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This invention belongs to the field of polymer functional materials technology, specifically relating to a biodegradable light-converting film with intrinsic fluorescence and its applications. The biodegradable light-converting film is prepared from a biodegradable copolyester with aggregation-induced emission properties. The biodegradable copolyester is polymerized from a polymerizable monomer, a first monomer providing rigid conjugated units, and a second monomer providing flexible spacer units. In the main chain structure of the biodegradable copolyester, the longest linear spacer segment between any two adjacent rigid conjugated units derived from the first monomer has ≥10 carbon atoms. Through precise molecular structure design, the aggregation-induced emission properties are chemically bonded to the biodegradable polyester main chain, fundamentally solving the problems of easy migration, aggregation quenching, and high cost of the light-converting agent in traditional additive-type light-converting films. This light-converting film exhibits efficient and stable intrinsic fluorescence in the solid state, with tunable fluorescence color, and possesses good mechanical properties and biodegradability.