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UPLC method for simultaneously determining eight components in unique medicinal material

A unique herbal medicine technology, applied in the UPLC field of simultaneous determination of 8 components in unique herbal medicines, can solve the problems of long detection time and low efficiency

Inactive Publication Date: 2017-01-11
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection time of HPLC method is long and the efficiency is not high

Method used

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  • UPLC method for simultaneously determining eight components in unique medicinal material
  • UPLC method for simultaneously determining eight components in unique medicinal material
  • UPLC method for simultaneously determining eight components in unique medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Method of the present invention and methodology investigation

[0043] 1 material

[0044] Prominence UFLC XR ultra-fast liquid chromatography (Shimadzu, Japan), BSA124S electronic balance (Sartorius, Germany), UPH-I-10T ultrapure water device. The reference substances of catalin, shanzhiside methyl ester, chlorogenic acid, caffeic acid, 8-O-acetyl shanzhiside methyl ester, forsythiaside B, luteolin, and ergosterin were all from Chengdu Pusi Biotechnology Co., Ltd. Co., Ltd., acetonitrile is chromatographically pure, water is ultrapure water, and other reagents are analytically pure. 32 batches of samples of Duyiwei were collected from different places of origin, as shown in Table 1, which were identified as the dry aerial part of Duyiwei Lamiophlomis rotata (Benth.) Kudo by Associate Professor Zhong Shihong of Chengdu Medical College.

[0045] Table 1 Sources of unique herbal samples

[0046]

[0047] 2 Methods and results

[0048] 2.1 Chromatograph...

Embodiment 2

[0073] The process screening of embodiment 2 the inventive method

[0074] (1) Detection wavelength screening

[0075] The full-wavelength scanning of the S2 extract showed that catarin, shanzhiside methyl ester, and 8-O-acetylshanzhiside methyl ester had the maximum absorption at 238nm, caffeic acid had the maximum absorption at 323nm, and chlorogenic acid had the maximum absorption at 326nm. The maximum absorption, luteolin has the maximum absorption at 345nm, and forsythiaside B and ergoside have the maximum absorption at 330nm, and the absorption spectra of 8 components are combined to finally determine the genus genus glucoside, shanzhiside methyl ester, 8-O - 238nm was selected as the detection wavelength for acetylshanzhiside methyl ester, 320nm was selected as the detection wavelength for caffeic acid, chlorogenic acid, forsythiaside B, and ergosteroside, and 350nm was selected as the detection wavelength for luteolin.

[0076] (2) Extraction method screening

[0077...

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Abstract

The invention discloses a UPLC method for simultaneously determining eight components in a unique medicinal material. The eight components are sesamoside, shanzhiside methyl ester, chlorogenic acid, caffeic acid, 8-O-acetyl shanzhiside methyl ester, forsythiaside B, galuteolin and acteoside. The method can effectively detect and separate the eight components, the content of the components can be accurately, reliably, easily, conveniently and rapidly determined, and effective guarantees are provided for comprehensively monitoring the quality of the unique medicinal material.

Description

technical field [0001] The invention relates to the technical field of medicine quality control methods, in particular to a UPLC method for simultaneously determining eight components in unique medicinal materials. Background technique [0002] Lamiophlomis rotata (Benth.) Kudo is a traditional Tibetan herbal medicine, a labiatae plant, mainly distributed in my country's Tibet, Yunnan, Sichuan, Qinghai, Gansu and other places. The medicinal part is the unique dry aboveground part, which grows on the plateau at an altitude of 2700-4500 meters or in the strongly weathered gravel beach on the mountain or in the stony alpine meadow and river beach (Flora of Sichuan, Flora of Yunnan), mainly The ingredients are flavonoids, iridoids and phenylethanol glycosides. [0003] At present, the method for determining various components in the unique medicinal material is usually the HPLC method. However, the detection time of the HPLC method is long and the efficiency is not high. At t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 钟世红苟燕梅古锐
Owner CHENGDU MEDICAL COLLEGE