Method for extracting water-soluble dietary fibers from lentinus edodes stems

Abstract

The invention discloses a method for extracting water-soluble dietary fibers from lentinus edodes stems. The method includes the following steps that 1, the lentinus edodes stem raw materials are dried, smashed and then sieved, and uniform lentinus edodes stem powder is obtained; 2, a buffer solution is added into the lentinus edodes stem powder, a cellulase solution is added, the materials are stirred to be uniform for enzymatic hydrolysis, enzyme deactivation is carried out after enzymolysis is completed, and reaction liquid is obtained; 3, the reaction liquid is centrifuged for solid-liquid separation, supernatant liquid is taken for concentration, and concentrated liquid is obtained; 4, absolute ethyl alcohol is added into the concentrated liquid, after the materials are fully mixed, the mixture stands overnight, suction filtration is carried out, sediment products are obtained and dried to constant weight, and the water-soluble dietary fibers are obtained. The factors, affecting the extraction rate of the water-soluble dietary fibers, of the enzymolysis temperature, use amount, mesh number and enzymolysis time of cellulase are optimized, so that the extraction rate of the water-soluble dietary fibers is increased, and the extracted water-soluble dietary fibers have high water-holding power.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for extracting water-soluble dietary fiber from mushroom stalks. Background technique [0002] In November 1999, in the 84th session of the American Association of Cereal Chemists (AACC, American Association of Cereal Chemists), after many discussions, dietary fiber was defined as: Dietary fiber refers to the ability to resist digestion and absorption in the small intestine of the human body, and partially or in the large intestine of the human body. The total of whole fermented edible vegetable components, carbohydrates and their analogues, including polysaccharides, oligosaccharides, lignin and related plant matter. It can be roughly divided into two categories: water-soluble dietary fiber (Soluble dietary fiber, SDF) and water-insoluble dietary fiber (Insoluble dietary fiber, IDF). In terms of physiological activity, SDF can delay digestion, help diabetic pat...

AI Technical Summary

Problems solved by technology

However, although the chemical extraction method is simple and fast, the quality of the obtained product is generally poor, showing poor color and more impurities. Environmentally polluting and therefore not advisable approach

Huang Maokun used orthogonal experiments to initially explore the use of cellulase to modify mushroom stalks, but he did not take into account the impact of the particle size of the material during the extraction of soluble dietary fiber on the test, and the extraction rate of soluble dietary fiber was relatively low.

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