Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Acetylated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

A kind of hollyhock, acetylation technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, food science, etc., can solve the problems of less active research, improve immune activity, realize sustainable application, good economy value effect

Inactive Publication Date: 2018-07-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign studies on polysaccharides from stems and leaves of hollyhock mainly focus on the analysis of physical and chemical properties and monosaccharides, and there are few studies on its activity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acetylated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Acetylated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof
  • Acetylated modified product of hollyhock stem and leaf polysaccharide with enhanced immune activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the polysaccharide acetylation modification product of the stem and leaf of hollyhock comprises the following steps:

[0032] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock

[0033] Take 100 g of dry dregs of hollyhock stems and leaves extracted by ethanol reflux, add water 30 times the weight of the dregs, and extract by reflux in a water bath at 100°C for 3 times, each time for 1 h. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.

[0034] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock

[0035] Weigh 3 g of the crude...

Embodiment 2

[0038] Example 2 Determination of total sugar content and degree of substitution of polysaccharide acetylation modification products of hollyhock stems and leaves

[0039] 1. Determination of total sugar content and degree of acetylation substitution

[0040] With D-anhydrous glucose as the standard, the total sugar content of crude polysaccharides in the stems and leaves of hollyhock before and after the acetylation modification was determined by the phenol-sulfuric acid method; , titrate the unreacted base with hydrochloric acid, and calculate the acetyl group content (A%) and the degree of substitution (DS) according to the following formula.

[0041]

[0042]

[0043] In the formula: A (%) is the acetyl group content; V0 (mL) represents the volume of the sodium hydroxide solution consumed; C0 (mol / L) represents the concentration of the NaOH solution consumed; V1 (mL) represents the consumed The volume of the hydrochloric acid solution; C1 (mol / L) represents the conc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses an abelmoschus manihot stem and leaf polysaccharide acetylation modification product with an immunocompetence improving function and a preparation method thereof. The abelmoschus manihot stem and leaf polysaccharide acetylation modification product contains 16.83% of an acetyl group and 82.50% of a polysaccharide, wherein the polysaccharide is prepared from mannose, glucose, galactose and arabinose according to the mole ratio of 0.004:1.49:0.008:0.011. The preparation method comprises the steps that an extraction and separation technology is preferably selected through a large quantity of experiments, a crude polysaccharide is obtained by adopting a water extraction and alcohol precipitation method, deproteinization is conducted, then purification is conducted by adopting DEAE-52 celluosic resin to prepare the abelmoschus manihot stem and leaf polysaccharide with the high purity, and finally modification is conducted by adopting an acetic anhydride method to obtain the abelmoschus manihot stem and leaf polysaccharide acetylation modification product. Accordingly, the waste abelmoschus manihot stem and leaf resources are fully utilized, the waste materials are changed into things of value, the abelmoschus manihot stem and leaf polysaccharide acetylation modification product capable of improving the immunocompetence is obtained, sustainable application of the traditional Chinese medicine resources can be achieved, and the very good economic value is achieved.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to an acetylated modified product of hollyhock stem and leaf polysaccharide with the ability to improve immunity and a preparation method thereof. Background technique [0002] Traditional Chinese medicine resources are a solid foundation for safeguarding national health and developing ethnic medicine. In recent years, the production area of ​​traditional Chinese medicine and natural medicinal biological resources has exceeded 2.40×10 6 hm 2 , the output of medicinal materials can reach 5.40×10 6 t, while the biomass of discarded plant roots and aboveground stems and leaves is as high as 1.1×10 7 ~1.6×10 7 t is 2 to 3 times the output of medicinal materials, causing serious waste of resources and environmental pollution. Therefore, in the process of industrialization of traditional Chinese medicine resources, improving the utilization value of medicinal biological resources, especially...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02C08B37/14C08B37/00A61K31/737A61P37/04A23L33/125
CPCA61K31/737C08B37/0003C08B37/0009C08B37/0036C08B37/0087
Inventor 江曙潘欣欣段金廒朱悦钱大玮严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products