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Multi-sample culture device

A culture device and multi-sample technology, applied in the field of cell culture, can solve problems such as lack, and achieve the effects of high accuracy, reasonable space layout, and convenient operation process

Inactive Publication Date: 2016-12-07
刘力伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, when conducting cell culture and comparison tests in the process, although ordinary culture devices can already provide the carrying space for the culture medium, they are not ideal in terms of reasonable space layout, overall operation convenience, and consistency of control effects. , still lacking

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0044] Example 1: In the circumferential direction of the rotating tube, the drain groove, the liquid equalizing hole, the tube liquid supply hole, and the cylinder liquid supply hole are sequentially arranged in a clockwise direction. During the operation of liquid equalization control (that is, the aforementioned process of draining liquid through the liquid equalization hole to make the liquid volume in each dish consistent), the liquid drainage tank rotates clockwise with the rotating tube to align with the liquid equalization hole (during this process, the tube feeds liquid hole is not connected to the liquid supply hole of the corresponding tube), after the liquid equalization control is completed, the liquid discharge tank continues to rotate clockwise and is staggered from the liquid equalization hole. At this time, the liquid supply hole of the tube and the liquid supply hole of the corresponding cylinder are still not connected. Then, if you continue to rotate the rot...

example 2

[0045] Example 2: In the circumferential direction of the rotating tube, the uniform liquid hole, the liquid drainage groove, the liquid supply hole of the tube, and the liquid supply hole of the cylinder are arranged in a clockwise direction, and the liquid is controlled (that is, the liquid is drained through the liquid uniform hole as described above) The process of making the liquid volume in each dish consistent) During operation, the liquid discharge tank rotates counterclockwise with the rotating tube to align with the liquid equalization hole (during this process, the liquid supply hole of the tube is not connected to the liquid supply hole of the corresponding cylinder), and the liquid control volume After the completion, the liquid discharge tank rotates clockwise and is staggered with the liquid equalizing hole. At this time, the liquid supply hole of the tube and the liquid supply hole of the corresponding cylinder are still not connected. Then, if you continue to ro...

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Abstract

The invention discloses a multi-sample culture device which comprises a base and a plurality of culture vessels. All the culture vessels are stacked, each culture vessel comprises a vessel body, a vertical barrel, a protruding block and a liquid injection pipe, a vessel top groove is formed in the top end face of each vessel body, a base top groove is formed in the top of the base, and the protruding block of the bottommost culture vessel is located in the base top groove. A rotary pipe penetrates through all the vertical barrels, the rotary pipe and the vertical barrels can relatively rotate, the outer side wall of the rotary pipe and the inner side walls of the vertical barrels are in sealed contact, and a liquid drainage groove is formed in the outer side wall of the rotary pipe. A liquid balancing hole communicated with the inside of the corresponding vessel body is formed in each vertical barrel, a cell filter screen and a liquid balancing one-way valve are arranged in each liquid balancing hole, and the lower end of each liquid injection pipe is communicated with the inside of the corresponding vessel body. The protruding blocks of the upper culture vessels are located in the vessel top grooves of the lower culture vessels. The multi-sample culture device has the advantages of being suitable for cell culture, testing and comparing, reasonable in spatial arrangement, stable in structure, convenient to operate, good in adjusting consistence in the experiment process and high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a multi-sample culture device. Background technique [0002] Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. The growth of cells requires a nutrient environment, and the nutrient matrix used to maintain cell growth is called a medium. The medium can be divided into liquid medium and solid medium according to its physical state. In addition to being used in production, cell culture technology is currently used more in experiments (such as determining the parameters of group samples, and conducting comparisons and research), and liquid culture media is commonly used in experiments. In addition, cell culture requires the use of cell culture devices, such as petri dishes, incubators, etc. Cell culture in general environments can directly use petri dishes, and if some special environments (such as power...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06
CPCC12M23/38C12M23/42C12M23/46C12M23/48C12M29/00C12M29/04C12M33/04
Inventor 王铁苗
Owner 刘力伟
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