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Application of miR-124 in mammary cancer bone metastasis diseases

A technology for breast cancer and bone metastasis, applied in the field of biomedicine to achieve the effect of preventing breast cancer cell metastasis

Inactive Publication Date: 2016-08-31
蔡维泺 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the role of miR-124 in breast cancer bone metastasis has not been reported

Method used

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  • Application of miR-124 in mammary cancer bone metastasis diseases
  • Application of miR-124 in mammary cancer bone metastasis diseases
  • Application of miR-124 in mammary cancer bone metastasis diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 The role of miR-124 in bone metastasis of breast cancer

[0066] 1. Materials

[0067] (1) cells

[0068] Human breast cancer cell lines MDA-MB-231, Hs578t, BT549, BT474, MDA-MB-436, MDA-MB-468, MCF7, and T47D cells were purchased from Wuhan Boster Biotechnology Co., Ltd. Mouse macrophages RAW264.7 and mouse osteoblast precursor cells 3T3E1 were donated by postdoctoral Li Zhenhui from the School of Bioengineering, East China Normal University. The culture condition of MDA-MB-231, Hs578t, BT549, BT474, MDA-MB-436, MDA-MB-468, RAW264.7 and 3T3E1 cells was DMEM containing 10% fetal bovine serum. The culture condition of MCF7 and T47D cells was 1640 medium containing 10% fetal bovine serum.

[0069] (2) Animals

[0070] Female BALB / c mice used in the experiment were purchased from the Experimental Animal Center of Second Military Medical University, clean grade. Used to isolate normal mouse mammary gland tissue and construct mouse bone metastasis model.

[007...

Embodiment 2

[0148] Example 2 Molecular mechanism of miR-124 inhibiting bone metastasis of breast cancer

[0149] 1. Materials

[0150] (1) cell line

[0151] The human embryonic kidney (HEK) 293 cell line was provided by the School of Bioengineering, East China Normal University.

[0152] (2) Primer design

[0153] All primers were designed for human genes, and the primers were synthesized by Shanghai Sangon Bioengineering Co., Ltd.

[0154]

[0155] 2. Experimental method

[0156] (1) Real-time PCR

[0157] Refer to the relevant experiments in the Examples section.

[0158] (2) Western blot

[0159] It is mainly used to detect the expression of IL11 protein in breast cancer cells.

[0160] 1. Electrophoresis: take an appropriate amount of RIPA lysate to lyse the cells, measure the protein concentration, configure 10% PAGE gel, and take 50 μg of total protein from each component for electrophoresis. Stop electrophoresis after judging that the target protein has been sufficientl...

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Abstract

The invention relates to application of miR-124 in mammary cancer bone metastasis diseases. The application comprises application of miR-124 in preparing tools for prognosing mammary cancer bone metastasis risk and diagnosing whether mammary cancer metastasis occurs, and application of miR-124 in preparing drugs for preventing and / or treating mammary cancer bone metastasis. In the invention, the expression of the miR-124 in mammary cancer cells with different bone metastasis capabilities and mouse mammary cancer bone metastasis tissues is detected to determine the relationship between the miR-124 and mammary cancer bone metastasis preliminarily; the expression of the miR-124 in tumor cells is exogenously enhanced or inhibited, and the actions of the miR-124 on osteoblasts and osteoclasts are observed, thereby further determining that the miR-124 can inhibit the mammary cancer bone metastasis by regulating the interactions between the mammary cancer cells and the bone microenvironment. The invention verifies the actions of the miR-124 in mammary cancer bone metastasis, and provides a new target for treating mammary cancer bone metastasis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of miR-124 in diagnosing bone metastasis of breast cancer and preparing drugs for inhibiting bone metastasis of breast cancer. Background technique [0002] Breast cancer is the most common malignant tumor among women in my country. Its incidence rate is the first among female tumors, and its mortality rate is the second. Although advances in diagnosis and treatment have made early-stage breast cancer easier to diagnose and have standard treatment options, survival rates have also improved. However, patients with advanced breast cancer often develop local progression or distant metastasis, and there is still no recognized standard of treatment, and the survival time has not been significantly improved. The most common site of distant metastasis of breast cancer is the bone. In patients with advanced breast cancer, the incidence of bone metastasis is as high ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C40B40/06A61K45/00A61P35/04
CPCC12Q1/6886A61K45/00C12Q2600/158C12Q2600/178C40B40/06
Inventor 蔡维泺
Owner 蔡维泺
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