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A method to improve the extraction rate of dietary fiber from highland barley based on response surface methodology
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A dietary fiber and response surface methodology technology, applied in food extraction, food science and other directions, can solve the problems of poor physical and chemical properties and physiological functions of dietary fiber, cumbersome extraction steps, low extraction rate, etc., to improve reliability and accuracy. efficiency, improve production efficiency and product quality, and the effect of simple steps
Active Publication Date: 2020-01-07
SHANGHAI JIAOTONG UNIV
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However, the extraction rate of these methods is low, the cost is high, and the extraction steps are cumbersome. At the same time, they have an impact on the physical and chemical properties and physiological functions of dietary fiber. They are not suitable for industrial extraction of dietary fiber from highland barley.
At this stage, there are few studies on the optimization of the extraction method of dietary fiber from highland barley, and even less research on the optimization of the extraction method of dietary fiber from highland barley by hydrogen peroxide. At present, there are still many areas to be improved in the extraction of dietary fiber from highland barley
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Embodiment 1
[0058] In this embodiment, the hydrogen peroxide extraction method and preparation of highland barley dietary fiber include the following steps:
[0059] Step 1. Weigh 10g of dry weight powder of Nanlonggana highland barley (sieved with a 5mm aperture sieve after grinding the highland barley), place it in a beaker, add 80ml of distilled water, stir evenly with a glass rod, and place it in a water bath to keep it warm to 80 ℃;
[0060] Step 2. Add 50 microliters of high-temperature resistant α-amylase, and enzymatically hydrolyze in a water bath at 80°C for 1 hour;
[0061] Step 3. Adjust the temperature of the water bath to 60°C, weigh 15㎎ of papain and add the enzymolysis solution, and enzymatically hydrolyze in a water bath at 60°C for 1 hour;
[0062] Step 4. Inactivate in a water bath at 100°C for 30 minutes, and then adjust the temperature back to 60°C;
[0063] Step 5. Add 400 microliters of glucoamylase and enzymatically hydrolyze for 1 hour at 60°C in a water bath;
[0064] Step...
Embodiment 2
[0072] This example relates to a water extraction method and preparation of highland barley dietary fiber. The method is the same as Example 1, except that:
[0073] The heat preservation time of the water bath in step 6 is 12h;
[0074] Implementation effect, under the experimental conditions, the extraction rate of highland barley dietary fiber was 38.12%.
Embodiment 3
[0076] This embodiment relates to a hydrogen peroxide extraction method and preparation of highland barley dietary fiber. The method is the same as that in Example 1, except that:
[0077] In step 6, the pH is adjusted to 9.15, the holding time of the water bath is 12h, and the hydrogen peroxide concentration is 1.5%;
[0078] Implementation effect: Under the experimental conditions, the extraction rate of highland barley dietary fiber was 55.46%.
[0079] Optimization part: Example 1
[0080] The hydrogen peroxide extraction method and preparation of highland barley dietary fiber optimized by response surface method in this embodiment, the method includes the following steps:
[0081] Step 1, under the conditions of pH value: 6.5, 9.15; extraction time: 10h, 12h; hydrogen peroxide concentration: 0.5%, 1%, the extraction experiment of highland barley dietary fiber. The extraction method of highland barley dietary fiber is: weigh 10g dry weight of Nanlongna highland barley powder (the ...
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Abstract
The invention discloses a method for improving the highland barley dietary fiber extraction rate based on a response surface method. The method comprises the following steps of: designing extraction relevant factor levels, performing hydrogen peroxide extraction on highland barley dietary fibers, calculating the extraction rate, and determining the optimal factor level according to the obtained extraction rate; analyzing the significance of the optimal factor level on the extraction rate by virtue of a Plackeet-Burman experiment; performing a steepest ascent experiment according to the significance analysis result, and determining an experiment central point of the response surface method; performing an experiment according to the experiment central point, performing hydrogen peroxide extraction on highland barley dietary fibers, and obtaining extraction rates of dietary fibers under various factor levels; constructing a three-dimensional quantitative structure-property model of the response surface method according to the extraction rates obtained in the step 4; and predicating the optimal factor level according to the model, and performing hydrogen peroxide extraction on highland barley dietary fibers by use of the factor level, thereby obviously improving the extraction rate. The method is simple and easy to realize, can be used for improving the productivity and the product quality and lowering the cost, and is green, free from chemical reagents and less in damage on highland barley dietary fiber structures, thereby being an environment-friendly technology.
Description
Technical field [0001] The invention belongs to the field of food, and specifically relates to a hydrogen peroxide extraction method of highland barley dietary fiber optimized by a response surface method, in particular to a method for improving the extraction rate of highland barley dietary fiber based on a response surface method. Background technique [0002] Dietary fiber is a kind of polysaccharide. It can neither be digested and absorbed by the gastrointestinal tract, nor can it produce energy. Therefore, it was once regarded as a "non-nutritive substance" and has not received enough attention for a long time. However, with the in-depth development of nutrition and related sciences, people have gradually discovered that dietary fiber has a very important physiological role. Highland barley has rich nutritional value and outstanding medical and health care effects. In the high-cold and hypoxic Qinghai-Tibet Plateau, why there is no shortage of centenarians? This is insepar...
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