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Template for DYS389 locus allelic discrimination standard

A typing standard and allele technology, applied in the field of forensic medicine, can solve the problems that the flatness of the allelic typing standard cannot be met and the peak heights of difficult-to-amplify fragments are equalized.

Active Publication Date: 2017-01-25
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To prepare allelic typing standards, it is necessary to screen blood card samples containing different allelic typing, but the lengths of the two amplified fragments on the DYS389 locus are different and there is an inclusion relationship between them, and the differences in different blood card samples make After amplification, two amplified fragments with different peak heights randomly appear, and it is difficult to level them according to the peak heights of the two amplified fragments, which cannot meet the requirements for the flatness of allelic typing standards

Method used

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  • Template for DYS389 locus allelic discrimination standard
  • Template for DYS389 locus allelic discrimination standard
  • Template for DYS389 locus allelic discrimination standard

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0066] Example 1. Preparation and verification of templates for allelic typing standards of DYS389 locus

[0067] 1. Obtaining recombinant plasmid

[0068] 1. Optimization of connection sequence

[0069] hg19 human genome (for information on the hg19 human genome, see the website

[0070] http: / / hgdownload.soe.ucsc.edu / goldenPath / hg19 / bigZips / hg19.2bit ), the DYS389 locus contains the DNA fragment shown in the linking sequence 1. The linking sequence 1:

[0071] 5’-TCATTATACCTACTTCTGTATCCAACTCTCATCTGTATTATCTATGTA-3’. After a lot of experiments, the connection sequence 1 is optimized to the connection sequence 2:

[0072] 5'-TCTCTTACTATCATGCCATCCTTAAGATTCTTTTCTGTAACTCATAAT-3'. After evaluation by the software OligoAnalyzer 3.1, the basic characteristics of connection sequence 1 and connection sequence 2 are shown in Table 1.

[0073] Table 1

[0074] Number of bases (bp) GC content (%) Melting temperature (℃) Connection sequence 14831.260.4 Connection sequence 24831.261.5

[0075] ...

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Abstract

The invention discloses a standard template for DYS389 locus allelic discrimination. The standard template for DYS389 locus allelic discrimination is a DNA composition, the DNA composition includes a DNA segment 1 - DNA segment 17, the nucleotide sequence is indicated as sequence 1, sequence 2, sequence 3, sequence 4, sequence 5, sequence 6, sequence 7, sequence 8, sequence 9, sequence 10, sequence 11, sequence 12, sequence 13, sequence 14, sequence 15, sequence 16, and sequence 17 in the sequence table. The experiment shows, the template provided by present invention can be augmented to obtain the DYS389 locus allelic discrimination standard, can be used in a variety of commercial kits, and has significant application value.

Description

Technical field [0001] The present invention relates to the technical field of forensic medicine, in particular to templates for allelic typing standards of the DYS389 locus. Background technique [0002] Humans have 22 pairs of autosomes and 1 pair of sex chromosomes. There are many polymorphic DNA genetic markers on the sex chromosomes. In some special cases (such as the crime of sexual assault and the identification of some genetic relationships), the analysis of genetic markers of sex chromosomes has Important value. The STR locus of the Y chromosome is one of the important genetic markers of Y-DNA. Since the Y chromosome is linked to inheritance and is haplotyped, it does not recombine with other chromosomes during subtractive classification except for the pseudo-euchromatic region. Therefore, a single sample only detects one allele in most Y-STR loci gene. However, there are a few Y-STR loci that can detect multiple alleles, such as DYS389 locus, DYS385 locus, DYS527 loc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6876C12Q2600/156C12Q2600/166C12Q2531/113C12Q2545/107
Inventor 王乐赵兴春季安全叶健武波
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY