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A fluid sample processing device for biochemical reactions

A biochemical reaction and sample processing technology, applied in the field of fluid processing devices, can solve problems such as complex fluid devices and processing procedures

Active Publication Date: 2019-04-26
XIAN TIANLONG SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, automating these processes requires complex fluidic devices and processing procedures, so there is still a need to develop such devices

Method used

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  • A fluid sample processing device for biochemical reactions
  • A fluid sample processing device for biochemical reactions
  • A fluid sample processing device for biochemical reactions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Such as figure 1 The fluid processing device shown is used for processing fluid samples, performing nucleic acid sample processing, extraction and purification, amplification, and fluorescent PCR detection, and can complete qualitative detection of multiple nucleic acid targets in the sample.

[0072] The device of this embodiment includes a sample injection port 1 for adding fluid samples and a sample processing chamber 3 for fluid sample lysis and nucleic acid adsorption. The sample injection port 1 and the sample processing chamber 3 are in communication with the flow channel 2; the flow channel 2 The outlet of the fluid is higher than the liquid level of the sample processing chamber 3 in the direction of fluid gravity, and the fluid sample is added to the sample inlet 1 and then reaches the sample processing chamber 3 through the flow channel 2.

[0073] The sample processing chamber 3 is used to complete fluid sample lysis and nucleic acid adsorption. The first reagent ...

Embodiment 2

[0085] The difference between this embodiment and embodiment 1, such as figure 2 As shown, the angle θ between the main flow channel 22 and each side flow channel 25 is equal to 90 degrees, the inlet of the flow channel 22 is in communication with the second reaction chamber 16, and the outlet of the flow channel 22 is the inlet of the side flow channel 25. The outlet is in communication with the liquid separation chamber 21. In this embodiment, the second reaction chamber 16 is connected and pressurized so that the eluent containing nucleic acid enters the liquid separation chamber 21 through the flow channel 22 and the side flow channel 25 for liquid separation.

Embodiment 3

[0087] This embodiment is different from embodiment 1 in that, as image 3 As shown, the liquid separation cavities 21 in the liquid separation detection cavity array are distributed on the main flow channel 22. In this embodiment, the liquid separation chamber array is connected to the second reaction chamber 16 through the main flow channel 22, and the second reaction chamber 16 is pressurized to make the nucleic acid eluate enter the liquid separation chamber array through the main flow channel 22 for liquid separation.

[0088] Each detection cavity 23 is pre-packaged with PCR solid reagents for nucleic acid amplification and fluorescence detection, which are used to realize the functions of PCR reaction system establishment, nucleic acid amplification, fluorescence detection and the like under the cooperation of external thermal cycling and optical detection devices. Each detection cavity 23 communicates with the liquid separation cavity array 21 through the flow channel arra...

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Abstract

The invention relates to a fluid sample processing device for a biochemical reaction. The device comprises a sample adding port, a sample processing chamber, at least two reaction chambers, a waste liquid chamber and a liquid separation detection chamber array; a fluid sample successively flows through the sample adding port, a sample processing chamber and at least two reaction chambers and then flows into the liquid separation detection chamber array or flows into the liquid separation detection chamber array and the waste liquid chamber by pressure and gravity; the sample processing chamber communicates with at least one reagent chamber, and each reaction chamber communicates with at least one reagent chamber. The device drives the liquid to flow by pressure and gravity, and relatively easily allows the fluid to smoothly enter and pass through the reaction chambers and the detection chambers. At the same time, the device can store a reagent in a form of vacuolar pre-encapsulation, can prevent the device from being additionally provided with a reagent storage structure, and reduces the total size of the device.

Description

Technical field [0001] The invention relates to a fluid processing device for biochemical reactions, in particular to a device and method for processing, separating, extracting, distributing and biochemical reactions of fluid biological, clinical or environmental samples. Background technique [0002] For example, the identification and analysis of fluid samples in laboratory testing, clinical testing or environmental monitoring generally includes a series of biochemical reaction processing steps, which may include chemical, optical, electronic, mechanical, thermal or acoustic processing of fluid samples . Whether it includes batch processing workstations, desktop instruments, or disposable kit-based POCT equipment, such processing processes typically include complex fluidic devices and processing procedures. [0003] People may have different purposes for analyzing fluid samples (such as biological samples, clinical samples, or environmental samples). One aspect is to develop a m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12M1/00
CPCC12Q1/686C12Q2563/107C12Q2565/629
Inventor 苗保刚李政彭年才李明田春孙瑶
Owner XIAN TIANLONG SCI & TECH
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