A kind of Artemisia annua homogeneous polysaccharide and its preparation method and application
A technology of Artemisia annua and polysaccharide, applied in the field of Artemisia annua homogeneous polysaccharide and preparation thereof, can solve the problem of no extraction of Artemisia univaries polysaccharide, little active effect of Artemisia annua polysaccharide, no Artemisia Artemisiae homogeneous polysaccharide research report on rheumatoid arthritis, etc. The problem is that the preparation method is simple and the effect of reducing the swelling degree of the foot is achieved.
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Embodiment 1
[0037] Example 1: Preparation and identification of the homogeneous polysaccharide of Artemisia annua
[0038] 1.1 Preparation of homogeneous polysaccharide from Artemisia annua
[0039] a) Take 2 kg of dried Artemisia annua shredded, add 20 L of ethanol with a mass fraction of 75% to reflux degrease (to remove low-polarity components), reflux degrease after 10 hours, filter to obtain an ethanol-insoluble residue, and dry Residue, then add boiling water, reflux extraction twice, each time 4h, the ratio of solid to liquid for the first extraction is 1:12 (ie: 1Kg residue, use 12L of water), the ratio of solid to liquid for the second extraction is 1:10 (i.e.: 1Kg residue, use 10L water); filter, combine two extracts, concentrate, add ethanol with a mass fraction of 95%, stir while adding, until the mass fraction of final ethanol is 75%, let it stand for alcohol precipitation for 24 hour, filter, collect the precipitate and wave off the residual ethanol; centrifuge the precipit...
Embodiment 2
[0064] Example 2: In vivo pharmacodynamic experiments in mice with arthritis
[0065] 1) Establishment of collagen-induced arthritis mouse model:
[0066] Animals: 6-8 weeks old, 12 female DBA / 1 mice, bred in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.
[0067] Reagents: bovine type II collagen (CII), complete Freund's adjuvant (CFA), and incomplete Freund's adjuvant (IFA) were purchased from Chondrex, USA.
[0068] Methods: For the first immunization, 100 μL of CFA emulsion containing 100 μg CⅡ was injected intradermally at the base of the tail of DBA / 1 mice, which was recorded as the first day, and a booster immunization was performed on the 22nd day, and CFA containing 100 μg CⅡ was injected intradermally. The emulsion of IFA was 100 μL, and the clinical manifestations of the mice were observed.
[0069] Scoring: scoring every two days by two independent observers; scoring criteria: 0, no erythema and edema; 1, erythema and mild ...
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