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Modified plants

A plant and nucleic acid technology, applied in plant gene improvement, plant peptides, plant products, etc., can solve problems such as increased Pi intake in Arabidopsis

Inactive Publication Date: 2016-12-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Thus, although increased PHF activity resulted in PHT translocation from ER to PM, this did not result in increased Pi uptake in Arabidopsis

Method used

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Embodiment Construction

[0053] The present invention provides plants having surprisingly increased Pi uptake without causing symptoms of Pi intoxication. The plant is a mutant plant expressing a PT gene encoding a mutant PT polypeptide having a point mutation at a conserved phosphorylation site. As described herein, these plants showed increased Pi uptake even under low Pi conditions. Also surprisingly, Pi uptake was not increased when wild-type (wt) PT was overexpressed under these conditions. While such overexpression increased the mass of the PT protein, under low Pi conditions, increased expression of the wt protein did not lead to increased Pi uptake and yield. Only overexpression of PT non-phosphorylation mutants with mutations in one of the conserved phosphorylation sites on serine (S) residue corresponding to 517 of OsPT8 resulted in increased Pi uptake. Modification of other phosphorylation sites did not lead to increased Pi uptake and yield.

[0054] Importantly, the inventors showed tha...

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Abstract

Disclosed are improved plants that have increased yield. The plants show increased yield under low phosphate conditions and therefore require less fertilizer. The plants are characterised by expression of a mutant phosphate transporter gene.

Description

technical field [0001] The invention relates to a transgenic monocot plant expressing a nucleic acid structure. Background technique [0002] The extensive use of phosphorus fertilizers in agriculture to maintain high yields has led to serious environmental problems, and it is expected that non-regenerative Pi will be used up within 70 to 200 years (1,2), thus making the essential plant macronutrient phosphate (essentialplant macronutrient phosphate, Pi) has received more and more attention. Therefore, improving plant Pi utilization has become an important goal of sustainable agricultural production. [0003] Phosphorus is an essential nutrient element for plant growth and development. Pi-deficient plants often turn dark green and appear stunted. Plants acquire Pi directly from their environment through the active uptake of Pi by Pi transporters into the epidermal and cortical cells of their roots. After entering root superficial cells, Pi must eventually enter the aplas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415Y02A40/146C12N15/8261G01N33/68G01N2333/415G01N2440/14
Inventor P.吴J.陈
Owner ZHEJIANG UNIV
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