Detection method of fructus aurantii medicine material fingerprint and standard fingerprint

A technology of fingerprint spectrum and detection method, applied in the field of drug analysis, can solve the problems that cannot fully reflect the type and quantity of internal chemical components of traditional Chinese medicine, cannot fully reflect the quality of medicinal materials, and cannot fully control the quality of medicinal materials, etc., and achieve a perfect quality evaluation system. , Enhance the effect of quality monitoring and guiding feeding

Inactive Publication Date: 2017-03-22
HUNAN HANSEN PHARMACEUTICAL CO LTD
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AI Technical Summary

Problems solved by technology

[0003] The 2015 edition of the Pharmacopoeia stipulates that naringin and neohesperidin are used as indicators for quality control of Citrus aurantii (Compiled by the National Pharmacopoeia Committee, Pharmacopoeia of the People's Republic of China 2015 Edition, Beijing: China Pharmaceutical Industry Press, 2015, Part One: 246), The method of using two index components to control the quality of traditional Chinese medicine cannot fully reflect the type and quantity of the internal chemical components of traditional Chinese medicine, and has certain limitations, and cannot fully control the quality of medicinal materials
"Straits Pharmacy" 2003 No. 6 "Research on the Fingerprint of Fructus Aurantii Lvyi" recorded a method of using gas chromatography (GC) to measure the fingerprints of volatile oils in Fructus aurantium Lvyi; "Chinese Journal of Pharmaceutical Sciences" 2005 No. 11 The issue "Research on HPLC Fingerprint Analysis and Chemical Pattern Recognition Classification of Accumulated Shells of Traditional Chinese Medicine" recorded the application of high performance liquid chromatography (HPLC) to determine the fingerprint method of Citrus aurantii, but there were only 8 common peaks and only naringin and hesperidin were identified. , neohesperidin and other three index components; "Chinese Herbal Medicine" 2009 No. 3 "Research on HPLC Fingerprints of Different Cultivated Fructus Citrus Fructus" recorded the method for determining the HPLC fingerprints of Citrus aurantium, but only using a single wavel

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  • Detection method of fructus aurantii medicine material fingerprint and standard fingerprint
  • Detection method of fructus aurantii medicine material fingerprint and standard fingerprint
  • Detection method of fructus aurantii medicine material fingerprint and standard fingerprint

Examples

Experimental program
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Example Embodiment

[0045] Example 1: Establishment and verification of the switching wavelength fingerprint of Fructus Aurantii.

[0046] 1.1 Instruments and reagents:

[0047] Agilent 1260 high performance liquid chromatograph, including online vacuum degassing machine (G-1311C), binary pump (G-1311C), standard autosampler (G-1329B), and intelligent column oven (G-1316A) , DAD detector (G-1314B), Agilent1260 Infinity chromatography workstation (Agilent Technologies, USA); SB-5200D ultrasonic cleaner (Ningbo Xinzhi Biotechnology Co., Ltd.); FAZ004B analytical balance (Shanghai Youke); BT125D electronics Balance (Sartorius Scientific Instruments (Beijing) Co., Ltd.).

[0048] 1.2 Selection of chromatographic conditions.

[0049] (1) Selection of mobile phase

[0050] Choose acetonitrile-0.01% phosphoric acid solution, acetonitrile-0.1% phosphoric acid solution, acetonitrile-4% phosphoric acid solution, acetonitrile-0.01% formic acid solution, acetonitrile-0.1% formic acid solution, acetonitrile-4% formic...

Example Embodiment

[0084] Example 2: The use of standard fingerprints in the identification of Citrus aurantium medicinal materials.

[0085] 1.1 Instruments and reagents:

[0086] Agilent 1260 high performance liquid chromatograph, including online vacuum degassing machine (G-1311C), binary pump (G-1311C), standard autosampler (G-1329B), and intelligent column oven (G-1316A) , DAD detector (G-1314B), Agilent1260 Infinity chromatography workstation (Agilent Technologies, USA); SB-5200D ultrasonic cleaner (Ningbo Xinzhi Biotechnology Co., Ltd.); FAZ004B analytical balance (Shanghai Youke); BT125D electronics Balance (Sartorius Scientific Instruments (Beijing) Co., Ltd.).

[0087] 1.2 Determination of the HPLC fingerprint of Fructus Aurantii.

[0088] (1) Preparation of test solution

[0089] Separately take 0.2g of Fructus Aurantii crude powder, accurately weigh it, place it in a conical flask with a stopper, precision methanol 50ml, close stopper, weigh it, ultrasonically extract for 1h, let it cool, we...

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Abstract

The invention discloses a detection method of a fructus aurantii medicine material fingerprint and a standard fingerprint, and application of the fructus aurantii medicine material fingerprint or the standard fingerprint in identifying a fructus aurantii medicine material. The method includes the steps that firstly, the fructus aurantii medicine material is obtained and smashed into coarse powder and precisely weighed, methyl alcohol is added, extraction is conducted, a subsequent filtrate obtained, and a test sample solution is obtained; secondly, a reference substance solution is prepared; thirdly, the test sample solution and the reference substance solution are precisely sucked and injected into a high performance liquid chromatograph respectively to be measured, and the fructus aurantii fingerprint is obtained. The measurement method is easy and convenient to operate, characteristic components are maintained completely, repeatability and stability are good, precision is high, specificity is high, and the method plays a positive role in guiding production charging and guaranteeing quality reliability. The fructus aurantii medicine material fingerprint built through the method can be effectively used for monitoring the quality of the fructus aurantii medicine material and identifying whether the fructus aurantii medicine material is true or fake.

Description

technical field [0001] The invention belongs to the technical field of medicine analysis, and in particular relates to a fingerprint of a Fructus Fructus medicinal material and a method for establishing the same. Specifically, the present invention relates to a high-performance liquid chromatography (HPLC) switching wavelength fingerprint and its establishment method of the medicinal material Fructus aurantii, and a standard fingerprint of Fructus aurantii. The invention also relates to a method for identifying the quality of the Fructus Fructus medicinal material. Background technique [0002] Citrus aurantium is the dry immature fruit of Citrus aurantium L and its cultivars of the Rutaceae citrus plant. The cultivars recorded in the 2015 edition of "Chinese Pharmacopoeia" are Citrus aurantium 'Huangpi', Citms aurantium 'Tangcheng', Citrus aurantium 'Chuluan', and Daidaihua (Citrus aurantium 'Tangcheng'). 'Daidai'), the dried immature fruit of Citrus aurantium is harveste...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 刘令安杨华张妮瑜林俊华张建伟王雄龙郭元满
Owner HUNAN HANSEN PHARMACEUTICAL CO LTD
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