Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering

A self-healing and hydrogel technology, applied in the application field of biological tissue engineering, can solve the problems of reduced service life of materials, achieve increased stability, simple and controllable synthesis method, and improve application prospects

Active Publication Date: 2016-10-12
深圳智凝高分子材料科技有限公司
View PDF7 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the self-healing function is one of the most remarkable features of organisms, but in synthetic materials, due to the degradation of mic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering
  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering
  • Injectable self-repairing hydrogel based on polysaccharides, preparation method and application of hydrogel to biological tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1:

[0022] 1) 2.05g chitosan is dissolved in 100mL distilled water under stirring at room temperature. Acrylic acid 1.5mL was slowly added dropwise, the temperature was raised to 50°C, and the reaction was magnetically stirred for 3 days to obtain a light yellow clear viscous liquid. The obtained polymer solution was cooled to room temperature, and 1M sodium hydroxide was added to adjust the pH of the system to 12. Dialysis with a dialysis bag with a molecular weight of 14000 for 3 days, and constantly changing the water. The polymer solution after dialysis is used to remove most of the water with a rotary evaporator to obtain a clear liquid with a certain viscosity. Freeze drying at -50°C for 3 days to obtain a fluffy and porous N-carboxyethyl chitosan (N-carboxyethyl chitosan) solid powder A with a yield of about 75%.

[0023] 2) Take 1.00 g of sodium hyaluronate, stir and dissolve in 100 mL of distilled water at room temperature, add sodium periodate (NaIO 4 )...

Example Embodiment

[0026] Example 2:

[0027] 1) 2.05g of ethylene glycol chitosan is stirred and dissolved in 100mL of distilled water at room temperature. Acrylic acid 1.5mL was slowly added dropwise, the temperature was raised to 50°C, and the reaction was magnetically stirred for 3 days to obtain a light yellow clear viscous liquid. The obtained polymer solution was cooled to room temperature, and 1M sodium hydroxide was added to adjust the pH of the system to 12. The dialysis bag with a molecular weight of 8000 was used for dialysis for 3 days, and the water was changed constantly. The polymer solution after dialysis is used to remove most of the water with a rotary evaporator to obtain a clear liquid with a certain viscosity. Freeze drying at -50°C for 3 days to obtain a fluffy and porous N-carboxymethyl glycol chitosan solid powder A with a yield of about 75%.

[0028] 2) Take 1.00 g of dextran sulfate, stir and dissolve it in 100 mL of distilled water at room temperature, add sodium period...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses injectable self-repairing hydrogel based on polysaccharides, a preparation method and application of the hydrogel to biological tissue engineering, and belongs to the technical field of high polymer materials. The method includes the steps of modifying natural high polymer chitosan so that the defect that the chitosan can only be dissolved in acid solutions can be overcome and the dissolubility of the chitosan in water can be improved; conducting mixing reaction on the chitosan and a modified sodium hyaluronate derivative solution, delaying the reaction time or raising the temperature so that sol can be converted into gel and hydrogel can be endowed with the injection characteristic and the self-repairing characteristic. It is shown through cell co-culture experiments that the intelligent hydrogel has the advantage of high biocompatibility, degradability and the like. The injectable self-repairing hydrogel as a novel medical high polymer carrier material has potential biological and medical application value when used for the co-culture together with cells, protein, DNA, antigens and other active substances, and even the delivery, release and other aspects of medicine and other in-vitro substances.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a polysaccharide-based injectable self-repairing hydrogel, a preparation method and its application in biological tissue engineering. Background technique [0002] In recent years, the research work on smart materials has been unprecedentedly active, among which the development of new soft matter materials with smart phase change behavior is the fastest. Such materials can sense changes in surrounding environmental conditions, such as temperature, acid-base, pressure, sound waves, electric fields, magnetic fields, and light waves, and produce corresponding phase changes at the same time. Hydrogels are one of them. It is a three-dimensional network structure composed of two types of groups, hydrophilic and hydrophobic. The hydrophilic part absorbs a large amount of water, giving the hydrogel fluidity similar to liquid; at the same time, the hydrophobic part ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08J3/075C08L71/02C08L5/08C08L5/02C08B37/08C08B37/02C08G65/332A61L27/26A61L27/52
CPCA61L27/26A61L27/52C08B37/0006C08B37/003C08B37/0072C08G65/3322C08G2650/04C08J3/075C08J2371/02C08J2405/02C08J2405/08C08L71/02C08L2201/06C08L2203/02C08L2205/02C08L2205/025C08L2205/03C08L2312/06C08L5/08C08L5/04C08L5/02
Inventor 林权赵月杨旭东王威姜英男张川杨柏
Owner 深圳智凝高分子材料科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap