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Application of TRIM66 protein in preparing non-small cell lung cancer prognosis evaluation reagent or kit

A non-small cell lung cancer, prognosis assessment technology, applied in the field of medicine, to achieve the effect of inhibiting cell proliferation

Inactive Publication Date: 2016-08-17
SHANGHAI FIRST PEOPLES HOSPITAL
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Problems solved by technology

[0007] At present, the relationship between TRIM66 gene and lung cancer has not been reported. Based on our big data analysis results, this study selected TRIM66 gene as our research target, using immunohistochemistry (IHC), RNA interference (knockdown), qRT-PCR, Western blot, CCK8 cell activity experiments, etc., verify the role of TRIM66 in the occurrence and development of lung cancer, and provide solid experimental data and potential drug targets for the early diagnosis and treatment of lung cancer

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  • Application of TRIM66 protein in preparing non-small cell lung cancer prognosis evaluation reagent or kit
  • Application of TRIM66 protein in preparing non-small cell lung cancer prognosis evaluation reagent or kit
  • Application of TRIM66 protein in preparing non-small cell lung cancer prognosis evaluation reagent or kit

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Embodiment Construction

[0027] The specific embodiments provided by the present invention will be described in detail below in conjunction with the accompanying drawings.

[0028] 1.1 Patients, tissues and cells

[0029] A total of 146 paraffin specimens of non-small cell lung cancer archived in the pathology department of our hospital from 2009 to 2010 were collected. All patients had complete clinical data and follow-up data, did not receive radiotherapy and chemotherapy before operation, and obtained informed consent. The results of pathological diagnosis were confirmed by more than two pathologists.

[0030] The cancer tissue and paracancerous tissue were taken within five minutes after the surgical specimen was excised, put into liquid nitrogen for rapid cooling, and stored in a -80°C refrigerator.

[0031] Non-small cell lung cancer A549 cell line was purchased from Shanghai Institute of Cellular Sciences, using DMEM medium containing 10% fetal bovine serum, at 37 °C, 5% CO 2 cultured in an ...

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Abstract

The invention relates to new application of TRIM66 protein, particularly application of TRIM66 protein in preparing a non-small cell lung cancer prognosis evaluation reagent or kit. The invention detects the expression rate of the TRIM66 in non-small cell lung cancer tissues is 70.5% which is higher than 51.4% of the para-carcinoma tissues. The invention detects the TRIM66 is related to the tumor size, depth of invasion, lymphatic metastasis and TNM staging of the non-small cell lung cancer, and the size of tumor, lymphatic metastasis, TNM staging and TRIM66 are independent factors for evaluating prognosis of the patients with non-small cell lung cancer. The invention also detects that the interference with the TRIM66 expression can inhibit the A549 cell proliferation.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the application of TRIM66 protein in the preparation of non-small cell lung cancer prognosis assessment reagent or kit. Background technique [0002] Lung cancer is one of the most harmful malignant tumors to human health and life in the world today. Its morbidity and mortality have shown an obvious upward trend in recent years, seriously threatening human health. In many provinces and regions of our country, the incidence and death of lung cancer have been ranking first among malignant tumors in the past ten years. Although the efficacy of surgery, chemotherapy, and radiotherapy has gradually improved, the overall 5-year survival rate is still low. With the in-depth understanding of the pathogenesis of molecular biology of lung cancer, scholars have gradually realized that the occurrence and development of lung cancer is a complex process involving multiple genes, multiple links...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/574A61K45/00A61K48/00A61K31/7088A61P35/00
CPCA61K45/00G01N33/57423G01N33/6884A61K31/7088C12Q1/6886C12Q2600/118C12Q2600/112
Inventor 韩玉栋田海英林强
Owner SHANGHAI FIRST PEOPLES HOSPITAL
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