A standard gene sequence of kudzu dna barcode and its application

A barcode and gene technology, applied in the direction of recombinant DNA technology, DNA / RNA fragments, biochemical equipment and methods, etc., can solve the problems of unsuitable industrialization and popularization of detection, high requirements for samples to be measured, and complex result judgment methods, etc. problems, to achieve the effects of shortening the identification time, simple result determination, and low cost
CN108220472BActive Publication Date: 2021-02-23SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
Publication Date
2021-02-23

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Abstract

The present invention relates to a standard gene sequence of a kudzu DNA barcode and its application. The standard detection gene sequence of the barcode is shown in SEQ ID NO.1. The genome of Pueraria mirifica was amplified by polymerase chain reaction. According to the size judgment result of the amplified product of the tested sample, if a band of 226bp can be specifically amplified, it can be judged that the tested sample contains kudzu components. . The invention provides a method for specifically detecting kudzu components, which is helpful for the identification of kudzu components, prevents adulteration and ensures the health of consumers. Compared with the traditional morphological identification method, the gene sequence obtained by the invention is beneficial to realize the molecular identification of kudzu, and can effectively shorten the identification time of kudzu.
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Description

technical field

[0001] The invention relates to the field of species identification, in particular to a kudzu species-specific gene sequence and a primer composition thereof. Background technique

[0002] Pueraria lobata (scientific name: Pueraria lobata, English name: kudzu) is a perennial deciduous vine of the genus Pueraria. Except for Xinjiang, Qinghai and Tibet, it is distributed several times throughout the country. Pueraria mirifica is a sun plant, and it often grows in patches on the edge of the forest on the sunny slope of 100-2000 meters above the poster or in the bushes by the river. The whole body of Pueraria mirifica is treasure, and its root, stem, leaf, flower, fruit (seed) etc. all have higher edible value, medicinal value and economic value. Pueraria lobata "Compendium of Materia Medica" records: Pueraria lobata has a sweet, flat, non-toxic smell. It has the effects of clearing heat and detoxifying, relieving spasm and relieving pain, promoting yang and r...

Claims

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