Dry chemical bigeminal reagent strip for simultaneously detecting glutamic oxalacetic transaminase and glutamic-pyruvic transaminase

Abstract

The invention discloses a dry chemical bigeminal reagent strip for simultaneously detecting glutamic oxalacetic transaminase and glutamic-pyruvic transaminase. The dry chemical bigeminal reagent strip is characterized in that two sections of test paper pads are contained, and each test paper pad is composed of four layers of films which are the diffusion film on the surface layer, the rapid glass fiber filter film on the second layer, the mixed fiber film on the third layer and the reaction film on the bottom layer. Corresponding reactants are adsorbed on the reaction films, and when a detected object is added into a sample inlet hole, the detected object is uniformly dispersed on the two test paper pads through the surface diffusion films and then reaches the reaction films on the bottom layer to carry out reacting. Meanwhile, a lower supporting layer of the reagent strip is provided with two test holes which are connected with the reaction films on the bottom layer respectively, in the reacting process, dihydrocoenzyme I (NADH) adsorbed on the reaction films is oxidized into NAD+, the descend range of the absorbance at 340 nm is tested, and therefore the content of the corresponding tested object is obtained. The reagent strip can be used for rapidly determining the activity of glutamic oxalacetic transaminase and glutamic-pyruvic transaminase in the human whole blood or serum or plasma, and therefore clinical monitoring and adjuvant therapies of liver diseases are promoted.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection, in particular to a method for simultaneously detecting aspartate aminotransferase and alanine aminotransferase. Background technique [0002] Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) mainly exist in various cells, especially in liver cells. Normally, only a small amount exists in the blood. In the acute phase of various viral hepatitis, AST and ALT are released into the blood in large quantities when the liver cells are necrotic due to drug poisoning. Therefore, these two transaminases are important indicators for the diagnosis of viral hepatitis and toxic hepatitis. At the same time, it is also a very sensitive indicator for hepatitis patients. The higher the transaminase generally reflects the more serious the damage of liver cells, and to some extent, it indicates the severity and prognosis of the disease. The AST / ALT ratio has certain clinical g...

AI Technical Summary

Problems solved by technology

[0010] At present, most medical equipment detects the two indicators of AST and ALT separately, which is time-consuming and wastes resources. Reagent strips

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