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Multi-chamber culture vessel and cell culturing method

A culture container and cell culture technology, which is applied to cell culture active agents, tissue culture, animal cells, etc., and can solve problems such as pollution, complicated disposal, and solution overflow

Active Publication Date: 2016-11-30
TOYO SEIKAN GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when transferring such cells in an open system, there is a risk of contamination, so in the case of transferring cells by manual operation, it is necessary to operate in a sterile environment
In addition, when the cells are transferred between multiple containers, the solution may overflow, and the relationship between the containers must be strictly managed, and there is a problem of complicated handling.

Method used

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  • Multi-chamber culture vessel and cell culturing method
  • Multi-chamber culture vessel and cell culturing method
  • Multi-chamber culture vessel and cell culturing method

Examples

Experimental program
Comparison scheme
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no. 1 approach

[0032] First, refer to figure 1 , the multi-chamber culture container and the cell culture method according to the first embodiment of the present invention will be described.

[0033] The multi-chamber culture container of this embodiment is characterized in that it is a multi-chamber culture container obtained by processing gas-permeable materials, and two or more culture chambers for culturing cells are formed, and each culture chamber is connected to at least one culture chamber. The other culture chambers communicate with each other and have at least one external connection for communicating with the outside of the multi-chamber culture container.

[0034] in particular, figure 1 Among them, the multi-chamber culture container 1 includes a culture chamber 11 and a culture chamber 12 as a culture area for culturing cells.

[0035] The culture chamber 11 is provided with an external connection port 111 for communicating with the outside of the multi-chamber culture contai...

no. 2 approach

[0062] Below, refer to figure 2 , the multi-chamber culture container and the cell culture method according to the second embodiment of the present invention will be described.

[0063] The multi-chamber culture container 1 a of the present embodiment includes a culture chamber 13 in addition to the culture chamber 11 and the culture chamber 12 as shown in the figure.

[0064] The culture chamber 13 is provided with an external connection port 131 for communicating with the outside of the multi-chamber culture container 1a, and an inter-chamber connection port 132 for communicating with another culture chamber.

[0065] Although not shown, the external connection port 131 is connected to a cell supply container or the like via the line 2 and used for injecting cells into the culture chamber 13 or the like. The inter-chamber connection port 132 is connected to the inter-chamber connection port 112 of the culture chamber 11 and the inter-chamber connection port 122 of the cult...

no. 3 approach

[0079] Below, refer to image 3 and Figure 4 , the multi-chamber culture container and the cell culture method according to the third embodiment of the present invention will be described.

[0080] The multi-chamber culture container of this embodiment is characterized in that it is equipped with two or more inter-chamber connection parts connected by pipelines on one side, so that the side of each culture chamber provided with the inter-chamber connection part is connected to the multi-chamber culture container. Each culture chamber is formed in such a way that the distance between the corresponding sides of the chamber becomes larger or smaller according to the arrangement order of each culture chamber. In other respects, the same configuration as that of the multi-chamber culture container 1 of the first embodiment may be employed.

[0081] Specifically, as image 3 As shown, the distance d1 between the side of the multi-chamber culture container 1b equipped with the in...

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PUM

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Abstract

The purpose of the present invention is to provide a culture vessel with which cells that have been cultured under a plurality of different culture conditions can be transferred by simple work. A multi-chamber culture vessel (1) obtained by processing a material that is permeable to gas, wherein two or more culture chambers for culturing cells are formed, each of the culture chambers (11, 12) communicating with at least one other culture chamber, and being provided with at least one of external connection sections (111, 121) that communicate with the exterior of the multi-chamber culture vessel.

Description

technical field [0001] The invention relates to a cell culture technology, in particular to a cell culture container capable of culturing cells in multiple different culture environments and culture conditions. Background technique [0002] In recent years, in fields such as the production of pharmaceuticals, gene therapy, regenerative medicine, and immunotherapy, it is required to efficiently culture cells and tissues in large quantities in an artificial environment. [0003] In the process of culturing cells, tissues, etc., a plurality of different culture environments and culture conditions are sometimes required. In addition, cells cultured under a plurality of different culture environments and culture conditions may also be mixed in a later step. In these cases, cultured cells need to be transferred between multiple vessels. [0004] However, when such a cell transfer is performed in an open system, there is a risk of contamination. Therefore, when the cell transfer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/071C12N5/0783
CPCC12M23/14C12M23/34C12M29/00C12M35/08C12M41/00C12M23/24C12N5/0634C12N5/0635C12N5/0636C12N5/0639C12M23/02C12N2501/515C12M29/26
Inventor 田中乡史户谷贵彦
Owner TOYO SEIKAN GRP HLDG LTD
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