Method and reagent for detecting linezolid drug-resistant enterococcus fixed planting
A technology of linezolid and enterococcus, which is applied in the field of medical testing, can solve the problems of low positive rate, inability to effectively screen positive people, complicated process, etc., and achieves the effects of simple operation, fast testing and high sensitivity
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Embodiment 1
[0024] A method for detecting colonization of linezolid-resistant enterococci comprising the steps of:
[0025] Step S1: Heat and dissolve 58.5 g of CTCA agar in 1000 ml of distilled water, and autoclave at 121° C., and when cooled to 50-55° C., add linezolid, the concentration of which is 1.5 ug / ml, to obtain CTCA agar solution containing linezolid, wherein, the formula of the CTCA agar is tryptone 15g / L, yeast extract powder 5g / L, potassium dihydrogen phosphate 5g / L, sodium citrate 15g / L, Tween 80 1g / L, sodium carbonate 2g / L, sodium azide 0.4g / L, red tetrazolium (TTC) 0.1g / L, agar 15g / L, its pH value is 7.0 ± 0.2; CTCA agar solution is poured in the aseptic petri dish (20ml / dish) of five diameter 9cm, adopts hydrochloric acid and NaOH to adjust medium pH value to be respectively 6.0, 6.5, 7.0, 7.5 and 8.0, and five detection petri dish samples are analyzed, will It is cooled and solidified and stored in a 4-degree refrigerator for later use. Take out the plate every 7 days...
Embodiment 2
[0029] On the basis of Example 1, in this example, the concentration of the linezolid is 2ug / ml, and the others are the same as in Example 1.
Embodiment 3
[0031] On the basis of Example 1, in this example, the concentration of the linezolid is 2.5ug / ml, and the others are the same as in Example 1.
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