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Amphoteric carboxylic acid three-dimensional metal coordination polymer, and preparation method and application thereof

A technology of coordination polymer and amphoteric carboxylic acid, applied in instruments, analytical materials, measuring devices, etc., can solve the problems of collapse of pore structure, poor water solubility, hindering the practical application research of nucleic acid molecule detection and so on.

Inactive Publication Date: 2017-03-15
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although metal coordination polymers with a three-dimensional pore structure have many advantages, some metal coordination polymers constructed with rigid ligands have poor water solubility and are prone to collapse of the pore structure when exposed to water, which seriously hinders the practical application of its nucleic acid molecular detection function. Research

Method used

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  • Amphoteric carboxylic acid three-dimensional metal coordination polymer, and preparation method and application thereof
  • Amphoteric carboxylic acid three-dimensional metal coordination polymer, and preparation method and application thereof
  • Amphoteric carboxylic acid three-dimensional metal coordination polymer, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 prepares metal coordination polymer

[0059] 1. Preparation of amphoteric carboxylic acid three-dimensional copper metal coordination polymer

[0060] (1) Disperse N-(3,5-dicarboxybenzyl)-(3-carboxy)-pyridine bromide (0.764g, 2mmol) in distilled water (70mL), adjust the pH to 7.0 with 0.1M NaOH solution, Obtain the main ligand solution;

[0061] (2) CuSO 4 ·5H 2 O (0.391g, 2mmol) was dissolved in distilled water (20mL) to obtain a copper salt solution;

[0062] (3) 1,2-bis(4-pyridyl)ethylene (0.360g, 2mmol) was dissolved in DMF solution (5mL) to obtain an auxiliary ligand solution;

[0063] (4) adding the copper salt solution obtained in step 2 dropwise to the main ligand solution obtained in step 1, stirring and reacting at room temperature for 0.5h and then filtering to obtain a clear solution;

[0064] (5) Add the auxiliary ligand solution obtained in step 3 to the clear solution obtained in step 4, stir for 0.5 h, and then filter to obtain a blue c...

Embodiment 2 Embodiment 1

[0072] Example 2 Detection of HIV Nucleic Acid Sequence Using Example 1 Copper Metal Coordination Polymer

[0073] (1) Determination of HIV-1 nucleic acid sequence to be detected

[0074]Through the joint search of GeneBank and other related databases, the ds DNA sequence with a size of 20-30bp and 100% specificity in the conserved sequence of HIV-1 is screened out, artificially synthesized as the sequence to be detected, that is, target DNA, and complemented The strand cDNA sequence is fluorescently labeled (P-DNA), and the sequence is as follows:

[0075] target 1 DNA sequence (T 1 -DNA): 5'-AGAAAAAAGAAGAA-3'

[0076] P 1 - DNA sequence complementary strand (P 1 -DNA): 5'-FAM-TTCTTCTTTTTTCT-3'

[0077] (2) Preparation of copper metal coordination polymer aqueous solution

[0078] The copper metal coordination polymer was dissolved in distilled water to prepare a solution with a concentration of 250 mM, and the solution was sterilized with a sterile filter, and sealed ...

Embodiment 3 Embodiment 1

[0083] Example 3 Detection of Sudan Virus Nucleic Acid Sequence Using Copper Metal Coordination Polymer in Example 1

[0084] (1) Determination of the nucleic acid sequence of Sudan virus SUDV to be tested

[0085] Through the joint search of GeneBank and other related databases, the 20-30bp RNA sequence with 100% specificity in the Sudan virus SUDV conservative sequence was screened out, and it was artificially synthesized as the sequence to be detected, that is, target RNA. Considering the relative instability of RNA, the complementary strand of target RNA was replaced with its complementary DNA (cDNA), and the cDNA sequence was fluorescently labeled, namely probe DNA, the sequence is as follows:

[0086] target 2 RNA sequence (T 2 -RNA): 5'-GAUGAGGACAAACUUUUUAA-3'

[0087] P 2 - DNA sequence complementary strand (P 2 -DNA): 5'-FAM-TTAAAAAGTTTGTCCTCATC-3'

[0088] (2) Preparation of copper metal coordination polymer aqueous solution

[0089] The copper metal coordinat...

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Abstract

The invention relates to an amphoteric carboxylic acid three-dimensional metal coordination polymer, and a preparation method and application thereof. The invention specifically discloses a water-soluble amphoteric carboxylic acid three-dimensional metal coordination polymer, wherein the main ligand of the three-dimensional copper coordination polymer is N-(3,5-dicarboxylbenzyl)-(3-carboxyl)-pyridine bromide; the auxiliary ligand of the polymer is 1,2-bis(4-pyridyl)ethylene; and central atom of the polymer is copper. According to the invention, raw materials are widely and easily available; the preparation method is simple in process, mild in reaction conditions and good in repeatability; and the amphoteric carboxylic acid three-dimensional metal copper coordination polymer has good water-solubility, can be successfully applied to detection of nucleic acid sequences of AIDS, SUDV, dengue viruses and the like, has the advantages of rapidness, convenience, high efficiency and sensitivity, and shows good application prospects in detection of related diseases.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a preparation method and application of an amphoteric carboxylic acid three-dimensional copper metal coordination polymer. Background technique [0002] In recent years, the application of fluorescent probes to detect nucleic acid molecules based on the principle of fluorescence spectroscopy has become a hot spot for scientific researchers. This method has the advantages of sensitivity, rapidity, simplicity and low cost. According to literature reports, carbon nanomaterials, nanometals, and some metal coordination polymers can be used as fluorescent probes for nucleic acid detection. However, materials such as carbon nanomaterials and nanometals have disadvantages such as complicated synthesis, high production costs, and poor solubility in water, which limit the application research of these materials in nucleic acid detection. [0003] Metal coordination polymers are a new t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00G01N21/64
CPCC08G83/008G01N21/6428G01N21/6486G01N2021/6432
Inventor 谢宝平陈金香孙斌杨水平邱桂华张茉莉
Owner SOUTHERN MEDICAL UNIVERSITY
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