Tilapia skin protein peptide and preparation method and application thereof

The technology of fish skin protein and tilapia is applied in the directions of skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., which can solve the problems such as insufficient utilization of protein, and achieve the effects of simple operation, easy availability of raw materials and great application prospects.

Inactive Publication Date: 2016-08-31
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the traditional tilapia processing in my country, a large amount of leftovers will be produced. The utilization of these le

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Due to the high fat content of tilapia skin, it is necessary to degrease the skin first. A higher fat content will reduce the enzymatic hydrolysis effect of protease, and a small amount of fat residue will become turbid after a period of time, making it difficult to filter. Therefore, the fat treatment of tilapia skin is an indispensable step.

[0032] Take 10g of tilapia fish skin raw material, cut it into pieces, add a glass of water, stir evenly, put it in a constant temperature water bath at 30°C, add NaOH to adjust the pH value to 9.0, add 0.10% lipase in a water bath for 30 minutes, and inactivate the enzyme at a constant temperature of 82°C for 5 minutes. Rinse after cooling to obtain degreased tilapia skin.

[0033] Add water to the treated degreased tilapia fish skin according to 3 times the wet weight of the fish skin, adjust to 45°C, and adjust the pH to 7, add protease according to 0.5% of the weight of the fish skin in the raw material, enzymolysis at 45°C...

Embodiment 2

[0042] Take liquid paraffin, white vaseline, lanolin, glyceryl monostearate, and stearic acid, put them in a beaker, heat and melt in a water bath, and keep warm at 70-80°C for 1 hour. This is the oil phase. Put ethyl p-hydroxybenzoate, triethanolamine, and distilled water in another beaker and heat to melt, keep warm at 70-80°C for 1 hour, this is the water phase. Add the water phase to the oil phase in a thin stream under stirring, stir and cool to room temperature, that is, solidify into an O / W emulsion matrix. Put the protein peptide prepared in Example 1 into a mortar, grind it finely, and pass through a 60-mesh sieve. Take the matrix weight, add protein peptide powder according to the mass ratio of matrix: protein peptide is 100:3, fully stir to make tilapia fish skin protein peptide face cream.

[0043] The matrix formula is: white petrolatum 1.0g, liquid paraffin 2.4g, stearic acid 5.0g, glyceryl monostearate 2.0g, lanolin 2.0g, triethanolamine 0.2g, ethyl p-hydroxybe...

Embodiment 3

[0044] Embodiment 3: The preparation method is the same as in Example 2, except that the base formulation is: 0.5 g of white vaseline, 1.0 g of liquid paraffin, 2.0 g of stearic acid, 1.0 g of glyceryl monostearate, 1.0 g of lanolin, three Ethanolamine 0.1g, ethyl p-hydroxybenzoate 0.01g, distilled water 18g.

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PUM

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Abstract

The invention provides tilapia skin protein peptide. A preparation method of the tilapia skin protein peptide includes: S1, cutting tilapia skin into pieces, adding water, stirring, adjusting pH value to 9.0, adding lipase water bath for enzymolysis for 30 min, and cooling before rinsing to obtain defatted tilapia skin; S2, adding water of three times of wet weight of the defatted tilapia skin obtained in the S1, adjusting temperature to 30-50 DEG C, adjusting pH to 7, and adding protease for enzymolysis at constant temperature of 45 DEG C; filtering, taking supernate for concentration, and drying to obtain the tilapia skin protein peptide. Further, the invention provides a facial cream product prepared from the protein peptide. The protein peptide is high in anti-oxidation activity, activity of original protein peptide can be maintained in the facial cream product prepared from the protein peptide, added value in deep processing of tilapia is increased, and waste of resources is reduced.

Description

technical field [0001] The invention belongs to the technical field of daily necessities, and more specifically relates to a tilapia fish skin protein peptide and a preparation method and application thereof. Background technique [0002] In the traditional tilapia processing in my country, a large amount of leftovers will be produced. The utilization of these leftovers is currently limited to processing into low-value products such as salted fish meal, and the rich protein in them has not been fully utilized. [0003] The patent uses the processed tilapia leftovers-fish skin to prepare tilapia skin protein peptide face cream, which increases the added value of deep processing of tilapia, reduces waste of resources, and greatly increases the economic benefits of the enterprise. Contents of the invention [0004] The invention provides a tilapia fish skin protein peptide according to the deficiencies in the current utilization technology of tilapia leftovers. [0005] Anot...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K8/64A61Q19/00A61Q19/08
CPCA61K8/64A61Q19/00A61Q19/08C12P21/06
Inventor 邓春梅何兰珍康信煌吴湛霞吴育廉刘毅
Owner GUANGDONG OCEAN UNIVERSITY
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