Application of sorafenib combined with CCR4 antagonist for restraining cancer growth and transfer

An antagonist and antagonism technology, applied in the field of biomedicine, can solve the problems of tumor growth and metastasis that have not yet been seen

Active Publication Date: 2017-02-22
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there have been no reports on the combination of sorafenib and CCR4 antagonists for the treatment of liver cancer, kidney cancer, non-small cell lung cancer, melanoma, thyroid cancer, breast cancer, prostate cancer and other tumor growth and metastasis

Method used

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  • Application of sorafenib combined with CCR4 antagonist for restraining cancer growth and transfer
  • Application of sorafenib combined with CCR4 antagonist for restraining cancer growth and transfer
  • Application of sorafenib combined with CCR4 antagonist for restraining cancer growth and transfer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Compound

[0032] 1. Sorafenib API was purchased from Yaodu Company.

[0033] 2. The small molecular compound c-021 was purchased from Tocris Company (product number: 3581, purity > 99%), and the molecular formula of the compound is C 27 h 41 N 5 o 2 2HCl, the structure is as follows:

[0034]

[0035] 3. The small molecular compound ST45177901 was purchased from Timtec Company (article number: MFCD04147999, purity > 99%), and the molecular formula of the compound is C 26 h 16 ClN 3 o 4 S 3 , with the following structure:

[0036]

Embodiment 2

[0037] Example 2: Detection of changes in chemokines and cytokines in liver cancer cell lines treated with Sorafenib

[0038] Real-time fluorescence quantitative PCR was used to detect the changes in the secretion levels of chemokines and cytokines after stimulation of liver cancer cell lines with different concentrations of sorafenib, and to screen out the highly secreted factors induced by sorafenib.

[0039] 1. Cells

[0040] Mouse hepatoma cell line Hepa1-6 cells were purchased from ATCC Cell Bank (Product No.: CRL-1830).

[0041] 2. Drugs

[0042] Treat Hepa1-6 cells with different concentrations of sorafenib for 48h:

[0043] Experimental group 1: 4 μM sorafenib was added to the cell culture medium and cultured for 48 hours;

[0044] Experimental group 2: 2 μM sorafenib was added to the cell culture medium and cultured for 48 hours;

[0045] Control group: add an equal volume of DMSO and culture for 48 hours.

[0046] 3. Experimental equipment

[0047] Instrument n...

Embodiment 3

[0051] Example 3: Animal experiment of Sorafenib combined with CCR4 antagonist for the treatment of liver cancer growth and metastasis

[0052] The mouse subcutaneous tumor-bearing model is a model for routine observation of tumor growth and metastasis, so it is used to verify the effect of Sorafenib combined with CCR4 antagonists on the growth and metastasis of liver cancer.

[0053] 1. Animals

[0054] C57BL / 6 mice (all male, weighing 20-24 g, 6 mice per group / cage) were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and were raised in an SPF animal room. A total of 6 groups were divided into experiments.

[0055] 2. Cells

[0056] The mouse liver cancer cell line Hepa1-6 cells were purchased from the ATCC cell bank (product number: CRL-1830), and each mouse was inoculated with 3*10 6 cells, and experimental mice were obtained.

[0057] 3. Drugs

[0058] Group the experimental mice into groups:

[0059] Experimental group 1: intragastric a...

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Abstract

The invention relates to an application of sorafenib combined with a CCR4 antagonist for restraining cancer growth and transfer, belongs to the technical field of biological medicine and specifically relates to the application of a sorafenib drug and a CCR4 antagonist, especially, combination of C-021 and ST45177901, for restraining the growth and transfer of tumors, such as, liver cancer, kidney cancer, lung cancer, breast cancer, melanin cancer, thyroid cancer and prostate cancer. The compound combined treatment provided by the invention has excellent clinical application prospects in restraining the growth and transfer of the tumors, especially liver cancer, kidney cancer, lung cancer, breast cancer, melanin cancer, thyroid cancer and prostate cancer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of sorafenib combined with CCR4 antagonists in inhibiting cancer growth and metastasis. Background technique [0002] Sorafenib (sorafenib) is an oral multikinase inhibitor, which can effectively inhibit tumor cell proliferation and angiogenesis, and has a significant therapeutic effect on liver cancer and kidney cancer. It has also been reported in the treatment research of breast cancer, prostate cancer and other cancers. [0003] Liver cancer is one of the most common clinical malignant tumors, with the characteristics of high malignancy, rapid progression, and poor prognosis, which seriously threatens human health and life safety. China is the country with the highest incidence and the highest number of deaths from liver cancer in the world, accounting for about 55% of the world's liver cancer patients. In recent years, the incidence of liver cancer in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K31/517A61K31/433A61K31/44A61P35/00
Inventor 杨鹏远高雅楠杜成志
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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