Green method for increasing cellulose enzymolysis efficiency in rice straws by utilizing lactic acid/guanidine hydrochloride to extract hemicellulose
A technology of rice straw and cellulase, which is applied to fermentation and other directions, can solve the problems of unfriendly environment and high cost, and achieve the effects of overcoming unfriendly environment, low cost, and reducing equipment requirements and operation difficulty.
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[0021] Pretreatment of lactic acid / guanidine hydrochloride (molar ratio 1:1) improves the enzymatic hydrolysis efficiency of rice straw
[0022] a) Pretreatment: Accurately weigh 300mg of rice straw powder (the rice straw is dried and crushed into rice straw powder with an average particle size of 150-350μm) and 6g of lactic acid / guanidine hydrochloride deep eutectic solvent with a molar ratio of 1:1 , placed together in a 25mL Erlenmeyer flask, refluxed and stirred at 120°C for 6 hours; then cooled to room temperature, diluted with 6g of warm water, filtered, and washed with 18g of deionized water for 4 times, and the filter residue was pretreated after drying After the rice straw.
[0023] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer solution (50 mmol / L, pH 4.8) and 160 U of the commercial product derived from Trichoderma reesei Cellulase was sealed and placed in a constan...
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