The invention discloses a method for extracting water-soluble dietary fibers from lentinus edodes stems. The method includes the following steps that 1, the lentinus edodes stem raw materials are dried, smashed and then sieved, and uniform lentinus edodes stem powder is obtained; 2, a buffer solution is added into the lentinus edodes stem powder, a cellulase solution is added, the materials are stirred to be uniform for enzymatic hydrolysis, enzyme deactivation is carried out after enzymolysis is completed, and reaction liquid is obtained; 3, the reaction liquid is centrifuged for solid-liquid separation, supernatant liquid is taken for concentration, and concentrated liquid is obtained; 4, absolute ethyl alcohol is added into the concentrated liquid, after the materials are fully mixed, the mixture stands overnight, suction filtration is carried out, sediment products are obtained and dried to constant weight, and the water-soluble dietary fibers are obtained. The factors, affecting the extraction rate of the water-soluble dietary fibers, of the enzymolysis temperature, use amount, mesh number and enzymolysis time of cellulase are optimized, so that the extraction rate of the water-soluble dietary fibers is increased, and the extracted water-soluble dietary fibers have high water-holding power.