Alcaligenes sp. and application thereof to high-salt high-polymer waste water

A technology of high-salt wastewater and alcaligenes, which is applied in the direction of bacteria, special compound water treatment, biological water/sewage treatment, etc., can solve the problem of unsatisfactory treatment effect, reduce the biochemical footprint, improve biochemical efficiency, The effect of improving the degradation efficiency

Active Publication Date: 2017-02-15
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many reports at home and abroad on the treatment of wastewater containing macromolecular organic substances such as polysulfonic substances and polyacrylamide, the effect of microbial treatment on refractory wastewater with a conductivity of 15000-38000us / cm and a COD of 3500-7000mg / L has been consistent. It is not very ideal, so find one or more strains that can survive in high-salt wastewater with a conductivity of 15000-38000us / cm, and can efficiently degrade sulfonic substances and polyacrylamide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation method of embodiment 1 microbial bacterial agent and the application in producing polyacrylamide plant waste water

[0015] The bacterial strain Z-1 of the present invention is obtained from the bottom mud of the drilling wastewater storage tank through acclimation culture, screening and isolation.

[0016] 1. Biological characteristics of Z-1

[0017] Z-1 is a monococcus, Gram-negative bacteria. On solid medium, the colonies are round, white, smooth, moist and shiny, with neat edges and non-sticky.

[0018] 2. To identify the 16S rDNA of strain Z-1, the specific steps are as follows:

[0019] Forward primer F1: 5'-AGAGTTTGATCCTGGCTCAG-3', reverse primer R1: 5'-GGTTACCTTGTTACGACTT-3', for DNA amplification (PCR). PCR reaction conditions: pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 58°C for 30 s, extension at 72°C for 90 s, 35 cycles, final extension at 72°C for 10 min, storage at 4°C. PCR products were separated by...

Embodiment 2

[0037] Example 2: Treatment of Drilling Wastewater from an Offshore Platform by Z-1 Bacteria

[0038]The difference from Example 1 is that the COD in the drilling wastewater of an offshore platform is 5000-7000mg / L, among which the COD provided by polyacrylamide accounts for about 5% of the total COD, and the COD provided by polysulfonic macromolecules accounts for about 6% of the total COD. %-10%, the conductivity of wastewater is 27000-35000us / cm. Use Z-1 bacteria to treat this wastewater, the dosage of the bacteria is 2‰, and after 48 hours of aerobic treatment, the COD of the effluent will reach below 1500mg / L; the growth promoter is added at the same time as the bacteria are added, and the growth promoter provides The COD is about 200mg / L, the effluent COD is below 500mg / L, and the content of polyacrylamide and polysulfonate is less than 0.2%.

example 3

[0039] Example 3: Treatment of a certain natural gas drilling waste water by Z-1 bacteria

[0040] The difference from Example 1 is that the treated wastewater is natural gas drilling wastewater in a certain place in Shanxi, the effluent COD is 5500mg / L, and the conductivity is 25000-28000us / cm, among which macromolecules such as polysulfonate account for about 8%, and 0.5‰Z is added -1 bacteria treatment, after 48 hours, the COD of the effluent reaches 500mg / L, and the proportion of macromolecules such as polysulfones is below 0.3%.

[0041] Principle of invention

[0042] Alcaligenes Z-1 is a bacterium that can degrade polyacrylamide, polysulfone and other macromolecular organic substances, which is isolated and screened from the bottom mud of drilling wastewater storage tanks. In the living environment of high-salt wastewater with a conductance of 15000-38000us / cm, Z-1 first produces biohydrolytic enzymes that have a certain degradation ability for polymers, first attacks ...

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Abstract

The invention discloses a strain of alcaligenes sp. and application thereof to high-salt high-polymer waste water. The collection number of the alcaligenes sp. Z-1 provided by the invention is CGMCC No.12585. The alcaligenes sp. can effectively decompose macromolecular polymers, such as polyacrylamide, polysulfonate substances and the like, into micromolecular substances easy to degrade under the environment of high-salt high-polymer waste water, and can obtain energy by decomposing the organisms to maintain growth and reproduction. In the high-salt high-polymer waste water which contains the organisms, such as polyacrylamide, polysulfonate substances and the like, and is treated by the bacteria, the decomposition rate of the high polymer can reach above 95%, the COD (chemical oxygen demand) of the waste water can be effectively reduced and the COD removal rate can reach above 50%.

Description

technical field [0001] The invention belongs to the field of microorganism and wastewater treatment, and relates to an alcaligenes Z-1 and its application in high-salt and high-polymer wastewater. Background technique [0002] my country's petroleum and chemical industry produces a large amount of high-salt and refractory organic wastewater every year. These organic wastewater often contain a large number of artificially synthesized macromolecular polymer additives. These polymers have a large molecular weight and complex structure, are difficult to degrade, and exist in the environment for a long time. It is more harmful to the environment and people. The direct discharge of such wastewater will cause serious pollution to surface water, groundwater, soil, plants and the ecological environment, causing many negative impacts on the survival and development of enterprises, and has become a matter of great concern to the society, the public and government departments. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/05
CPCC02F3/34C02F2305/06C12N1/205C12R2001/05
Inventor 杨勇霍莹张艳芳郑贝贝张莹付连超
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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