EDTA-citric acid antigen repair fluid

A technology of citric acid monohydrate and repair solution, applied in measuring devices, instruments, scientific instruments and other directions, can solve the problems of volatile, antigen destruction, drying of tissue slices, etc., and achieve the effect of non-volatile and good repair effect.

Active Publication Date: 2016-10-05
ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The solvent of the above-mentioned antigen retrieval solution is water. If it is applied to an automatic immunohistochemical staining instrument, on the one hand, the antigen retrieval solution needs to be used in a large amount to achieve a good repair effect, and the tissue section canno...

Method used

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Examples

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Effect test

Embodiment 1

[0029] An EDTA-citric acid antigen retrieval solution, each 1000mL EDTA-citric acid antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, citric acid monohydrate 0.378252g, sodium citrate dihydrate 2.411784g, Tween -20 500 microliters, 150mL triethanolamine and the remainder of distilled water.

[0030] The preparation method is as follows: Weigh Tris-Base, EDTA, citric acid monohydrate, and sodium citrate dihydrate according to the proportions, and dissolve Tris-Base, EDTA, citric acid monohydrate, and sodium citrate dihydrate in mono-distilled water, and then add the formula After the amount of triethanolamine is completely dissolved, add Tween-20, and finally distilled water to the rated capacity.

Embodiment 2

[0032] An EDTA-citric acid antigen retrieval solution, each 1000mL EDTA-citric acid antigen retrieval solution includes: Tris-Base 1.2114g, EDTA 0.37224g, citric acid monohydrate 0.378252g, sodium citrate dihydrate 2.411784g, Tween -20 500 microliters, 325mL of ethylene glycol and the remainder of distilled water.

[0033] The preparation method is as follows: Weigh Tris-Base, EDTA, citric acid monohydrate, and sodium citrate dihydrate according to the proportions, and dissolve Tris-Base, EDTA, citric acid monohydrate, and sodium citrate dihydrate in mono-distilled water, and then add the formula After the amount of ethylene glycol is completely dissolved, add Tween-20, and finally dilute to the rated capacity with distilled water.

Embodiment 3

[0035] An EDTA-citric acid antigen retrieval solution, each 1000mL EDTA-citric acid antigen retrieval solution includes: Tris-Base 1.20g, EDTA 0.35g, citric acid monohydrate 0.35g, sodium citrate dihydrate 2.40g, Tween -20 400 microliters, 50mL triethanolamine and the remainder of distilled water.

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Abstract

The invention relates to the technical field of immunohistochemistry and discloses an EDTA-citric acid antigen repair fluid. Each 1000mL EDTA-citric acid antigen repair fluid comprises 1.20-1.25g of Tris-Base, 0.35-0.40g of EDTA, 0.35-0.40g of citric acid monohydrate, 2.40-2.42g of trisodium citrate dehydrate, 400-600mL of tween-20, 50-500mL of triethanolamine, ethylene glycol or trihydric alcohol and the balance of single steam water. The EDTA-citric acid antigen repair fluid provided by the invention has excellent wetting and repairing effects; a little dosage of EDTA-citric acid antigen repair fluid can be used for repairing tissue slice; the repaired tissue slice is easy to dye; the dyeing effect is excellent; the repair fluid is difficult to volatilize and can be used together with a full-automatic immunohistochemical instrument.

Description

Technical field [0001] The invention relates to the technical field of immunohistochemistry, in particular to an EDTA-citrate antigen retrieval solution. Background technique [0002] Immunohistochemistry technology is a detection technology that has developed rapidly in pathological diagnosis in recent years. As an important pathological auxiliary diagnosis method, it has received increasing attention in clinical applications. With the application and promotion of automatic immunohistochemical staining apparatus, the requirements for staining quality are also increasing. Among them, antigen retrieval plays an extremely important role in the entire immunohistochemical staining process, and the quality of the repair conditions directly determines the quality of the final staining result. Because of its own structural characteristics, the automatic staining instrument has a small volume for holding the repair solution, and it cannot completely immerse the tissue section in the rep...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N33/531
CPCG01N1/30G01N33/531
Inventor 李思勇余向东
Owner ZHEJIANG CENTURY CONDOR MEDICAL SCI & TECHNOLOTY CORP
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