A kind of method of ULC determination of flavonoids in Astragalus root extract
A technology of astragalus extract and flavonoids, applied in the field of determination of four flavonoids in astragalus extract, can solve the problem of lack of detection methods for flavonoids
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Embodiment 1
[0115] The chromatographic conditions and system suitability test instrument is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.2% formic acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, the gradient setting is shown in the table below, and the flow rate is 0.4mL / min; detection wavelength: 254nm.
[0116]
[0117] Preparation of the reference substance solution Take an appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add 70% methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and Formononetin is a solution of 0.01 mg, 0.04 mg, 0.004 mg and 0.15 mg, respectively.
[0118] Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 70% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, ...
Embodiment 2
[0124] Chromatographic conditions and system suitability test instrument is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.1% formic acid aqueous solution as mobile phase A, 0.1% formic acid acetonitrile as mobile phase B, the gradient settings are shown in Table 1, the flow rate 0.3mL / min; detection wavelength: 254nm.
[0125] Preparation of reference substance solution Take appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and formononetin Anthocyanins are 0.01mg, 0.04mg, 0.004mg and 0.15mg solutions respectively.
[0126] Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 50% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, and you get it.
...
Embodiment 3
[0129] Chromatographic conditions and system suitability test equipment is Waters ACQUITY ultra-high performance liquid chromatography, using UPLCHSS T3 chromatographic column, with 0.5% formic acid aqueous solution as mobile phase A, 0.5% formic acid acetonitrile as mobile phase B, the gradient settings are shown in Table 1, the flow rate 0.6mL / min; detection wavelength: 280nm.
[0130] Preparation of reference substance solution Take appropriate amount of calycosin, calycosin glucoside, formononetin and formononetin reference substance respectively, add methanol to prepare each milliliter containing calycosin, calycosin glucoside, formononetin and formononetin Anthocyanins are 0.01mg, 0.04mg, 0.004mg and 0.15mg solutions respectively.
[0131]Preparation of the test solution Take about 0.5 g of Radix Astragali extract, weigh it accurately, add an appropriate amount of 50% methanol solution, dissolve it with ultrasonic waves, dilute to 25 ml, shake well, and you get it.
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