Serum-free insect cell culture medium and preparation method and application thereof

Abstract

The invention provides a serum-free insect cell culture medium and a preparation method and application thereof. The serum-free insect cell culture medium is prepared from basic components, protein components, lipid components and auxiliaries, wherein the basic components include, by weight, 9-14 parts of inorganic salt, 3.3-5 parts of amino acid or salt, 0.17-0.26 part of water soluble vitamins, 0.0016-0.0024 part of hormones and 0.0024-0.0036 part of microelements; the protein components include, by weight, 1.6-2.4 parts of animal protein hydrolysate, 2.4-3.6 parts of plant protein hydrolysate and 2.4-3.6 parts of a yeast extract; the lipid components include, by weight, 0.14-0.22 part of fatty acid, 0.028-0.042 part of lipid-soluble vitamins and 0.24-0.36 part of cholesterol; the auxiliaries include, by weight, 7.2-10.8 parts of a defoaming agent and 2.6-4 parts of a surface active agent. The serum-free insect cell culture medium is low in cost, good in long-term storage stability, high in cell density during insect cell culture and capable of achieving large-scale production and application easily.

Description

technical field [0001] The invention relates to an insect cell culture medium, in particular to a serum-free insect cell culture medium and its preparation method and application. Background technique [0002] Insect cell culture is a rapidly developing field in cell engineering in recent years, and it is widely used in various aspects of medicine, agriculture and biology. Insect cell culture medium is the key to insect cell culture, and it is an important factor for the growth, proliferation and differentiation of insect cells. Insect cells have high nutritional requirements and often need a variety of amino acids, vitamins, coenzymes, nucleic acids, hormones, growth factors, etc. [0003] Natural culture medium is mainly obtained from body fluids or isolated from animal tissues. It is rich in nutrients and has a good culture effect. Common ones include hydrolyzed milk protein and newborn bovine serum. Although serum is more effective for cell growth, there are many disad...

AI Technical Summary

Problems solved by technology

Although serum is more effective for cell growth, there are many disadvantages in its application: high-quality animal serum has limited sources and high cost, which limits its mass use; animal serum has complex components, and various biological and large molecules are mixed together. It is not clear so far, and it will cause certain difficulties in the separation, purification and detection of later culture products; serum is also an important source of mycoplasma and other virus contamination; the quality of each batch of serum is different, which affects the stability of cell growth and replication. It is impossible to use serum medium for large-scale commercial production and application

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