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Membrane separation method of sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress

A technology of heavy ion radiation and sea cucumber polysaccharide is applied in the field of membrane separation of sea cucumber polysaccharide fragments, which can solve the problems of low activity and low extraction rate of sea cucumber polysaccharide.

Inactive Publication Date: 2016-08-24
卢卫红
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of low extraction rate and low activity of sea cucumber polysaccharides, the present invention provides a method for preparing bound membrane-separated polysaccharides by pulsed electric field method, which can effectively improve the product activity of sea cucumber polysaccharides

Method used

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specific Embodiment approach 1

[0005] Specific embodiment one: In this specific embodiment, a sea cucumber polysaccharide fragment membrane separation method for simulating space heavy ion radiation oxidative stress is characterized in that it includes the following steps: the ratio of solid to liquid is 1g: 40mL-1g: 60mL Add sea cucumber powder and aqueous solution respectively, and extract with high-voltage pulsed electric field for 15 minutes to obtain the extract containing sea cucumber polysaccharides, and perform solid-liquid separation by non-isocratic centrifugation at speeds of 3000-5000r / min and 20000-25000r / min. For the extract and residue, add the residue to the aqueous solution at a ratio of 1g: 25mL-1g: 40mL, continue the pulse electric field extraction and centrifugation, and extract continuously for 3 times, remove the residue, and combine the extracts. (1) Extraction: take fresh sea cucumber vacuum freeze-dried or dried sea cucumber powder, add distilled water, the ratio of solid to liquid i...

specific Embodiment approach 2

[0006] Embodiment 2: This embodiment is different from Embodiment 1 in that the temperature of all processes in step (1) is lower than 60° C., the low-intensity electric field is 30 kilovolts per square centimeter, and the separation level of the plate-and-frame membrane is 70000-200000. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0007] Embodiment 3: This embodiment is different from Embodiment 1 in that it is characterized in that the solid content in the concentrated solution obtained in step (1) is 70-90%. Others are the same as in the first embodiment.

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Abstract

The invention relates to a preparation method of sea cucumber polysaccharides, particularly a membrane separation method of sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress. The method solves the problems of low sea cucumber polysaccharide extraction rate, indefinite active sites and complex production technique in the existing traditional process. The method comprises the following steps: drying fresh sea cucumbers and taking dry sea cucumbers, pulverizing, extracting in a high-voltage pulse electric field, separating the extracting solution by an ultracentrifuge, carrying out membrane separation on the polysaccharide fragments through a plate-frame membrane component, screening the effective fragments through in-vivo and in-vitro experiments, carrying out vacuum scraper membrane concentration, and carrying out freeze-drying by using a vacuum freeze drier, thereby obtaining the sea cucumber polysaccharide fragment powder acting on simulation space heavy ion radiation oxidation stress. The sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress are a natural extract with no side effects on the human body, have the effects of resisting oxidation, enhancing the immunity and the like, and are suitable for astronauts, air force soldiers, radar soldiers, chemoradiotherapeutic tumor patients and people with hypoimmunity. The sea cucumber polysaccharide fragments acting on simulation space heavy ion radiation oxidation stress are applicable to the fields of food, health products, cosmetics, medicine, biochemical engineering and the like.

Description

technical field [0001] The invention relates to a membrane separation method for sea cucumber polysaccharide fragments acting on simulating space heavy ion radiation oxidative stress. Background technique [0002] Sea cucumber polysaccharides are special active factors of sea cucumbers. The sea cucumber polysaccharide fragments prepared by the invention to simulate the oxidative stress of space heavy ion radiation are natural extracts, which have no side effects on the human body, have anti-oxidation, and enhance immunity, and are suitable for astronauts. , air force, radar soldiers, tumor patients undergoing radiotherapy and chemotherapy, and people with low immunity. It can be used in food, health products, cosmetics, medicine, biochemical and other fields. [0003] Although the extraction methods of sea cucumber polysaccharides are mainly ultrasonic method and reflux extraction method, a large number of free radicals will be generated during the extraction of sea cucumbe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 卢卫红徐扬
Owner 卢卫红
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