Serum-free insect cell culture medium and preparation method and application thereof

A technology of insect cells and culture medium, applied in the field of serum-free insect cell culture medium and its preparation, can solve the problems of commercial production and application of serum medium, difficulties in separation, purification and detection of cultured products, and influence on cell growth and replication Stability and other issues, to achieve the effect of stable cell growth state, wide application prospects, and high yield of target products

Inactive Publication Date: 2016-11-23
中生天信和(无锡)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although serum is more effective for cell growth, there are many disadvantages in its application: high-quality animal serum has limited sources and high cost, which limits its mass use; animal serum has complex components, and various biological and large molecules are mixed together. It is not clear so far, and it will cause certain difficulties

Method used

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  • Serum-free insect cell culture medium and preparation method and application thereof
  • Serum-free insect cell culture medium and preparation method and application thereof
  • Serum-free insect cell culture medium and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The serum-free insect cell culture medium of this embodiment consists of basic components, protein components, lipid components and auxiliary agents, wherein the basic components are composed of inorganic salts, amino acids or their salts, water-soluble vitamins, trace elements and 0.002 The insulin (hormone) of weight part is made up of, and protein component is made up of 2 weight parts of meat peptone, 3 weight parts of soybean protein hydrolyzate and the yeast extract of 3 weight parts, and lipid component is made up of fatty acid, fat-soluble vitamin and 0.3 The cholesterol of weight part is formed, and auxiliary agent is made up of the Tween 80 (surfactant) of poloxamer 188 (defoamer) of 9 weight parts and 3.3 weight parts; Wherein:

[0057] Inorganic salt is made up of following composition by weight:

[0058]

[0059] Amino acids or their salts consist of the following ingredients in parts by weight:

[0060]

[0061]

[0062] Water-soluble vitamins co...

Embodiment 2

[0079] The serum-free insect cell culture medium of this embodiment consists of basic components, protein components, lipid components and auxiliary agents, wherein the basic components are composed of inorganic salts, amino acids or their salts, water-soluble vitamins, trace elements and 0.0016 The insulin of weight part is made up of, and protein component is made up of the tryptone of 1.6 weight part, the wheat protein hydrolyzate of 2.4 weight part and the yeast extract of 3.6 weight part, and lipid component is made up of fatty acid, fat-soluble vitamin and 0.36 weight part of Cholesterol composition, auxiliary agent is made up of the poloxamer 188 of 7.2 weight parts and the Tween 80 of 4 weight parts; Wherein:

[0080] Inorganic salt is made up of following composition by weight:

[0081]

[0082] Amino acids or their salts consist of the following ingredients in parts by weight:

[0083]

[0084]

[0085] Water-soluble vitamins consist of the following compon...

Embodiment 3

[0097] The serum-free insect cell culture medium of this embodiment consists of basic components, protein components, lipid components and auxiliary agents, wherein the basic components are composed of inorganic salts, amino acids or their salts, water-soluble vitamins, trace elements and 0.0024 The insulin of weight part is made up of, and protein component is made up of the bone peptone of 2.4 weight parts, the potato protein hydrolyzate of 3.6 weight parts and the yeast extract of 2.4 weight parts, and lipid component is made up of fatty acid, fat-soluble vitamin and 0.24 weight part of Cholesterol composition, auxiliary agent is made up of the poloxamer 188 of 10.8 weight parts and the Tween 80 of 2.6 weight parts; Wherein:

[0098] Inorganic salt is made up of following composition by weight:

[0099]

[0100] Amino acids or their salts consist of the following ingredients in parts by weight:

[0101]

[0102]

[0103] Water-soluble vitamins consist of the follo...

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Abstract

The invention provides a serum-free insect cell culture medium and a preparation method and application thereof. The serum-free insect cell culture medium is prepared from basic components, protein components, lipid components and auxiliaries, wherein the basic components include, by weight, 9-14 parts of inorganic salt, 3.3-5 parts of amino acid or salt, 0.17-0.26 part of water soluble vitamins, 0.0016-0.0024 part of hormones and 0.0024-0.0036 part of microelements; the protein components include, by weight, 1.6-2.4 parts of animal protein hydrolysate, 2.4-3.6 parts of plant protein hydrolysate and 2.4-3.6 parts of a yeast extract; the lipid components include, by weight, 0.14-0.22 part of fatty acid, 0.028-0.042 part of lipid-soluble vitamins and 0.24-0.36 part of cholesterol; the auxiliaries include, by weight, 7.2-10.8 parts of a defoaming agent and 2.6-4 parts of a surface active agent. The serum-free insect cell culture medium is low in cost, good in long-term storage stability, high in cell density during insect cell culture and capable of achieving large-scale production and application easily.

Description

technical field [0001] The invention relates to an insect cell culture medium, in particular to a serum-free insect cell culture medium and its preparation method and application. Background technique [0002] Insect cell culture is a rapidly developing field in cell engineering in recent years, and it is widely used in various aspects of medicine, agriculture and biology. Insect cell culture medium is the key to insect cell culture, and it is an important factor for the growth, proliferation and differentiation of insect cells. Insect cells have high nutritional requirements and often need a variety of amino acids, vitamins, coenzymes, nucleic acids, hormones, growth factors, etc. [0003] Natural culture medium is mainly obtained from body fluids or isolated from animal tissues. It is rich in nutrients and has a good culture effect. Common ones include hydrolyzed milk protein and newborn bovine serum. Although serum is more effective for cell growth, there are many disad...

Claims

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Application Information

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IPC IPC(8): C12N5/07
Inventor 张宇王建超赵洪磊徐舸辰
Owner 中生天信和(无锡)生物科技有限公司
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