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Scar molecular marker and its identification method for banana b genome identification

A molecular marker and genome technology, applied in the field of plant molecular biology, can solve problems such as difficult to judge, time-consuming, and high cost

Active Publication Date: 2019-12-31
东莞市香蕉蔬菜研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The inventors applied the above four kinds of molecular markers, among which the PCR-RFLP and copia-IRAP markers of ITS need enzyme digestion, which is time-consuming and costly; RAPD markers and gypsy-IRAP markers are complicated due to the large number of bands after amplification. There will be differences in the size of the bands in different bananas, sometimes it is difficult to judge, and the requirements for DNA extraction quality and electrophoresis are relatively high

Method used

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  • Scar molecular marker and its identification method for banana b genome identification
  • Scar molecular marker and its identification method for banana b genome identification
  • Scar molecular marker and its identification method for banana b genome identification

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[0033] specific implementation

[0034]The present invention is further illustrated and defined by the following examples without limitation. Through examples, scientific researchers can have a clearer understanding of the present invention, and can make certain changes and modifications to the present invention on this basis to obtain different research effects. The experimental methods in the following examples are conventional methods unless otherwise specified. The reagents involved in the experiment are all conventional reagents, and they are used according to the product instruction manual.

[0035] 1. DNA extraction from banana leaves

[0036] Banana varieties of different genotypes (Table 1) were selected, and the samples were collected in the Banana Resource Garden of Dongguan Banana Vegetable Research Institute for DNA extraction.

[0037] The DNA extraction method adopts the modified CTAB method, and the specific operation is as follows:

[0038] 1. For the DN...

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Abstract

The invention belongs to the technical field of plant molecular biology and particularly relates to a SCAR molecular marker for banana B genome identification and an identification method thereof and discloses a method capable of identifying whether the banana variety contains B genome. The method comprises the following steps of: amplifying the band by use of an IRAP molecular marker gypsy-IRAP; performing recycling and clone sequencing of the B genome specific band to obtain the sequence thereof; designing a specific primer; and converting the gypsy-IRAP marker into a SCAR molecular marker indicating whether a specific band exists. The method is used for quickly and accurately identifying the B genome of banana and provides good technical support for banana breeding and variety improvement.

Description

technical field [0001] The invention belongs to the technical field of plant molecular biology, and in particular relates to a SCAR molecular marker for banana B genome identification and an identification method thereof. Background technique [0002] Banana (Musa spp.) belongs to Musa family Musa, is one of the most important tropical fruits in the world, and is the fourth largest food crop except rice, wheat and corn (FAO, 1992). my country is one of the main producing countries of banana , has been cultivated for more than two thousand years, and is mainly concentrated in Guangdong, Guangxi, Fujian, Yunnan and other provinces and regions of China. With the rapid development of banana production, the banana industry has become an agricultural pillar industry in tropical regions of my country. [0003] Banana has two ancestors, namely, M. acuminata Colla (recorded as A genome) and long-stemmed banana (M. balbisiana Colla, denoted as B genome). Banana cultivars are evolved ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 王芳吕顺牛玉清夏玲曾莉莎
Owner 东莞市香蕉蔬菜研究所