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Gerbera jamesonii seedling transplanting and hardening substrate and application thereof

A seedling hardening substrate and seedling hardening technology, applied in planting substrate, application, cultivation, etc., can solve the problems of unsatisfactory transplanting survival rate of tissue cultured seedlings, poor adaptability, and low survival rate of tissue cultured seedlings transplanted

Inactive Publication Date: 2016-11-16
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tissue cultured seedlings produced in the tissue culture room have poor adaptability to the outside world, and are likely to cause weak seedlings and dead seedlings. Therefore, the transplanted survival rate of tissue cultured seedlings is not high, and some are even lower than 50%, resulting in great waste of resources
From the tissue culture room to the outdoor cutting stage must go through the seedling hardening program, the seedling hardening matrix is ​​the most important part, the gerbera seedling hardening matrix of the prior art is not ideal for the transplanting survival rate of the tissue culture seedlings, in terms of water retention capacity and Seedling nutrition needs to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A method for cultivating gerbera detoxified seedlings based on transplanting seedling hardening matrix, the composition of said seedling hardening matrix is: 50g of perlite, 50g of vermiculite, 25g of sawdust, 25g of pine bark, 15g of water moss, 5g of flower mud, Peat soil 5g and calcium magnesium phosphorus potassium fertilizer 5g.

[0051] The specific steps of the cultivation method are as follows:

[0052] (1) Receptacle explant pretreatment:

[0053] Select flower buds with a diameter of 1-2 cm, refrigerate them at 4°C for 3 days, take them out and return them to room temperature, peel off the bracts and florets, wash them with pure water, and sterilize them in 0.05% mercuric chloride aqueous solution for 5 minutes, then Disinfect with 70% alcohol for 30 seconds, and then rinse with sterile water for 3 times to obtain the gerbera receptacle explant.

[0054] (2) Explant inoculation and induction culture:

[0055] Transfer the gerbera receptacle explants into th...

Embodiment 2

[0064] A method for preserving gerbera germplasm resources based on transplanting seedling hardening matrix, the composition of said seedling hardening matrix is: perlite 100g, vermiculite 150g, sawdust 80g, pine bark 80g, sphagnum moss 50g, flower mud 15g, Peat soil 15g and calcium magnesium phosphorus potassium fertilizer 1.5g, the concrete steps of described germplasm resource preservation method are as follows:

[0065] (1) Receptacle explant pretreatment:

[0066] Select flower buds with a diameter of 2 cm, refrigerate them at 4°C for 5 days, take them out and return them to room temperature naturally, peel off the bracts and florets, wash them with pure water, sterilize them in 0.05% mercuric chloride aqueous solution for 8 minutes, and then use 70 % alcohol for 40 seconds, and then rinsed with sterile water for 3 times to obtain the Gerbera chrysanthemum receptacle explants.

[0067] The percentages mentioned above are volume percentages.

[0068] (2) Explant inoculat...

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PUM

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Abstract

The invention discloses a gerbera jamesonii seedling transplanting and hardening substrate. The substrate is prepared from perlite, vermiculite, sawdust, pine bark, a pericarpium citri reticulatae alcohol extract, a folium artemisiae argyi alcohol extract, sphagna, flower mud, peat soil and calcium magnesium potassium phosphate fertilizer. The gerbera jamesonii seedling transplanting and hardening substrate can be applied to gerbera jamesonii tissue culture and rapid propagation and gerbera jamesonii germplasm in-vitro preservation. The gerbera jamesonii seedling transplanting and hardening substrate significantly increases the transplant survival rate of gerbera jamesonii seedlings, has the technical effect of water and fertilizer retention and has an application prospect in the plant cultivation field.

Description

technical field [0001] The invention relates to a substrate for transplanting seedlings, in particular to a substrate for transplanting seedlings of gerbera species and its application in the field of tissue culture and rapid propagation of gerbera. Background technique [0002] Gerbera is a perennial evergreen herbaceous flower of the genus Gerbera in the family Asteraceae. It is one of the top five cut flowers in the world. It is popular among people because of its large flowers, bright flowers, full color and long vase life. It is widely planted in my country. [0003] Since most gerbera are self-infertile, hybrid offspring will split and mutate. When genetic information is passed from parent to offspring, the variety of gerbera will degenerate and lose its original typical due to the cultivation environment such as light, water, temperature, nutrition and other reasons. It usually manifests as smaller flowers, more transitional colors, disordered flower shape, thinner fl...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01H4/00A01G9/10C05G3/00
CPCA01G24/00A01G22/00A01H4/008C05D9/00C05G3/00C05F11/00C05F11/02C05G1/00
Inventor 许建中孟剑华林少会
Owner WUXI NANLIGONG TECH DEV
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