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Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device

A two-dimensional electrophoresis and oil dropper technology, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of uneven time interval, time-consuming and laborious, uneven oil dripping, etc., and achieve the effect of simple structure and easy operation

Active Publication Date: 2017-02-22
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the oil is dripped manually, which is not only time-consuming and laborious, but also the dripping time of the oil is uneven, including the position of the drop and the time interval between each drop, which affects the hydration effect of the strip and the subsequent protein separation. will have a certain impact

Method used

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  • Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device
  • Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device
  • Dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: as figure 1 As shown: 1 is a cylindrical barrel made of transparent material, and the lower end of the barrel 1 is connected with a liquid outlet 3 through a conical structure. The cylinder 1 is equipped with a piston 4 that can move axially along the cylinder 1 . The piston 4 is fixedly connected with one end of the piston rod 6 . The other end of the piston rod 6 extends to the outside of the barrel 1 . A communicating air hole 5 is provided at the axial center position or off-center position of the piston 4 and the piston rod 6 .

[0018] A handwheel 9 is connected to the end of the piston rod 6, and the piston rod 6 and the piston 4 can be rotated through the handwheel 9. The end of the piston rod 6 is provided with parts for closing and opening the air hole 5 . The parts for closing and opening the air hole 5 include a hose 10 set on the end of the piston rod 6, and an adjustment clamp 11 is provided on the hose 10. The adjustment clamp 11 can be ...

Embodiment 2

[0022] Such as figure 2 , 3 , 4: the difference from the embodiment is that the piston 4 axially moving position positioning member includes a raised block 15 connected to the piston rod 6 and located above the piston 4, and the inner wall of the cylinder body 1 is longitudinally provided with a plurality of intervals The baffle 14 and the thickness of the protruding block 15 match the distance between adjacent baffles 14 .

[0023] The parts for closing and opening the air hole 5 include a rotating circular plate 13, which is supported in parallel above the handwheel 9 through the rotating shaft 12. The rotating circular plate 13 and the handwheel 9 have an eccentric structure, and the rotating circular plate 13 just covers the piston rod 6. At the upper end face of the rotating circular plate 13, a circular hole 16 is provided, and the vertical distances between the circular hole 16, the air hole 5 and the rotating shaft 12 are equal.

[0024] During use, turn the rotatin...

Embodiment 3

[0026] Such as Figure 5 , 6 As shown, the difference from Embodiment 2 is that the parts for closing and opening the air hole 5 include a sunken arc-shaped groove 18 on the handwheel 9, a slider 17 is arranged in the arc-shaped groove 18, and a slider 17 is arranged on the slider 17. When the through hole 7 and the slider 17 slide in the arc-shaped groove 18, the through hole 7 can overlap or be staggered with the upper port of the air hole 5. The overlapping parts can adjust the speed of oil dripping. Others are related to the implementation Example 2 is the same and will not be described in detail.

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PUM

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Abstract

The invention discloses a dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device. The dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device comprises a transparent cylinder. A liquid outlet is formed in the lower end of the cylinder, a piston capable of axially moving along the cylinder is arranged in the cylinder, the piston is fixedly connected with one end of a piston rod, and the other end of the piston rod extends to the outer side of the cylinder; a communicated air hole is formed in the axial center position of the piston and the piston rod; a hand wheel is connected to the end of the piston rod, and a part for closing and opening the air hole is arranged on the end head of the piston rod; scales are arranged at the portion, located at the piston axial moving area, of the outer side of the cylinder; a piston axial-moving-position positioning piece is arranged in the cylinder. When the dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device is used, an upper end opening of the air hole is closed, oil liquid is absorbed into the cylinder by the liquid outlet through moving-up movement of the piston, then an opening closing part of the air hole is opened, the oil liquid can drop from the liquid outlet, the dropping speed of the time interval of the oil liquid can be adjusted through the opened size of the air hole, the oil liquid automatically and evenly drops, artificial control is not required, and time and labor are saved. The dimensional-electrophoresis adhesive-tape-hydration-sealing-layer quantitative oil dropping device is simple in structure and easy to control.

Description

technical field [0001] The invention relates to an oil dropper, in particular to a quantitative oil dropper for two-way electrophoresis strip hydration seal, which is mainly used for quantitative oil drop when two-way electrophoresis separates and analyzes proteins to form a strip hydration seal. layer. Background technique [0002] Two-dimensional polyacrylamide gel electrophoresis technology combines isoelectric focusing technology (separation according to protein isoelectric point) and SDS-polyacrylamide gel electrophoresis technology (separation according to protein size). Two-dimensional electrophoresis is the most effective electrophoresis method for separating and analyzing proteins. During the above-mentioned two-dimensional electrophoresis process, the hydration sealing layer of the rubber strip needs to be sealed by dripping oil. At present, most of the oil is dripped manually, which is not only time-consuming and laborious, but also the dripping time of the oil ...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/44704
Inventor 孙媛苗健郑旭李爱青张朋王晓炜张开立王茜刘晓宇马景昕刘铭吴茉莉程晓馨刘佳李宏
Owner DALIAN MEDICAL UNIVERSITY