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A γ-aminobutyric acid-producing Lactobacillus pentosus

A technology of Lactobacillus pentosus and aminobutyric acid, applied in the field of microorganisms, can solve the problems of low enrichment content, food safety, poor safety, high cost, etc., and achieve the effect of high production capacity of γ-aminobutyric acid

Active Publication Date: 2019-08-16
武汉蜜欢健康产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the early days, enrichment production of GABA was mainly based on chemical synthesis, plant enrichment and fermentation by Escherichia coli, but there were problems such as poor safety, high cost, serious environmental pollution, low enrichment content and food safety. , so it is of potential significance and value to search for available food-grade safe strains in the enrichment of GABA by microbial methods

Method used

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  • A γ-aminobutyric acid-producing Lactobacillus pentosus
  • A γ-aminobutyric acid-producing Lactobacillus pentosus
  • A γ-aminobutyric acid-producing Lactobacillus pentosus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation, screening and identification of bacterial strains

[0022] Isolation of strains: Select mature mulberry fruits from healthy growing mulberry trees, rinse them with sterile water for 3 times in a sterile ultra-clean bench, then dry them with sterile filter paper, cut them into small pieces of 1 cm, put them in a mortar and grind them to get the juice, and absorb 100 μL The fruit juice was cultured in the MRS liquid medium for 2 days in a constant temperature incubator at 30°C. When the liquid medium became turbid, the bacterial liquid was diluted by the gradient dilution method, and the dilution was 10. -5 and 10 -6 Put 100 μL of the bacterial cell dilution solution in the lactic acid bacteria isolation medium, and incubate in a 30°C constant temperature incubator for 2 to 3 days. After colonies grow in the lactic acid bacteria isolation medium (round milky white or yellow colonies appear), the surrounding medium turns yellow. It was determined to b...

Embodiment 2

[0032] The cultivation of embodiment 2 bacterial strains

[0033]1. Activation of Lactobacillus pentosus SS6: Aseptically operate in an ultra-clean workbench, pick Lactobacillus pentosus SS6 with an inoculation loop, streak on a plate medium (MRS medium) with an inoculation loop, and culture at 30°C Cultured in the box for 2 days.

[0034] 2. Preparation of fermentation samples of Lactobacillus pentosus SS6: Inoculate the isolated Lactobacillus pentosus SS6 into 50 mL of fermentation medium TYG, culture it in a 30°C incubator for 2 days, place the fermentation mixture in a boiling water bath for 10 minutes, and heat at 10000 r / h Centrifuge for 10 min, and take the upper liquid to obtain the fermentation broth.

[0035] 3. Drawing of γ-aminobutyric acid standard curve: Take 1.0mL distilled water and standard sample solutions of γ-aminobutyric acid with concentrations of 1mg / mL, 2mg / mL, 3mg / mL, 4mg / mL and 5mg / mL respectively, Add 120μL 1mol / L LaCl 3 Solution, mix and shake fo...

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Abstract

The present invention provides a γ-aminobutyric acid-producing Lactobacillus pentosus SS6. The Lactobacillus pentosus SS6 is preserved in the Guangdong Provincial Microbial Strain Preservation Center, and the preservation address is Guangdong Institute of Microbiology (Middle Xianlie Middle Road, Guangzhou City) 5th Floor, Building 59, No. 100 Courtyard), the preservation date is October 13, 2016, and the preservation number is GDMCC No: 60086; the yield of γ-aminobutyric acid produced by the fermentation of Lactobacillus pentosus SS6 reaches 3.09 mg / mL, and has The higher ability to produce GABA provides a basis for the development of new foods rich in GABA.

Description

technical field [0001] The invention relates to the technical field of microorganisms, more specifically, to a γ-aminobutyric acid-producing Lactobacillus pentosus. Background technique [0002] Gamma-aminobutyric acid (GABA) has many important physiological functions, such as anti-anxiety, lowering blood pressure, lowering blood sugar, improving sleep, repairing skin (inhibiting inflammation, stimulating epidermal cell regeneration), regulating hormones, etc. At this stage, with the continuous research and development of GABA's physiological and pharmacological functions, it has been used as a new type of functional factor in food and pharmaceutical industries. [0003] In the early days, enrichment production of GABA was mainly based on chemical synthesis, plant enrichment and fermentation by Escherichia coli, but there were problems such as poor safety, high cost, serious environmental pollution, low enrichment content and food safety. , so finding available food-grade s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/225
CPCC12N1/20C12P13/005C12N1/205C12R2001/225
Inventor 陈芳艳吴珊林健荣钟杨生
Owner 武汉蜜欢健康产业发展有限公司
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