Application of a Multiple Linear Regression Model in Predicting Abdominal Fat Mass of Broilers or Breeding Low-fat Broilers
A multiple linear regression and abdominal fat technology, applied in the field of poultry genetics and breeding, can solve the problems of insignificant correlation, etc., and achieve the effects of reducing the difficulty of blood collection, reducing seed selection errors, and preventing hemolysis
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Embodiment 1
[0022] 1. Experimental materials
[0023] 1. Experimental Animals and Determination of Traits
[0024] The 18th generation roosters of broiler high-fat and low-fat two-way selection line selected by Northeast Agricultural University were used as the experimental group. At the age of 46 days and 48 days, after the broilers had eaten, blood was collected from the subwing vein, and the blood sample was placed in a 1.5ml centrifuge tube (EDTA-Na2 was used as an anticoagulant) and centrifuged at 3000r / min for 15min to obtain plasma. Put it into an EP tube and store it in a -20°C refrigerator for later use. At the age of 7 weeks, the live weight was measured before slaughtering, and the abdominal fat weight was measured after slaughtering, and the percentage of abdominal fat was calculated by dividing by the live weight at 7 weeks old.
[0025] 2. Drugs and enzymes
[0026] Triglyceride detection kit; total cholesterol detection kit; high-density lipoprotein cholesterol detection...
Embodiment 2
[0043] Example 2 Comparison of Chicken Plasma Biochemical Indexes Between High and Low Fat Two-way Selection Line Broilers
[0044] The 18th generation broiler chickens of high-fat and low-fat two-way selection line selected by Northeast Agricultural University were used as experimental materials to compare and analyze the abdominal fat traits of high-fat and low-fat broiler chickens. The results showed that the abdominal fat weight and abdominal fat rate of high-fat broilers were significantly higher than those of low-fat broilers (Table 1, P<0.01).
[0045] Table 1 Comparison of abdominal fat traits between high and low-fat broilers
[0046]
[0047] Note: ** P<0.01.
[0048]The 18th generation broiler chickens of high-fat and low-fat two-way selection line selected by Northeast Agricultural University were used as experimental materials to compare and analyze the plasma biochemical indicators between high-fat and low-fat broiler chickens. The results showed that the p...
Embodiment 3
[0053] Example 3 Phenotypic correlation between plasma biochemical indicators and abdominal fat traits
[0054] Phenotypic correlation analysis was carried out between the plasma biochemical indexes and abdominal fat traits in Example 2, and the results showed that plasma triglycerides, total bile acids, total proteins, globulins, γ-glutamyl transpeptidase, uric acid, creatinine and The level of VLDL had a very significant positive phenotype correlation with abdominal fat traits (P0.05).
[0055] Table 3 Phenotypic correlation between plasma biochemical indicators and abdominal fat traits
[0056]
[0057]
[0058] Note: * P** P<0.01; HDL-C high-density lipoprotein cholesterol; LDL-C low-density lipoprotein cholesterol; VLDL very low-density lipoprotein.
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