Amphoteric carboxylic acid three-dimensional metal coordination polymer, and preparation method and application thereof
A technology of coordination polymer and amphoteric carboxylic acid, applied in instruments, analytical materials, measuring devices, etc., can solve the problems of collapse of pore structure, poor water solubility, hindering the practical application research of nucleic acid molecule detection and so on.
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Embodiment 1
[0058] Embodiment 1 prepares metal coordination polymer
[0059] 1. Preparation of amphoteric carboxylic acid three-dimensional copper metal coordination polymer
[0060] (1) Disperse N-(3,5-dicarboxybenzyl)-(3-carboxy)-pyridine bromide (0.764g, 2mmol) in distilled water (70mL), adjust the pH to 7.0 with 0.1M NaOH solution, Obtain the main ligand solution;
[0061] (2) CuSO 4 ·5H 2 O (0.391g, 2mmol) was dissolved in distilled water (20mL) to obtain a copper salt solution;
[0062] (3) 1,2-bis(4-pyridyl)ethylene (0.360g, 2mmol) was dissolved in DMF solution (5mL) to obtain an auxiliary ligand solution;
[0063] (4) adding the copper salt solution obtained in step 2 dropwise to the main ligand solution obtained in step 1, stirring and reacting at room temperature for 0.5h and then filtering to obtain a clear solution;
[0064] (5) Add the auxiliary ligand solution obtained in step 3 to the clear solution obtained in step 4, stir for 0.5 h, and then filter to obtain a blue c...
Embodiment 2 Embodiment 1
[0072] Example 2 Detection of HIV Nucleic Acid Sequence Using Example 1 Copper Metal Coordination Polymer
[0073] (1) Determination of HIV-1 nucleic acid sequence to be detected
[0074]Through the joint search of GeneBank and other related databases, the ds DNA sequence with a size of 20-30bp and 100% specificity in the conserved sequence of HIV-1 is screened out, artificially synthesized as the sequence to be detected, that is, target DNA, and complemented The strand cDNA sequence is fluorescently labeled (P-DNA), and the sequence is as follows:
[0075] target 1 DNA sequence (T 1 -DNA): 5'-AGAAAAAAGAAGAA-3'
[0076] P 1 - DNA sequence complementary strand (P 1 -DNA): 5'-FAM-TTCTTCTTTTTTCT-3'
[0077] (2) Preparation of copper metal coordination polymer aqueous solution
[0078] The copper metal coordination polymer was dissolved in distilled water to prepare a solution with a concentration of 250 mM, and the solution was sterilized with a sterile filter, and sealed ...
Embodiment 3 Embodiment 1
[0083] Example 3 Detection of Sudan Virus Nucleic Acid Sequence Using Copper Metal Coordination Polymer in Example 1
[0084] (1) Determination of the nucleic acid sequence of Sudan virus SUDV to be tested
[0085] Through the joint search of GeneBank and other related databases, the 20-30bp RNA sequence with 100% specificity in the Sudan virus SUDV conservative sequence was screened out, and it was artificially synthesized as the sequence to be detected, that is, target RNA. Considering the relative instability of RNA, the complementary strand of target RNA was replaced with its complementary DNA (cDNA), and the cDNA sequence was fluorescently labeled, namely probe DNA, the sequence is as follows:
[0086] target 2 RNA sequence (T 2 -RNA): 5'-GAUGAGGACAAACUUUUUAA-3'
[0087] P 2 - DNA sequence complementary strand (P 2 -DNA): 5'-FAM-TTAAAAAGTTTGTCCTCATC-3'
[0088] (2) Preparation of copper metal coordination polymer aqueous solution
[0089] The copper metal coordinat...
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