Method and reagent for detecting linezolid drug-resistant enterococcus fixed planting

A technology of linezolid and enterococcus, which is applied in the field of medical testing, can solve the problems of low positive rate, inability to effectively screen positive people, complicated process, etc., and achieves the effects of simple operation, fast testing and high sensitivity

Inactive Publication Date: 2016-08-17
SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the commonly used method is to monitor the growth of the bacterial flora in the stool specimen through the blood plate, select the enterococcus strain according to the morphological characteristics of the bacteria, and then conduct identification to determine whether the strain is linezolid-resistant enterococcus. Complex, low positive rate, unable to effectively screen the positive population colonized with linezolid-resistant Enterococci resistant strains

Method used

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  • Method and reagent for detecting linezolid drug-resistant enterococcus fixed planting
  • Method and reagent for detecting linezolid drug-resistant enterococcus fixed planting
  • Method and reagent for detecting linezolid drug-resistant enterococcus fixed planting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for detecting colonization of linezolid-resistant enterococci comprising the steps of:

[0025] Step S1: Heat and dissolve 58.5 g of CTCA agar in 1000 ml of distilled water, and autoclave at 121° C., and when cooled to 50-55° C., add linezolid, the concentration of which is 1.5 ug / ml, to obtain CTCA agar solution containing linezolid, wherein, the formula of the CTCA agar is tryptone 15g / L, yeast extract powder 5g / L, potassium dihydrogen phosphate 5g / L, sodium citrate 15g / L, Tween 80 1g / L, sodium carbonate 2g / L, sodium azide 0.4g / L, red tetrazolium (TTC) 0.1g / L, agar 15g / L, its pH value is 7.0 ± 0.2; CTCA agar solution is poured in the aseptic petri dish (20ml / dish) of five diameter 9cm, adopts hydrochloric acid and NaOH to adjust medium pH value to be respectively 6.0, 6.5, 7.0, 7.5 and 8.0, and five detection petri dish samples are analyzed, will It is cooled and solidified and stored in a 4-degree refrigerator for later use. Take out the plate every 7 days...

Embodiment 2

[0029] On the basis of Example 1, in this example, the concentration of the linezolid is 2ug / ml, and the others are the same as in Example 1.

Embodiment 3

[0031] On the basis of Example 1, in this example, the concentration of the linezolid is 2.5ug / ml, and the others are the same as in Example 1.

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Abstract

The invention provides a method and reagent for detecting linezolid drug-resistant enterococcus fixed planting. The method for detecting linezolid drug-resistant enterococcus fixed planting includes the following steps that CTCA agar is dissolved in distilled water, sterilization is conducted, linezolid is added when the mixture is cooled to 50-55 DEG C, the concentration of linezolid ranges from 1.5 micrograms/ml to 3 micrograms/ml, a CTCA agar solution containing linezolid is obtained and poured into a sterile plate, the pH value is adjusted to range from 7.0 to 7.5, and a detection plate is obtained; an excrement sample is taken, a flat plate of the detection plate in S1 is coated with the excrement sample, and the total colony count of enterococcus is calculated after culture is conducted for 2 days. According to the technical scheme, operation is easy, inspection is rapid, extremely high sensitivity is achieved, linezolid drug-resistant enterococcus can be directly screened out, and linezolid drug-resistant enterococcus fixed planting people can be rapidly and accurately screened out.

Description

technical field [0001] The invention belongs to the technical field of medical testing, and in particular relates to a method for detecting linezolid-resistant enterococcus colonization and a reagent thereof. Background technique [0002] Oxazolidinone drugs represented by Linezolid (LZD) are one of the main drugs for treating vancomycin-resistant Enterococci (VRE) infection and controlling the outbreak of VRE nosocomial infection. one. With the widespread application of LZD in refractory gram-positive bacteria infection and Mycobacterium tuberculosis infection in recent years, the problem of its clinical drug resistance has become increasingly prominent. Sporadic reports of drug-resistant LZD strains at home and abroad are increasing, and there are even reports of outbreaks in hospitals. CHINET data in 2011 suggested that E.fecalis, E.faecium 0.1%, E.gallinarum 1.2%, and E.avium 2.3% among LZD drug-resistant enterococci; in 2012, E.fecalis was 0.3%, E. Faecium is 0, E.fec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12R1/01
CPCC12Q1/10
Inventor 余治健邓启文程航郑金鑫陈重
Owner SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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