A kind of self-luminous fluorescent microsphere and preparation method thereof

A technology of fluorescent microspheres and self-luminescence, which is applied in the direction of luminescent materials, chemical instruments and methods, etc., can solve the problems affecting the luminescence performance and service life of microspheres, the loss of dyes or quantum dots, and the lack of autofluorescence, etc., to achieve structural Regular, long-lasting luminous effect, not easy to quench

Active Publication Date: 2018-07-17
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, the above types of microspheres do not have the advantage of autofluorescence, and they need to be loaded with dyes or quantum dots to excite fluorescence, which greatly increases the cost.
Moreover, the entrapped dyes or quantum dots will be lost or quenched over time, which greatly affects the luminescence performance and service life of the microspheres.

Method used

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  • A kind of self-luminous fluorescent microsphere and preparation method thereof
  • A kind of self-luminous fluorescent microsphere and preparation method thereof
  • A kind of self-luminous fluorescent microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Add 1.0 g of phenol, 5 mL of formaldehyde and 40 mL of distilled water into a three-necked flask, mix them uniformly by ultrasonic, stir at 250 r / min, and raise the temperature to 80 o C, keep warm for 30 min.

[0028] (2) Add 4 mL of ammonia water to the pre-reaction solution obtained in step (1). After 24 h of reaction, add 1 mol / L hydrochloric acid solution to adjust the pH to neutral, and cool the reaction system to room temperature.

[0029] (3) The product of step (2) was centrifuged at 5000 r / min, washed three times with distilled water, ethanol, and distilled water, and finally dried in a vacuum oven for 4 h.

[0030] The particle size of the prepared self-luminous fluorescent microspheres was 4.5 µm.

Embodiment 2

[0032] (1) Add 1.0 g of phenol, 5 mL of formaldehyde and 40 mL of distilled water into a three-necked flask, mix them uniformly by ultrasonic, stir at 250 r / min, and raise the temperature to 80 o C, keep warm for 30 min.

[0033] (2) Add 6 mL of ammonia water to the pre-reaction solution obtained in step (1). After 24 h of reaction, add 1 mol / L hydrochloric acid solution to adjust the pH to neutral, and cool the reaction system to room temperature.

[0034] (3) The product of step (2) was centrifuged at 5000 r / min, washed three times with distilled water, ethanol, and distilled water, and finally dried in a vacuum oven for 4 h.

[0035] The particle size of the prepared self-luminous fluorescent microspheres was 3 µm.

Embodiment 3

[0037] (1) Add 1.0 g of phenol, 5 mL of formaldehyde and 40 mL of distilled water into a three-necked flask, mix them uniformly by ultrasonic, stir at 250 r / min, and raise the temperature to 80 o C, keep warm for 30 min.

[0038] (2) Add 8 mL of ammonia water to the pre-reaction solution obtained in step (1). After 24 h of reaction, add 1 mol / L hydrochloric acid solution to adjust the pH to neutral, and cool the reaction system to room temperature.

[0039] (3) The product of step (2) was centrifuged at 5000 r / min, washed three times with distilled water, ethanol, and distilled water, and finally dried in a vacuum oven for 4 h.

[0040] The particle size of the prepared self-luminous fluorescent microspheres was 2.5 µm.

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Abstract

The invention discloses a self-luminous fluorescent microsphere and a preparation method thereof, which belong to the technical field of a functional material. According to the invention, a modified St-ber method is employed, phenol, formaldehyde or glutaraldehyde are subjected to a sol-gel reaction to prepare the fluorescent microsphere having self-luminous characteristic, and the fluorescence has the advantages of lasting luminescence and difficult quenching. The used preparation method is simple, no surfactant and emulsifier is required, and phenol and formaldehyde (or glutaraldehyde) are reacted in water under ammoniacal liquor catalysis at low temperature (the reaction temperature is no higher than 95 DEG C). The prepared fluorescent microsphere has the advantages of controllable particle size, uniform distribution, monodispersion, self-luminous characteristic, lasting luminescence and difficult quenching. The self-luminous fluorescent microsphere can be stably dispersed in water, ethanol, acetone, and the solutions of dimethylformamide, and acetonitrile, and has wide application prospect in the fields of fluorescent probe microspheres and flow-type intracellular biological analysis.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a self-luminous fluorescent microsphere and a preparation method thereof. Background technique [0002] Polymer fluorescent microspheres refer to polymer microspheres with diameters ranging from nanometers to micrometers and loaded with fluorescent substances. Its shape can be any shape, generally spherical. Among them, fluorescent microspheres have shown great application potential in the fields of labeling, tracing, detection, standards, immobilized enzymes, immunomedicine, and high-throughput drug screening due to their stable morphological structure and stable and efficient luminous efficiency. Therefore, in recent years, the research on high-performance polymer fluorescent microspheres has attracted more and more attention from domestic and foreign scientists, and has become a hot and difficult research topic in this field. [0003] Polymer fluores...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G8/10C08G8/04C09K11/06
CPCC08G8/04C08G8/10C09K11/06C09K2211/1425
Inventor 游力军宋立岛王傲张其清
Owner FUZHOU UNIV
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