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A kind of method of selection chicken stress resistance performance

A performance and rooster technology, applied in the field of breeding industry, to achieve the effects of high survival rate, high egg white lysozyme content, and high hatching rate

Active Publication Date: 2019-10-11
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of lysozyme gene to carry out chicken stress resistance breeding has not been reported

Method used

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  • A kind of method of selection chicken stress resistance performance
  • A kind of method of selection chicken stress resistance performance
  • A kind of method of selection chicken stress resistance performance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] 1. Design 2 pairs of primers, as shown in Table 1 below:

[0030] Table 1

[0031]

[0032]

[0033] 2. Using general DNA extraction technology and PCR detection technology, extract chicken blood DNA, use the designed DNA primer sequence, use known LALA and LBLB chicken DNA as a reference, and detect the fragments of sequence 1 and sequence 2 (i.e. C 3 indels in the lysozyme gene).

[0034] 3. Determine the genotype of the C-type lysozyme gene of the individual. Specifically: Electrophoresis detection of PCR amplified fragments. The fragments of the two genes are shown in Table 2. According to the size of the fragments, it is determined that they belong to the LA or LB genotype;

[0035] Table 2

[0036] Gene LA LB PCR product of the first pair of primers 153bp 183bp Second pair of PCR products 153bp 128bp

[0037] In the present invention, the sequence of the LA gene is shown in SEQ ID NO: 7;

[0038] The sequence of the s...

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PUM

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Abstract

The invention discloses a method for selecting chicken resisting performance. The method includes the steps of extracting chicken blood DNA; conducting PCR amplification by means of two primer pairs, wherein the first primer pair is shown in SEQ ID NO:1 and SEQ ID NO:2, and the second primer pair is shown in SEQ IN NO:3 and SEQ IN NO:4; conducting electrophoresis analysis on a PCR amplification segment to determine the specific gene type; selectively weeding out the LALB gene type and keeping the LALA gene type. In the method, chicken of the LALA gene type are high in hatching rate, survival rate and environment accommodative ability.

Description

technical field [0001] The invention belongs to the technical field of breeding industry and relates to chicken breeding technology, in particular to a method for selecting the stress resistance performance of chickens. Background technique [0002] When chickens are attacked by climate change, nutritional factor changes and infectious disease sources, the health and production levels of chickens will decline, and in severe cases, diseases and even death will occur. If the environment of the flock cannot be adjusted in time, or measures such as immunization and treatment are taken in time, the mortality rate of the flock will further increase and the egg production rate will further decrease. The stress resistance of chickens is controlled by the growth and development level and genetics of chickens. In recent years, the survival rate of chickens has been continuously improved, and the survival rate of chickens at 72 weeks without major diseases has increased from 85-90% 10...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888A01K67/027
Inventor 严华祥杨长锁姚峻峰徐志刚李红王家豪
Owner SHANGHAI ACAD OF AGRI SCI