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Quality detection method of hippophae rhamnoides fruit oil

A quality detection method and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of uneven quality of market products, unidentifiable quality control methods, and inferior quality of seabuckthorn fruit oil, etc., to achieve simple steps, Anti-adulteration, good reproducible effect

Active Publication Date: 2017-02-15
SHAANXI BUCHANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the wide application of seabuckthorn fruit oil in the market and the high cost of production and processing, seabuckthorn fruit oil products in the market are mixed with other low-cost vegetable oils, and the existing quality control methods cannot be identified, resulting in uneven product quality in the market
Modern medicine believes that palmitic acid and palmitoleic acid are effective components in seabuckthorn fruit oil, but effective quality control of the two components in seabuckthorn fruit oil has not yet been carried out. Gas chromatography is used to determine the content of the two components. The content limit and proportion relationship of the three components are of great significance for the quality control of seabuckthorn fruit oil to identify the inferior quality of seabuckthorn fruit oil

Method used

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  • Quality detection method of hippophae rhamnoides fruit oil
  • Quality detection method of hippophae rhamnoides fruit oil
  • Quality detection method of hippophae rhamnoides fruit oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] (1) Preparation of reference substance solution: Accurately weigh 50 mg each of palmitic acid and palmitoleic acid reference substance, put them in a 20mL test tube, add 2mL of methanol, shake well, add 0.5moL·L -1 Sodium hydroxide methanol solution 2mL, after filling with nitrogen, heat in a water bath at 50°C for 5min, add 2.0mL of boron trifluoride ether solution, after filling with nitrogen, continue heating in a water bath at 50°C for 10min, transfer to a separatory funnel, and rinse the test tube with water 3 times, 10 mL each time, put the washing liquid into a separatory funnel, extract 3 times with ether, 20 mL each time, combine the ether solution, filter the ether solution with anhydrous sodium sulfate, combine the ether solution, and dry the ether solution with nitrogen flow , add n-hexane to move the residue to a 50mL volumetric bottle, dilute to the mark, shake well, and use it as the reference substance stock solution, accurately measure 5mL of the referen...

Embodiment 2

[0075] (1) Preparation of reference substance solution: Accurately weigh 50 mg each of palmitic acid and palmitoleic acid reference substance, put them in a 20mL test tube, add methanol 2mL, add 0.5moL·L -1 Sodium hydroxide methanol solution, after filling with nitrogen, heat in a water bath at 50°C for 3 minutes, add 1 mL of boron trifluoride ether solution, after filling with nitrogen, continue heating in a water bath for 13 minutes, transfer to a separatory funnel, rinse the test tube with water 4 times, wash The solution was combined into a separatory funnel, extracted with diethyl ether 4 times, 15 mL each time, the diethyl ether solution was combined, the diethyl ether solution was filtered with anhydrous sodium sulfate, the diethyl ether solution was combined, the ether solution was blown dry with a nitrogen stream, and the residue was removed by adding n-hexane Put it into a 50mL measuring bottle, dilute to the mark, shake well, and use it as the reference substance sto...

Embodiment 3

[0081] (1) Preparation of reference substance solution: Accurately weigh 50 mg each of palmitic acid and palmitoleic acid reference substance, put them in a 20mL test tube, add methanol 2mL, add 0.5moL·L -1 Sodium hydroxide methanol solution, after filling with nitrogen, heat in a water bath at 50°C for 7 minutes, add 3 mL of boron trifluoride ether solution, after filling with nitrogen, continue heating in a water bath for 7 minutes, transfer to a separatory funnel, rinse the test tube twice with water, wash The solution was combined into a separatory funnel, extracted twice with diethyl ether, 25 mL each time, the diethyl ether solution was combined, the diethyl ether solution was filtered with anhydrous sodium sulfate, the diethyl ether solution was combined, the ether solution was blown dry with a nitrogen stream, and the residue was removed by adding n-hexane Put it into a 50mL measuring bottle, dilute to the mark, shake well, and use it as the reference substance stock so...

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Abstract

The invention discloses a quality detection method of hippophae rhamnoides fruit oil. The detection method comprises the following steps: preparation of a control solution; preparation of a test solution, and determination of chromatographic conditions and content, wherein the chromatographic conditions are as follows: a capillary chromatographic column and an FID detector are included, the temperature of a sample inlet is 190-210 DEG C, and the temperature of a detector is 200-230 DEG C; temperature programming is performed, the initial temperature is 50-80 DEG C, is maintained for 1-5 min, is increased to 160-190 DEG C at a rate of 20-50 DEG C.min<-1> and is then increased to 190-210 DEG C at a rate of 1-2.5 DEG C.min<-1>, and the increased temperature is maintained for 10-15 min; the split ratio is (30-60):1. The method is high in accuracy and good in repeatability, and the quality of the hippophae rhamnoides L fruit oil can be identified and evaluated according to the content and proportional relation between determined components of palmitic acid and palmitoleic acid.

Description

technical field [0001] The invention relates to the field of detection methods of Chinese patent medicines, in particular to a quality detection method of seabuckthorn fruit oil. Background technique [0002] Seabuckthorn (Hippophae rhamnoides L.) is a deciduous shrub or small tree of the genus Acanthopanax in the family Echinacea. It is drought-tolerant, drought-tolerant, salt-alkali-resistant, and has strong adaptability. Distributed in Inner Mongolia, Gansu, Shanxi and other places. Seabuckthorn fruit oil is the effective part isolated and extracted from fresh seabuckthorn fruit. It mainly contains fatty acids and fat-soluble vitamins. It has pharmacological effects such as promoting fat metabolism in the body and reducing blood cholesterol levels. There are many health products and medicines with seabuckthorn fruit oil as the main raw material on the market. Due to the wide application of seabuckthorn fruit oil in the market and the high cost of production and processi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 刘峰李炜陈衍斌马久太王浩仁李瑾陈英许刚苏英英张利孙建敏卢新义惠大永吴云生
Owner SHAANXI BUCHANG PHARMA
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