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Doxycycline broad-spectrum monoclonal antibody and preparation technology

A technology of monoclonal antibody and doxycycline, applied in the preparation method of peptide, anti-bacterial immunoglobulin, organic chemistry, etc., can solve the problems of consumers' physical and mental health hazards, economic losses, drug-resistant bacteria, etc., and achieve excellent biological performance effect

Inactive Publication Date: 2016-12-21
陕西思尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Doxycycline is one of the important members of tetracycline antibiotics. It is widely used clinically in animal husbandry and veterinary. The accumulation of residues in feeding tissues not only leads to the emergence of drug-resistant bacteria, but also seriously affects the export of animal husbandry products in my country, causing major economic losses, and also brings great harm to the physical and mental health of consumers. In addition, breeders also There are medication regulations that do not comply with the drug dosage, drug administration site, and drug animal species, as well as the phenomenon of repeated use of several drugs with different trade names but the same ingredients. Therefore, it is necessary to strengthen the monitoring of veterinary drug residues in food of animal origin

Method used

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  • Doxycycline broad-spectrum monoclonal antibody and preparation technology
  • Doxycycline broad-spectrum monoclonal antibody and preparation technology
  • Doxycycline broad-spectrum monoclonal antibody and preparation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A doxycycline broad-spectrum monoclonal antibody is made from the following raw materials in parts by weight:

[0029] 40 parts of doxycycline, 4 parts of tetracycline, 2 parts of minocycline, 2 parts of metacycline, 3 parts of bovine serum albumin, 5 parts of Freund's complete adjuvant, 1 part of Freund's incomplete adjuvant, HRP- 2 parts of goat anti-mouse IgG, 3 parts of ammonium sulfate, 9 parts of disodium hydrogen phosphate, 16 parts of potassium dihydrogen phosphate, 12 parts of citric acid, 8 parts of ammonium dihydrogen phosphate, 9 parts of absolute ethanol, 1 part of isobutyl chloroformate 2 parts of coating buffer, 5 parts of blocking solution, 3 parts of washing buffer, 6 parts of substrate, and 20 parts of cell culture medium.

[0030] The coating buffer is selected from a carbonate buffer with a concentration of 0.05mol / L and a pH value of 9.6; the blocking solution uses 2g of imported gelatin, which is obtained by adding 100ml of coating buffer to boilin...

Embodiment 2

[0040] A doxycycline broad-spectrum monoclonal antibody is made from the following raw materials in parts by weight:

[0041] 46 parts of doxycycline, 12 parts of tetracycline, 4 parts of minocycline, 3 parts of metacycline, 4 parts of bovine serum albumin, 6.5 parts of Freund's complete adjuvant, 1.5 parts of Freund's incomplete adjuvant, HRP- 2.5 parts of goat anti-mouse IgG, 4.5 parts of ammonium sulfate, 9.5 parts of disodium hydrogen phosphate, 18 parts of potassium dihydrogen phosphate, 14 parts of citric acid, 10 parts of ammonium dihydrogen phosphate, 12 parts of absolute ethanol, 2 parts of isobutyl chloroformate 3 parts of coating buffer, 6 parts of blocking solution, 3.5 parts of washing buffer, 8 parts of substrate, and 22.5 parts of cell culture medium.

[0042] The coating buffer is selected from a carbonate buffer with a concentration of 0.05mol / L and a pH value of 9.6; the blocking solution uses 2g of imported gelatin, which is obtained by adding 100ml of coati...

Embodiment 3

[0052] A doxycycline broad-spectrum monoclonal antibody is made from the following raw materials in parts by weight:

[0053]52 parts of doxycycline, 20 parts of tetracycline, 6 parts of minocycline, 4 parts of metacycline, 5 parts of bovine serum albumin, 8 parts of Freund's complete adjuvant, 2 parts of Freund's incomplete adjuvant, HRP- 3 parts of goat anti-mouse IgG, 6 parts of ammonium sulfate, 10 parts of disodium hydrogen phosphate, 20 parts of potassium dihydrogen phosphate, 16 parts of citric acid, 12 parts of ammonium dihydrogen phosphate, 15 parts of absolute ethanol, 3 parts of isobutyl chloroformate 4 parts of coating buffer, 7 parts of blocking solution, 4 parts of washing buffer, 10 parts of substrate, and 25 parts of cell culture medium.

[0054] The coating buffer is selected from a carbonate buffer with a concentration of 0.05mol / L and a pH value of 9.6; the blocking solution uses 2g of imported gelatin, which is obtained by adding 100ml of coating buffer to ...

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Abstract

The invention discloses a doxycycline broad-spectrum monoclonal antibody and a preparation technology. The doxycycline broad-spectrum monoclonal antibody is prepared from the following raw materials in parts by weight: 40-52 parts of doxycycline, 4-20 parts of tetracycline, 2-6 parts of minocyclin, 2-4 parts of metacycline, 3-5 parts of bovine serum albumin, 5-8 parts of a Freund's complete adjuvant, 1-2 parts of Freund's incomplete adjuvant, 2-3 parts of HRP-Goat anti Mouse IgG, 3-6 parts of ammonium sulphate, 9-10 parts of disodium hydrogen phosphate, 16-20 parts of potassium dihydrogen phosphate, 12-16 parts of citric acid, 8-12 parts of ammonium dihydrogen phosphate, 9-15 parts of anhydrous ethanol, 1-3 parts of isobutyl chlorocarbonate, 2-4 parts of a coating buffered solution, 5-7 parts of confining liquid, 3-4 parts of a washing buffered solution, 6-10 parts of substrates and 20-25 parts of cell culture fluid, wherein the doxycycline and 4-Aminophenylacetic acid are used as main raw materials, and a diazo method is used for preparing the doxycycline broad-spectrum monoclonal antibody having antisera specificity. The doxycycline broad-spectrum monoclonal antibody has excellent biological properties, and is good in antisera titer and rejection capability.

Description

technical field [0001] The invention relates to the field of doxycycline antibody preparation technology, in particular to a doxycycline broad-spectrum monoclonal antibody and a preparation technology. Background technique [0002] Doxycycline is one of the important members of tetracycline antibiotics. It is widely used clinically in animal husbandry and veterinary. The accumulation of residues in feeding tissues not only leads to the emergence of drug-resistant bacteria, but also seriously affects the export of animal husbandry products in my country, causing major economic losses, and also brings great harm to the physical and mental health of consumers. In addition, breeders also There are medication regulations that do not comply with the dosage, administration site, and species of animals used, as well as the phenomenon of repeated use of several drugs with different trade names but the same ingredients. Therefore, it is necessary to strengthen the monitoring of veterina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K1/34
CPCC07K16/12
Inventor 王作弟
Owner 陕西思尔生物科技有限公司
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