acid solution; C1 (mol / L) represents the conc...
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