A kind of method utilizing sphingomonas to split hepatitis C ns3 enzyme inhibitor medicine intermediate

A technology of sphingomonas and hepatitis C, which is applied in the field of splitting hepatitis C NS3 enzyme inhibitor drug intermediates by using sphingomonas, which can solve the problems of low split efficiency and large usage , to achieve the effect of high resolution activity, shortened enzyme dosage and low price

Active Publication Date: 2019-08-02
BEIJING UNIV OF CHEM TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there is no report on the use of microbial whole-cell method for splitting experiments. At the same time, the existing enzymatic splitting efficiency is low and the amount of use is large.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method utilizing sphingomonas to split hepatitis C ns3 enzyme inhibitor medicine intermediate
  • A kind of method utilizing sphingomonas to split hepatitis C ns3 enzyme inhibitor medicine intermediate
  • A kind of method utilizing sphingomonas to split hepatitis C ns3 enzyme inhibitor medicine intermediate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Method for splitting N-Boc-1-amino-2-vinylcyclopropane ethyl ester and 1-amino-2-vinylcyclopropane ethyl ester hydrochloride in whole cells of the strain of the present invention

[0050] (1) Strain selection: use Sphingomonas aquatilis JSS7;

[0051] (2) Seed culture: Inoculate 100 μL of the stored bacterial solution into 50 mL of seed culture medium, and culture it at 30° C. and 200 rpm for 24-48 hours to fully grow the bacteria. Observe that the culture medium is obviously turbid in color.

[0052] (3) Collect the bacteria: centrifuge the bacteria at low temperature and discard the supernatant. The cells were suspended in 10 mL of phosphate buffer for use.

[0053] (4) Transformation experiment: The substrate concentration is still 1g / L, and the transformation experiment can be completed at 30°C and 200rpm for 72 hours.

[0054] (5) Detection: Extract the reaction solution with equal volume of n-hexane, take the organic phase after centrifugation, inject 10 μL of s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for splitting a hepatitis c NS3 enzyme inhibitor drug intermediate through sphingomonasaquatilis, and particularly relates to a method for splitting hepatitis c NS3 enzyme inhibitor drug intermediate N-Boc-1-amino-2-vinylcyclopropane ethyl formate and 1-amino-2-vinylcyclopropane ethyl formate hydrochloride to prepare (1R, 2S)-N-Boc-1-amino-2-vinyl cyclopropane ethyl formate and (1S, 2R)-1-amino-2-vinyl cyclopropane ethyl formate. The method comprises the following steps that a strain is introduced into a culture medium with N-Boc-1-amino-2-vinylcyclopropane ethyl formate or 1-amino-2-vinylcyclopropane ethyl formate hydrochloride as a unique carbon source, a whole-cell catalysis substrate obtained through screening is subjected to a split reaction, and (1R, 2S)-N-Boc-1-amino-2-vinyl cyclopropane ethyl formate and (1S, 2R)-1-amino-2-vinyl cyclopropane ethyl formate are obtained.

Description

Technical field [0001] The invention relates to the use of a sphingosine to separate the hepatitis C NS3 enzyme inhibitor drug intermediate N-Boc-1-amino-2-vinylcyclopropane ethyl ester and 1-amino-2-vinyl Ethyl cyclopropane carboxylate hydrochloride to prepare (1R,2S)-N-Boc-1-amino-2-vinylcyclopropane ethyl carboxylate and (1S,2R)-1-amino-2-vinylcyclopropane Ethyl formate method. Background technique [0002] Hepatitis C virus (HCV) belongs to the Hepatitis C virus genus of the Flaviviridae family, and includes six genotypes, including I, II, and III. It has been more than 20 years since its discovery, and more than 170 million people have been infected worldwide. HCV virus includes structural proteins and non-structural proteins. Among them, the non-structural protein NS3 enzyme can directly destroy host cells and inhibit the response to interferon, which seriously affects the treatment of it. There is no safe and effective virus vaccine against HCV. Therefore, the research a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12P41/00C12N1/02C12N1/20C12R1/01
CPCC12N1/02C12N1/20C12P13/00C12P41/00
Inventor 郑国钧石莹
Owner BEIJING UNIV OF CHEM TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap