Detection method of fructus aurantii medicine material fingerprint and standard fingerprint

Abstract

The invention discloses a detection method of a fructus aurantii medicine material fingerprint and a standard fingerprint, and application of the fructus aurantii medicine material fingerprint or the standard fingerprint in identifying a fructus aurantii medicine material. The method includes the steps that firstly, the fructus aurantii medicine material is obtained and smashed into coarse powder and precisely weighed, methyl alcohol is added, extraction is conducted, a subsequent filtrate obtained, and a test sample solution is obtained; secondly, a reference substance solution is prepared; thirdly, the test sample solution and the reference substance solution are precisely sucked and injected into a high performance liquid chromatograph respectively to be measured, and the fructus aurantii fingerprint is obtained. The measurement method is easy and convenient to operate, characteristic components are maintained completely, repeatability and stability are good, precision is high, specificity is high, and the method plays a positive role in guiding production charging and guaranteeing quality reliability. The fructus aurantii medicine material fingerprint built through the method can be effectively used for monitoring the quality of the fructus aurantii medicine material and identifying whether the fructus aurantii medicine material is true or fake.

Description

technical field [0001] The invention belongs to the technical field of medicine analysis, and in particular relates to a fingerprint of a Fructus Fructus medicinal material and a method for establishing the same. Specifically, the present invention relates to a high-performance liquid chromatography (HPLC) switching wavelength fingerprint and its establishment method of the medicinal material Fructus aurantii, and a standard fingerprint of Fructus aurantii. The invention also relates to a method for identifying the quality of the Fructus Fructus medicinal material. Background technique [0002] Citrus aurantium is the dry immature fruit of Citrus aurantium L and its cultivars of the Rutaceae citrus plant. The cultivars recorded in the 2015 edition of "Chinese Pharmacopoeia" are Citrus aurantium 'Huangpi', Citms aurantium 'Tangcheng', Citrus aurantium 'Chuluan', and Daidaihua (Citrus aurantium 'Tangcheng'). 'Daidai'), the dried immature fruit of Citrus aurantium is harveste...

AI Technical Summary

Problems solved by technology

[0003] The 2015 edition of the Pharmacopoeia stipulates that naringin and neohesperidin are used as indicators for quality control of Citrus aurantii (Compiled by the National Pharmacopoeia Committee, Pharmacopoeia of the People's Republic of China 2015 Edition, Beijing: China Pharmaceutical Industry Press, 2015, Part One: 246), The method of using two index components to control the quality of traditional Chinese medicine cannot fully reflect the type and quantity of the internal chemical components of traditional Chinese medicine, and has certain limitations, and cannot fully control the quality of medicinal materials

"Straits Pharmacy" 2003 No. 6 "Research on the Fingerprint of Fructus Aurantii Lvyi" recorded a method of using gas chromatography (GC) to measure the fingerprints of volatile oils in Fructus aurantium Lvyi; "Chinese Journal of Pharmaceutical Sciences" 2005 No. 11 The issue "Research on HPLC Fingerprint Analysis and Chemical Pattern Recognition Classification of Accumulated Shells of Traditional Chinese Medicine" recorded the application of high performance liquid chromatography (HPLC) to determine the fingerprint method of Citrus aurantii, but there were only 8 common peaks and only naringin and hesperidin were identified. , neohesperidin and other three index components; "Chinese Herbal Medicine" 2009 No. 3 "Research on HPLC Fingerprints of Different Cultivated Fructus Citrus Fructus" recorded the method for determining the HPLC fingerprints of Citrus aurantium, but only using a single wavelength, there is no common peaks to identify the index components; "Chinese Journal of Traditional Chinese Medicine" 20th issue "Research on Capillary Electrophoresis Fingerprints of Fructus Fructus Medicinal Materials of Different Origins and Varieties" in 2008 recorded the fingerprint method for the determination of Fructus Fructus by capillary electrophoresis; "Modern Chinese Medicine" 2009 The 12th issue of 2010 "Research on HPLC Fingerprints of Bran Stir-fried Shells" recorded the method of using high-performance liquid chromatography to determine the fingerprints of processed products of Citrus aurantii, but only using a single wavelength to measure, and only identified naringin and neohesperidin. , hesperidin 3 characteristic peaks; "Chinese Modern Applied Pharmacy" 2015 No. 4 "Quality Investigation and Fingerprint Research of Citrus Fructus Decoction Pieces Commercially Available" records the application of high performance liquid chromatography to determine the fingerprint method of Fructus Fructus decoction pieces, Using a single wavelength with only 5 common peaks and only identifying four index components such as rutin naringin, naringin, hesperidin and neohesperidin

In the prior art, there are few index components for identification, which cannot fully reflect the quality of medicinal materials and ensure the curative effect of medication. Therefore, it is of great significance to establish a fingerprint detection method and a standard fingerprint method for medicinal materials of Fructus Aurantii

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