Centrifugal type multi-channel microfluidic chip

Abstract

The invention discloses a centrifugal type multi-channel microfluidic chip; a first layer surface of the chip is provided with a first fluid suite including first reaction tanks, flow paths and solution injection ports, and each flow path is provided with a first solution temporary storage zone for conveying a liquid to the first reaction tank; each flow path includes a first flow path and branches, and each first flow path extends from a valley to the first reaction tank to form a first communication point; the branches and each first reaction tank form a second communication point, and the branches extend from the second communication point to an outlet end along the direction toward a rotation center. The fluid devices of the first layer surface can communicate with those of a second surface layer through channels which communicate with each other in an upper-and-lower manner, and the first fluid suite and a second fluid suite can communicate with each other. The microfluidic chip has an ingenious structure; substances and auxiliary ingredients which can undergo reactions with different substances or ingredients in a to-be-detected sample can be pre-loaded in different reaction devices; reaction results are obtained through one time or multiple times of solution addition.

Description

technical field [0001] The present invention relates to a sample analysis fluid chip, a sample analysis method, and a method for manufacturing a sample analysis fluid chip used for detection or analysis of biological reactions. In particular, it relates to a disposable biochip that can be used for protein or DNA analysis and a method for producing the same. Background technique [0002] In the past, for example, in the field of biochemical reactions such as DNA reaction and protein reaction, as a reaction device for processing a small amount of sample solution, there are known micro-total analysis systems (μ-TAS; Total Analysis System) or lab-on-a-chip (Lab-on-a-chip). on-Chip) technology. This technology provides multiple reaction chambers (hereinafter also referred to as "reaction cells") or flow paths to one chip or reaction cartridge, and enables analysis of multiple detection formations or multiple reactions. These technologies have been considered to have various adv...

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Problems solved by technology

The design of the main channel, that is, the design of the main channel with the same width between the mountain and the valley still fails to solve the problem of the uniformity of liquid dosing in each sampling hole; in addition, the design of the valley is too wide than the mountain, resulting in There is a problem of air bubbles in the main flow

In addition, by adding the waste liquid chamber to carry excess solution, although the consistency of the liquid volume distributed in each sample hole is achieved to a certain extent, it cannot guarantee that the liquid is always full of the sample hole during the reaction process, and it needs to be filled in the microfluidic pipeline. Local surface modification treatment is carried out inside, that is, the hydrophilic treatment of the inner surface of the branch flow channel connected to the sample hole, and the hydrophobic treatment of the inner surface of the branch flow channel connected with the waste liquid chamber that collects the remaining solution, which increases the complexity of chip manufacturing. Sex, processing cost and processing difficulty

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