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Temperature-sensitive composition for treating periodontal diseases and preparation method of temperature-sensitive composition

A technique for periodontal diseases and compositions, which is applied in the field of temperature-sensitive compositions for the treatment of periodontal diseases and its preparation, can solve the problems of poor tissue adhesion, disappearance, and decreased hydrophilicity, so as to improve the treatment effect and reduce the The effect of using and prolonging the action time

Active Publication Date: 2020-07-31
诺一迈尔(山东)医学科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used temperature-sensitive materials such as poly(N-isopropylacrylamide) (PNIPAM) and hydroxypropyl methylcellulose, etc., when the temperature rises, the hydrophilic groups in the molecules of these temperature-sensitive materials interact with water The hydrogen bonds between the molecules are weakened, resulting in a decrease in hydrophilicity, and the gel becomes hydrophobic and gradually shrinks. Although the gel strength increases, its viscosity decreases or even disappears. Therefore, this type of temperature-sensitive gel Glue has poor tissue adhesion

Method used

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  • Temperature-sensitive composition for treating periodontal diseases and preparation method of temperature-sensitive composition
  • Temperature-sensitive composition for treating periodontal diseases and preparation method of temperature-sensitive composition
  • Temperature-sensitive composition for treating periodontal diseases and preparation method of temperature-sensitive composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Preparation of polylysine-modified hyaluronic acid

[0037] 379 mg of hyaluronic acid with a molecular weight of 1,500,000 was dissolved in 80 ml of deionized water, 395.4 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), 230.1 mg of N- Hydroxysuccinimide (NHS) was slowly added to the aqueous solution of hyaluronic acid, stirred evenly for 30 min, and then 128 mg of ε-polylysine with a molecular weight of 3600-4300 (pre-dissolved in 20 mL of deionized water), the reaction process maintained the pH between 4-5, and after 3 hours of reaction, the reaction was continued at room temperature for 12 hours. After the reaction, the solution was purified by dialysis in deionized water with pH=5 for 96 h. The dialysis-completed product was pre-frozen at -80°C for 12 hours, and then placed in a vacuum freeze dryer for freeze-drying for 72 hours until the water was completely drained to obtain polylysine-modified hyaluronic acid for future us...

Embodiment 2

[0039] Embodiment 2: the preparation of hydroxypropyl chitin

[0040] Disperse 6g of chitin powder evenly in 74g of 40% NaOH aqueous solution at low temperature, stir overnight at room temperature, add 220g of ice cubes and stir at room temperature until the solution is clear and transparent, and finally obtain a 2wt% chitin solution for use.

[0041] Weigh 100g of 2wt% chitin solution, add 24g of 1,2-propylene oxide to the chitin solution at low temperature, stir for 12 hours at low temperature, heat up to 25°C and stir for 10h, then add hydrochloric acid solution to adjust the pH to neutral to terminate the reaction, After the reaction product was dialyzed for 72 hours, it was lyophilized to obtain white powdery hydroxypropyl chitin (the degree of substitution of the hydroxypropyl group in the hydroxypropyl chitin was 1.25) for use.

[0042] Other methods known in the art for preparing hydroxypropyl chitin can also be used in the present invention.

Embodiment 3

[0043] Example 3: Preparation of temperature-sensitive composition formulations for the treatment of periodontal diseases

[0044] The thermosensitive composition of the present embodiment adopts following method to prepare:

[0045] (1) The hydroxypropyl chitin prepared in Example 2 is dispersed in water to obtain an aqueous solution of hydroxypropyl chitin;

[0046] (2) adding sodium carboxymethylcellulose to the aqueous solution of hydroxypropyl chitin obtained in step (1) to obtain a mixed solution;

[0047] (3) The polylysine-modified hyaluronic acid prepared in Example 1 was formulated into an aqueous dispersion liquid and added to the mixed solution obtained in step (2) for stirring to obtain a temperature-sensitive composition.

[0048] Temperature-sensitive compositions A and B were respectively obtained by adopting different formulations by the above method.

[0049] The temperature-sensitive composition A includes: the polylysine-modified hyaluronic acid prepare...

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Abstract

The present invention provides a temperature-sensitive composition for treating periodontal diseases. The temperature-sensitive composition is prepared from an aqueous medium, sodium carboxymethyl cellulose, a therapeutically effective amount of polylysine-modified hyaluronic acid or a pharmaceutically acceptable salt thereof, and a temperature-sensitive material, namely bydroxylpropyl chitin in which the degree of substitution of hydroxypropyl for chitin is 1.0 to 1.5, wherein the amount of the sodium carboxymethyl cellulose in the temperature-sensitive composition is enough to enable the temperature-sensitive composition to have the viscosity of 10 Pa*s to 40 Pa*s at the room temperature. The temperature-sensitive composition is in a viscous solution state before being applied to the interior of the oral cavity, can quickly form a gel state when being applied to the interior of the oral cavity, and can adhere to an application site well, so that the action time of the active component, namely the polylysine-modified hyaluronic acid, in gel on an affected part is prolonged, and the treatment effect is further improved; and meanwhile, the active component, namely the polylysine-modified hyaluronic acid, in the temperature-sensitive composition has the antibacterial property while the physiological activity of the hyaluronic acid is retained.

Description

technical field [0001] The invention relates to the field of medical biomaterials, in particular to a temperature-sensitive composition for treating periodontal diseases and a preparation method thereof. Background technique [0002] Oral health is an integral part of overall health. According to the results of the "Fourth National Oral Epidemiological Survey" released by the former National Health and Family Planning Commission in 2017, the periodontal health rate of Chinese adults is only 9.1%, and periodontal disease It is a common oral disease, is one of the main causes of adult tooth loss, and is also a major oral disease that endangers human teeth and general health. Periodontal disease refers to chronic, nonspecific, infectious diseases that occur in tooth supporting tissues (including gingiva, periodontal ligament, alveolar bone and cementum). According to the site of disease invasion, it is divided into two categories: gingivitis and periodontitis. [0003] Hyalur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14A61L31/16
CPCA61L31/041A61L31/16A61L31/145A61L31/14C08L1/286C08L5/08
Inventor 郭刚余建均李冲刘泽汉董彦君顾金花
Owner 诺一迈尔(山东)医学科技有限公司
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