Detection method of rice cadmium tolerance and selection method of cadmium tolerant rice

A detection method, rice technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., can solve the problems of affecting the efficiency of detection and screening experiments, high economic costs, increasing costs, etc., to overcome the time cost and cost, simple detection method, and accurate detection results

Active Publication Date: 2020-02-04
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it takes half a year for rice to grow in one season, and the early sowing, mid-term management, fertilization and other work are also time-consuming and labor-intensive, and the economic cost is relatively high
In addition, because during the planting process, the growth of rice is easily affected by natural conditions such as changes in climate conditions, water and fertilizer, pests and diseases, so planting experiments often need to be repeated 2-3 times to obtain credible experimental results. This also further affects the efficiency of detection and screening experiments and increases the cost

Method used

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  • Detection method of rice cadmium tolerance and selection method of cadmium tolerant rice
  • Detection method of rice cadmium tolerance and selection method of cadmium tolerant rice
  • Detection method of rice cadmium tolerance and selection method of cadmium tolerant rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Through the detection method of rice cadmium tolerance in this embodiment, the cadmium tolerance exhibited by the same rice variety to solutions containing cadmium ions at different concentrations is detected. In this example, the selected rice variety is "Xiang Zao Xian 24".

[0044] In this embodiment, the TVL solution includes sorbitol at a concentration of 0.25 mol / L and calcium chloride dihydrate at a concentration of 40 mmol / L. That is, 0.25 mol of sorbitol and 40 mmol of calcium chloride dihydrate were contained in 1 L of TVL solution.

[0045] In this example, the W5 solution includes NaCl with a concentration of 150mmol / L, calcium chloride dihydrate with a concentration of 128mmol / L, KCl with a concentration of 6mmol / L and MES (pH=5.7) with a concentration of 1.5mmol / L . That is, 150 mmol of NaCl, 128 mmol of calcium chloride dihydrate, 6 mmol of KCl, and 1.5 mmol of MES (pH=5.7) were contained in 1 L of the W5 solution.

[0046] In this embodiment, the weig...

Embodiment 2

[0062] Through the detection method of rice cadmium resistance performance in this embodiment, the cadmium resistance performance of different rice varieties to the solution containing cadmium ions at the same concentration is detected, so as to screen out the rice varieties with the best cadmium resistance performance under the same conditions. . In this example, the selected rice varieties include "Xiangzaoxian 24", "Tyou 705", "Huanghuani", "Yuexiangzhan" and "Tianyou 998".

[0063] Prepare the protoplast solution of each rice variety according to the same method as in Example 1, and calculate the total number of protoplasts in the protoplast solution of each rice variety without the action of cadmium ions. The volume of the protoplast solution was 1 mL.

[0064] Take the protoplast solutions of the above five rice varieties with a volume of 1mL, and add a solution containing cadmium ions to the protoplast solutions of each rice variety, so that the concentration of cadmiu...

Embodiment 3

[0072]The method for breeding cadmium-tolerant rice in this embodiment is to use the detection method for rice cadmium-tolerant performance in the embodiment of the present invention to detect rice varieties with good cadmium-tolerant performance, and then use the screened cadmium-resistant rice for breeding to breed cadmium-resistant rice varieties. Cadmium in rice varieties. In this embodiment, cross breeding or artificial mutation breeding can be used.

[0073] Taking the "Huanghuanian" rice with the best cadmium tolerance detected in Example 2 as an example, it was cross-bred with "Huannan Early Indica" to breed a rice variety with good cadmium tolerance.

[0074] In summary, the detection method of rice cadmium tolerance in the embodiment of the present invention uses rice protoplasts to quickly and accurately detect the cadmium tolerance of rice varieties, which greatly saves time and planting costs. The method can be completed in the laboratory, so it is not affected b...

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Abstract

The invention relates to an oryza sativa cadmium tolerance detection method and a cadmium-tolerant oryza sativa selecting and breeding method and belongs to the field of selection and breeding of oryza sativa. The oryza sativa cadmium tolerance detection method includes preparing a protoplast solution containing oryza sativa protoplasts; determining the total number of the protoplasts in the protoplast solution; enabling the protoplast solution and a cadmium-ion-containing solution to interact with each other to obtain a cadmium-ion-containing protoplast solution, and determining the number of the active protoplasts in the cadmium-ion-containing protoplast solution; computing the percentage that the number of the active protoplasts accounts for the total number of the protoplasts, and using the percentage for judging cadmium tolerance of the oryza sativa. The oryza sativa cadmium tolerance detection method is capable of detecting the cadmium tolerance of the oryza sativa quickly and accurately and is not affected by factors such as moisture, climates, diseases and insect pests in the fields.

Description

technical field [0001] The invention relates to the field of rice breeding, in particular to a method for detecting cadmium-resistant performance of rice and a method for breeding cadmium-resistant rice. Background technique [0002] The content of heavy metal cadmium in the soil of some main rice producing areas in southern my country seriously exceeds the standard, and excessive cadmium content will affect the normal growth of rice and further affect the yield of rice. Since different rice varieties have significant differences in their resistance to cadmium, some cadmium-tolerant varieties of rice can grow normally even if they are planted in polluted areas. Therefore, it is very necessary to screen out rice varieties with good cadmium tolerance and promote their use in areas where cadmium exceeds the standard. [0003] The current methods for screening cadmium-tolerant rice varieties are all experimental testing through potted plants and actual planting in plots. Howev...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/06
CPCG01N33/5097
Inventor 黄永春刘仲齐张长波刘红梅
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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