Rapid detection method of phenolphthalein in weight reducing health foods, and detection card developing method

A technology of health food and detection method, which is applied in the direction of measuring devices, instruments, and material analysis through optical means, and can solve the problems of missed detection, high professional quality requirements of personnel, and high detection cost

Active Publication Date: 2016-08-17
北京倍肯恒业科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problems of misdetection and missing detection, only qualitative detection, high detection cost, long time-consuming, and high requirements for the professional quality of personnel in the existing detecti

Method used

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  • Rapid detection method of phenolphthalein in weight reducing health foods, and detection card developing method
  • Rapid detection method of phenolphthalein in weight reducing health foods, and detection card developing method
  • Rapid detection method of phenolphthalein in weight reducing health foods, and detection card developing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 :Quick detection method for phenolphthalein of common health food without chlorophyll and tea polyphenol

[0040] See figure 1 , figure 2 , The detection process is as follows:

[0041] 1) Pretreatment of the sample to be tested: Weigh 0.5 g of sample, put it into a 10 ml centrifuge tube, add 3 ml of extract, 0.1 g of extract, shake for 2 minutes, let stand for 15 minutes, use a microporous membrane (0.45um) Filter, take the filtrate and add an equal volume of purified water, as the sample processing solution to be tested;

[0042] 2) Qualitative testing: Add 60 microliters of the sample treatment solution to the phenolphthalein test paper card. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color scale chart for qualitative comparison;

[0043] 3) Quantitative detection: 15 minutes after the completion of the qualitative detection, the detection card is placed in ...

Embodiment 2

[0044] Example 2 :The rapid detection method of phenolphthalein in tea diet health food containing chlorophyll

[0045] See figure 1 , figure 2 , The detection process is as follows:

[0046] 1) Pre-treatment of the sample to be tested: Take about 0.5 g of the sample to be crushed, put it into a 10 ml centrifuge tube, add 3 ml of extract, 0.25 g of extract, shake for 2 minutes, stand for 15 minutes, use a microporous membrane (0.45 um) Filter, take the filtrate and add an equal volume of purified water to the sample processing solution;

[0047] 2) Qualitative testing: Add 60 microliters of the sample treatment solution to the phenolphthalein test paper card. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color scale chart for qualitative comparison;

[0048] 3) Quantitative detection: 15 minutes after the completion of the qualitative detection, the detection card is placed in the...

Embodiment 3

[0049] Example 3 :The rapid detection method of phenolphthalein in tablets containing tea polyphenols

[0050] See figure 1 , figure 2 , The detection process is as follows:

[0051] 1) Pre-treatment of the sample to be tested: Take about 0.5 g of the sample to be crushed, put it into a 10 ml centrifuge tube, add 3 ml of extract, 0.1 g of extract, and 0.1 g of extractant, shake for 2 minutes, and shake every 10 minutes 1 minute, repeat 3 times, and finally stand for 10 minutes, filter with a microporous membrane (0.45um), take the filtrate and add an equal volume of purified water to the sample processing solution;

[0052] 2) Qualitative testing: Add 60 microliters of the sample treatment solution to the phenolphthalein test paper card. If the test paper is pink, it is preliminarily proved that the sample to be tested contains phenolphthalein, which can be compared with the standard color scale chart for qualitative comparison;

[0053] 3) Quantitative detection: 15 minutes after ...

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Abstract

The invention discloses a rapid detection method of phenolphthalein in weight reducing health foods, and a detection card developing method. The rapid detection method comprises the following steps: immersing a sample to be detected in an extract liquid A, extract agent B and extract product C composition according to the type of the sample to be detected, oscillating, and standing to extract phenolphthalein; taking the obtained immersion solution, and filtering the immersion solution through a micro-porous filter membrane; taking the obtained filtered solution to be detected, adding the filtered solution to be detected to a phenolphthalein detection card in a dropwise manner, observing the change of the color of test paper, preliminarily determining that the sample contains phenolphthalein if the test paper is pink, and comparing the color with a standard color gradation spectrum to realize qualitative analysis; and using a hand-held gold marking color developing instrument to carry out quantitative detection. The detection card developing method includes the following steps: preparing a test paper substrate, preparing a color developing solution, immersing the substrate, preparing detection test paper, and preparing a detection card. The detection method is suitable for onsite rapid detection of the chemical component phenolphthalein in the weight reducing health foods, and has the advantages of low false detection and missing detection rate, realization of quantitative detection, and low requirements for testing personnel.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a rapid detection method for phenolphthalein in slimming health food and a method for developing a detection card. Background technique [0002] Phenolphthalein is a chemical product with the chemical name of 3,3-bis(4-hydroxyphenyl)-1(3H)-isobenzofuranone, and its molecular formula is C20H14O4. It is in the form of crystal powder and is almost insoluble in water. The characteristic of phenolphthalein is that it is colorless in acidic and neutral solutions, and purple in alkaline solutions, and is often used by people to detect acids and bases. Similarly, alkaline solution can also be used to detect phenolphthalein. [0003] Phenolphthalein can stimulate the colonic mucosa, enhance and prevent intestinal fluid absorption, accumulate water and electrolytes in the colon to produce a laxative effect, and promote defecation. Therefore, it is clinically used...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/17
CPCG01N21/17G01N21/78
Inventor 姚世平刘光中姚洪涛李心乐周轶
Owner 北京倍肯恒业科技发展股份有限公司
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