Method for detecting content of triglyceride in porcine muscle with gene PPARG

A triglyceride and gene technology is applied in the field of triglyceride content detection in pig muscle, which can solve the problems of rising lean meat rate and declining pork quality.

Inactive Publication Date: 2016-08-31
GUANGXI UNIV
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However, while the lean meat percentage is r

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  • Method for detecting content of triglyceride in porcine muscle with gene PPARG
  • Method for detecting content of triglyceride in porcine muscle with gene PPARG
  • Method for detecting content of triglyceride in porcine muscle with gene PPARG

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Embodiment Construction

[0022] 1. Research Design

[0023] Taking Bamaxiang pigs as the research object, they were fed normally, and the expression levels of PPARG, SCAP and MFGE8 were detected at the age of 5 months, 8 months and 11 months, and the relationship between the expression of related genes and the content of triglyceride in pig muscle was investigated. .

[0024] Utilize the PPARG sequence (sequence accession number: GI: 47523813; see website: NCBI data http: / / www.ncbi.nlm.nih.gov / ) of the pig (sus scrofa) published in the NCBI database, SCAP (sequence accession number : GI: 927199368), MFGE8 sequence (sequence accession number: GI: 172072652), and specific quantitative primers were designed to detect the relative expression level of the gene in the pig genome. The PPARG-specific fragment of pig (sus scrofa) has a full-length sequence of 1758bp; the SCAP-specific fragment of pig (sus scrofa) has a full-length sequence of 4284bp; the MFGE8-specific fragment of pig (sus scrofa) has a full-...

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Abstract

The invention discloses a method for detecting the content of triglyceride in porcine muscle with gene PPARG. The expression level of gene PPARG in adipose tissue is utilized to detect the content of triglyceride in porcine muscle. The method has the characteristics of rapidity, accuracy, convenient operation and no influence to the normal growth of pigs, is suitable for dynamic monitoring of the content of triglyceride in porcine muscle during pig breeding so as to grasp the situation of pig growth at any time, can achieve relatively accurate understanding of the content of fat in porcine muscle, and then adjusts the daily ration of pigs.

Description

technical field [0001] The invention belongs to the field of detection of triglyceride content in pig muscle, in particular to a method for detecting triglyceride content in pig muscle with gene PPARG. Background technique [0002] Pigs are the main meat animals in my country, and pork production has accounted for more than 60% of the total meat production. The breeding work over the years has greatly improved the level of pig lean meat and significantly improved fat traits. However, while the lean meat rate is rising, the quality of pork is declining sharply. [0003] The fat in pig muscle is usually called intramuscular fat (IMF). The fat content in pig muscle is an important economic trait and an extremely important indicator affecting meat quality. have an important impact. Adipose tissue can store a large amount of triglycerides, and the main component of intramuscular fat in pigs is triglycerides. By measuring triglycerides, we can understand the accumulation of fat...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 周磊邓洛娜褚毅李一星
Owner GUANGXI UNIV
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