Detection method of rice cadmium tolerance and selection method of cadmium tolerant rice
A detection method, rice technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., can solve the problems of affecting the efficiency of detection and screening experiments, high economic costs, increasing costs, etc., to overcome the time cost and cost, simple detection method, and accurate detection results
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Embodiment 1
[0043] Through the detection method of rice cadmium tolerance in this embodiment, the cadmium tolerance exhibited by the same rice variety to solutions containing cadmium ions at different concentrations is detected. In this example, the selected rice variety is "Xiang Zao Xian 24".
[0044] In this embodiment, the TVL solution includes sorbitol at a concentration of 0.25 mol / L and calcium chloride dihydrate at a concentration of 40 mmol / L. That is, 0.25 mol of sorbitol and 40 mmol of calcium chloride dihydrate were contained in 1 L of TVL solution.
[0045] In this example, the W5 solution includes NaCl with a concentration of 150mmol / L, calcium chloride dihydrate with a concentration of 128mmol / L, KCl with a concentration of 6mmol / L and MES (pH=5.7) with a concentration of 1.5mmol / L . That is, 150 mmol of NaCl, 128 mmol of calcium chloride dihydrate, 6 mmol of KCl, and 1.5 mmol of MES (pH=5.7) were contained in 1 L of the W5 solution.
[0046] In this embodiment, the weig...
Embodiment 2
[0062] Through the detection method of rice cadmium resistance performance in this embodiment, the cadmium resistance performance of different rice varieties to the solution containing cadmium ions at the same concentration is detected, so as to screen out the rice varieties with the best cadmium resistance performance under the same conditions. . In this example, the selected rice varieties include "Xiangzaoxian 24", "Tyou 705", "Huanghuani", "Yuexiangzhan" and "Tianyou 998".
[0063] Prepare the protoplast solution of each rice variety according to the same method as in Example 1, and calculate the total number of protoplasts in the protoplast solution of each rice variety without the action of cadmium ions. The volume of the protoplast solution was 1 mL.
[0064] Take the protoplast solutions of the above five rice varieties with a volume of 1mL, and add a solution containing cadmium ions to the protoplast solutions of each rice variety, so that the concentration of cadmiu...
Embodiment 3
[0072]The method for breeding cadmium-tolerant rice in this embodiment is to use the detection method for rice cadmium-tolerant performance in the embodiment of the present invention to detect rice varieties with good cadmium-tolerant performance, and then use the screened cadmium-resistant rice for breeding to breed cadmium-resistant rice varieties. Cadmium in rice varieties. In this embodiment, cross breeding or artificial mutation breeding can be used.
[0073] Taking the "Huanghuanian" rice with the best cadmium tolerance detected in Example 2 as an example, it was cross-bred with "Huannan Early Indica" to breed a rice variety with good cadmium tolerance.
[0074] In summary, the detection method of rice cadmium tolerance in the embodiment of the present invention uses rice protoplasts to quickly and accurately detect the cadmium tolerance of rice varieties, which greatly saves time and planting costs. The method can be completed in the laboratory, so it is not affected b...
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