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Hemibagrus fish interspecific hybridization method

A technology for fish and female fish, which is applied in the field of interspecies hybridization of fishes of the genus Ceruleus, can solve problems such as no public reports, and achieve good body color, strong temperature adaptability, and beneficial effects for breeding

Active Publication Date: 2016-11-09
云南省渔业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Through document retrieval, do not see the public report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for interspecies crossbreeding of fishes of the genus Cichlid, using the wild Cichlid chinensis with a body weight of 200g as the male parent, and the 5+-year-old Cichlid chinensis cultured in ponds as the female parent, specifically through the following steps:

[0022] A. Carry out the following oxytocic injections to the female fish of the silktail fish: the first injection of LRH-A2, the injection volume is 3 μg / kg; the second injection of LRH-A2+DOM, the injection volume of LRH-A2 is 7 μg / kg, The injection volume of DOM is 5 mg / kg; and after the first injection, the second injection is performed at an interval of 12 hours;

[0023] B. At the same time when the female fish of the silktail fish in step A is injected with the second oxytocic agent, the male fish of the blue fish is injected with LRH-A2+DOM, the injection amount of LRH-A2 is 10 μg / kg, and the injection amount of DOM is 5 mg / kg;

[0024] C, Steps A and B have completed the artificial injectio...

Embodiment 2

[0029] A method for interspecies crossbreeding of fishes of the genus Cichlid, using the Culcarus chinensis cultured in ponds with a body weight of 200g as the male parent, and the 5+-year-old Culcaria chinensis cultured in ponds as the female parent, specifically through the following steps:

[0030] A. Carry out the following oxytocic injections to the female fish of the silktail fish: the first injection of LRH-A2, the injection volume is 3 μg / kg; the second injection of LRH-A2+DOM, the injection volume of LRH-A2 is 7 μg / kg, The injection volume of DOM is 5 mg / kg; and after the first injection, the second injection is performed at an interval of 12 hours;

[0031] B. At the same time when the female fish of the silktail fish in step A is injected with the second oxytocic agent, the male fish of the blue fish is injected with LRH-A2+DOM, the injection amount of LRH-A2 is 10 μg / kg, and the injection amount of DOM is 5 mg / kg;

[0032] C, Steps A and B complete the artificial...

Embodiment 3

[0037] A method for interspecies crossbreeding of fishes of the genus Cichlid, using the Culcarus chinensis cultured in ponds with a body weight of 200g as the male parent, and the 5+-year-old Culcaria chinensis cultured in ponds as the female parent, specifically through the following steps:

[0038] A. Carry out the following oxytocic injections to the female fish of the silktail fish: the first injection of LRH-A2, the injection volume is 3 μg / kg; the second injection of LRH-A2+DOM, the injection volume of LRH-A2 is 7 μg / kg, The injection volume of DOM is 5 mg / kg; and after the first injection, the second injection is performed at an interval of 12 hours;

[0039] B. At the same time when the female fish of the silktail fish in step A is injected with the second oxytocic agent, the male fish of the blue fish is injected with LRH-A2+DOM, the injection amount of LRH-A2 is 10 μg / kg, and the injection amount of DOM is 5 mg / kg;

[0040] C, Steps A and B complete the artificial...

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Abstract

The invention relates to a hemibagrus fish interspecific hybridization method and belongs to the technical field of aquatic genetic breeding. Hemibagrus pluriradiatus serves as a male parent, hemibagrus nemurus serves as a female parent, and secondary oxytocic injection, primary LRH-A2 injection and secondary LRH-A2+DOM are conducted on hemibagrus nemurus female fishes; LRH-A2+DOM is injected to hemibagrus pluriradiatus male fishes while secondary oxytocic injection is conducted on the hemibagrus nemurus female fishes, the female fishes and the male fishes are bred in different spawning ponds, water temperatures in the two ponds are kept to be 25-27 DEG C, breeding is conducted for 10-11 hours, artificial egg stripping is conducted on the mature hemibagrus nemurus female fishes, testes of the hemibagrus pluriradiatus male fishes are cut out, sperm is collected, and dry method artificial insemination is conducted; fertilized eggs are conveyed into a flowing water tank and subjected to artificial incubation and fry breeding in flowing water at the temperature of 25-27 DEG C, and hybridized hemibagrus fry are obtained. The obtained hybridized offsprings are good in body color, high in growth speed, high in temperature adaptability and beneficial to breeding.

Description

technical field [0001] The invention relates to a method for interspecies crossbreeding of fishes belonging to the genus Aquatica, which belongs to the technical field of aquatic genetics and breeding. Background technique [0002] Vietchi, scientific name M. Pluriradiatus , the place is called Baixugong and Wansi. Taxonomically, it belongs to Siluriformes Siluriformes, Osmanthus Bagridae, and Osmanthus genus Mystus , distributed in the Red River system. [0003] Silktail, scientific name M. wyckioides , the local name is Bearded Fish. In terms of classification, it belongs to the same genus as Vietna, and it is named because the upper lobe of the caudal fin is extended into filaments. It is an indigenous species of Yunnan in China, distributed in the tributaries of the Lancang River system, rare in the main stream of the Lancang River, and distributed in the Mekong River system abroad. [0004] The meat of the fish of the genus Cystus is tender and snow-white, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 薛晨江薛绍伟马建颜杨明忠
Owner 云南省渔业科学研究院
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