The invention discloses a preparation process of child safety crystal mud with an antibacterial function, which comprises the following steps: adding deionized water, a plant humectant, a food-grade thickener and table vinegar into a reaction kettle, stirring the components until the components are completely mixed, and heating the mixture; cooling the mixture to a certain temperature, and addinga natural preservative to obtain a solution; performing heat preservation, then adding edible gum, edible glycerol, starch, edible color paste, garlic powder, onion powder, honey and traditional Chinese medicine mixed powder, and stirring the mixture for 3-4 hours; standing the crystal mud for defoaming, placing the crystal mud into a refrigerated container at 0 DEG C for 3 hours, and moving out and fill-packaging the crystal mud. The crystal mud prepared by the process has a good antibacterial function and can well prevent pathogenic bacteria infection of children during playing, so that theplaying safety of the crystal mud is improved.