Microcystis aeruginosa culture method

A technology of Microcystis aeruginosa and its cultivation method, which is applied in the field of cultivation of Microcystis aeruginosa, can solve the problems such as the difficulty of pure cultivation of Microcystis aeruginosa, and achieve the effects of less pollution, fast growth rate and high survival rate

Inactive Publication Date: 2017-02-22
NANJING UNIV ECOLOGICAL RES INST OF CHANGSHU
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Problems solved by technology

However, as the main algae of water bloom outbreak, the laboratory pure cultivation of Microcystis is difficult, especially the existing cultivation methods are difficult to meet the requirements of laboratory purification and cultivation of Microcystis aeruginosa

Method used

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  • Microcystis aeruginosa culture method

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Experimental program
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Effect test

Embodiment 1

[0027] Preparation of algal seeds: Algal seeds were purchased from Microcystis aeruginosa FACHB-1343 from the Institute of Hydrobiology, Chinese Academy of Sciences.

[0028] Carry out indoor purification cultivation according to the above cultivation method, the incubator used for cultivation is Saifu constant temperature light incubator, and the density of inoculated algae is 5×10 4 cells mL -1 . Under sterile conditions, the algae liquid was taken every day, observed and counted under a Zeiss AxioScope A1 microscope with a hemocytometer, the algal cell density was calculated, and the growth curve was drawn. The results are shown in Figure 1.

[0029] It can be seen from Figure 1 that 0-4d is the lag phase of Microcystis aeruginosa growth, 4-12d is the logarithmic growth phase, and enters the stable phase after 12d. The number of algae cells in the stable period can reach 2300×10 4 cells mL -1 , and the algae liquid will not turn yellow during the cultivation process.

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Abstract

The invention relates to a microcystis aeruginosa culture method. The microcystis aeruginosa culture method includes the steps of a, previous preparation, b, microcystis aeruginosa preculture, and c, inoculation and culture of microcystis aeruginosa. The microcystis aeruginosa culture method has the advantages that the microcystis aeruginosa culture method is high in microcystis aeruginosa survival rate and microcystis aeruginosa growing rate, and a microcystis aeruginosa solution cannot yellow during culture and is less prone to being contaminated by other bacteria.

Description

technical field [0001] The invention relates to the field of agriculture, in particular to a method for cultivating Microcystis aeruginosa. Background technique [0002] At present, cyanobacteria blooms occur frequently in the world, among which Microcystis blooms are the most widely distributed and the most harmful. Among them, Microcystis aeruginosa is the most important algae that forms water blooms, and it often produces microcystins, which seriously threaten other aquatic organisms and even human health, so Microcystis aeruginosa is also the most harmful algae among cyanobacteria. In order to effectively prevent and control cyanobacterial blooms, it is particularly important to have a deep understanding of Microcystis aeruginosa. [0003] In order to explore effective ways to suppress algal blooms and better understand the natural conditions of algal blooms, a large number of laboratory simulations are required. However, as the main algae of water bloom outbreak, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 安树青张霞张红星
Owner NANJING UNIV ECOLOGICAL RES INST OF CHANGSHU
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