A fermentation accelerator for preparing feed from air-dried bagasse

A fermentation accelerator, bagasse technology, applied to bacteria, animal feed, animal feed and other directions used in food preparation, can solve the problem of restricting the development of lignocellulosic biomass conversion feed, low degradation rate of lignocellulosic enzymes, and promoting fermentation. It can reduce the loss, have good adaptability, and have strong acid-producing ability.

Inactive Publication Date: 2018-07-20
KUNMING UNIV OF SCI & TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low degradation rate of lignocellulosic enzymes, the development of lignocellulosic biomass conversion feed has been gradually limited. In addition, there are many limitations in simply improving the protein evaluation index of feed products. Therefore, how to start from It is more important to improve the flavor of bagasse feed and increase animal feed intake to prepare more promising feed products. At the same time, this idea has gradually been widely accepted by people.
[0004] At present, the commercially available starters are mainly aimed at the production of green straw and straw feed, and the relevant researches are relatively mature. However, there are still few researches on fermentation accelerators for the preparation of air-dried cellulosic biomass. Therefore, the use of air-dried cellulose Biomass is used to prepare feed, so the demand for its fermentation accelerator is extremely urgent
[0005] Yunnan Province is one of the main sugar producing areas in the country. The province has more than 80 sugar-making enterprises, covering 14 prefectures and states in the province, involving more than 40 counties. A large amount of bagasse is produced every year. The current common practice is 90% bagasse It is used as fuel for power generation and steam supply in sugar factory boilers, and the remaining 10% of bagasse is mainly used for papermaking and animal feed production, which not only pollutes the environment, but also greatly wastes resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A fermentation accelerator for preparing feed from air-dried bagasse
  • A fermentation accelerator for preparing feed from air-dried bagasse
  • A fermentation accelerator for preparing feed from air-dried bagasse

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] S3, the preparation of fermentation accelerator: after each bacteria reaches the corresponding effective viable count, mix by volume ratio of 1:1:1:3-5 to make fermentation accelerator;

[0030] S4. Preparation of fermentation material: use MRS-S medium to adjust the moisture content of air-dried bagasse crushed material to 65-75%, add 0.5% urea, 1% ammonium sulfate, 8% sucrose by dry weight, stir evenly, and make fermentation material ;

[0031] S5. Anaerobic fermentation: The bacterial liquid of the fermentation promoter in S3 is evenly sprayed on the fermentation material in S4 according to the concentration ratio of 10%, stirred evenly, strictly compacted, sealed with three layers of plastic film, solid anaerobic at 33-37 ° C Fermentation promoters for the desired feeds can be prepared after 20 to 25 days of fermentation.

[0032] In this embodiment, the fermentation material can also be prepared by using air-dried bagasse and molasses alcohol waste mash. Specifica...

Embodiment 1

[0033]Embodiment 1, fermentation accelerator is prepared as follows:

[0034] 1) Inoculate Lactobacillus plantarum stored at -80°C into 100mL MRS-S medium at 37°C for anaerobic culture for 24 hours for activation, and then expand the culture with 10% of the inoculum;

[0035] 2) Inoculate Pediococcus lactis stored at -80°C into 100mL MRS-S medium at 37°C for anaerobic culture for 24 hours for activation, and then expand the culture with 10% of the inoculum;

[0036] 3) Inoculate Streptococcus faecalis stored at -80°C into 100Ml MRS-S medium at 37°C for anaerobic culture for 24 hours for activation, and then expand the culture with 10% of the inoculum;

[0037] 4) Inoculate Bacillus subtilis stored on a -4°C slant into 100mL LB medium, 37°C shake flask culture for 24 hours for activation, and then expand the culture with 10% of the inoculum;

[0038] 5) Preparation of fermentation accelerator: Lactobacillus plantarum, Pediococcus lactis, Streptococcus faecalis, mixed and prepa...

Embodiment 2

[0043] Embodiment 2 The influence of different lactic acid bacteria fermentation accelerators on the fermentation effect of air-dried bagasse

[0044] (1) prepare lactic acid bacteria fermentation accelerators A, B, C, D, E, F, G;

[0045] (2) the bagasse is air-dried and pulverized to 40 meshes;

[0046] (3) Load the bagasse pulverized material into a plastic bucket with a diameter of 30cm and a height of 40cm, adjust the moisture content of the air-dried bagasse pulverized material to 65-75% by using MRS-S medium, add 8% sucrose and 0.5% urea by dry weight , 1% ammonium sulfate, stir evenly, prepare 8 portions, spray fermentation accelerators A, B, C, D, E, F, G respectively, stir evenly, seal and compact with plastic film multiple times to ensure anaerobic environment, seal After 30 days of fermentation, open the sealing film and take samples; the uninoculated group is marked as H;

[0047] (4) Take 200g of each sample in this example A, B, C, D, E, F, G, and H for sensor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a fermentation accelerator using air-dried bagasse to prepare feed. The preparation method is as follows: S1, strain expansion: expand and cultivate Lactobacillus plantarum, Pediococcus lactis, Streptococcus faecalis and Bacillus subtilis respectively; S2, Preparation of lactic acid bacteria fermentation accelerator: after the number of effective viable bacteria in S1 reaches the corresponding number, mix according to volume ratio 1:1:1:3‑5 to make lactic acid bacteria fermentation accelerator bacterial liquid; S3, preparation of fermentation material: use MRS ‑S medium adjusts the moisture content of air-dried bagasse pulverized material to 65‑75%, add 0.5% urea, 1% ammonium sulfate, 8% sucrose in dry weight, stir evenly, and make fermentation material; S4, anaerobic fermentation: mix The lactic acid bacteria fermentation accelerator bacterial liquid in S2 is evenly sprayed on the fermentation material in S3 at a concentration ratio of 10%, and the fermentation accelerator for the required feed can be obtained by solid-state anaerobic fermentation at 33-37°C for 20-25 days.

Description

technical field [0001] The invention relates to a ruminant feed, in particular to a fermentation accelerator for preparing feed by using air-dried bagasse. Background technique [0002] As an important part of agriculture, animal husbandry is of great significance to strengthening the scientific and technological innovation of animal husbandry and promoting the transformation and application of scientific and technological achievements. my country is the world's largest producer of sugarcane, with an annual output of 70 million tons of sugarcane, ranking third in the world after Brazil and India. Bagasse is a by-product of the sugar industry. It is the solid waste residue left after the sugarcane is squeezed to extract juice. Generally speaking, 1 ton of sugarcane can produce 25-300kg of bagasse. Bagasse mainly includes components such as cellulose, hemicellulose and lignin, and also contains a small amount of protein, ash and wax. Therefore, bagasse has great potential fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/10A23K10/37A23K10/12
CPCA23V2400/225A23V2400/169Y02P60/87
Inventor 余旭亚张和平尚敏敏车饶琼丁柯赵永腾
Owner KUNMING UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products