Detection method for fructus aurantii immaturus medicine finger-print, and standard finger-print

Abstract

The invention discloses a detection method for a fructus aurantii immaturus medicine finger-print, a standard finger-print thereof, and application of the fructus aurantii immaturus medicine finger-print or the standard finger-print in identifying fructus aurantii immaturus medicines. The method comprises the following steps: (1) taking the fructus aurantii immaturus medicines, smashing into medium-granularity powder, precisely weighting, adding methyl alcohol, carrying out extraction, and taking subsequent filtrate to obtain a solution of a sample for testing; (2) preparing reference substance solution; (3) respectively precisely absorbing the solution of the sample for testing and the reference substance solution, and injecting into a high performance liquid chromatograph to measure, so as to obtain the fructus aurantii immaturus finger-print. The measurement method disclosed by the invention has the advantages of convenience in operation, good repeatability and stability, high accuracy and high specificity, and has an active function on guiding production feeding and guaranteeing quality reliability, and characteristic ingredients are integrally kept. The fructus aurantii immaturus medicine finger-print established by the method can be used for effectively monitoring the quality of the fructus aurantii immaturus medicines and identifying fakes from originals.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a fingerprint of a citrus citrus medicinal material and a method for establishing the same. Specifically, the present invention relates to a high-performance liquid chromatography (HPLC) switching wavelength method fingerprint and its establishment method, as well as a standard fingerprint of Citrus aurantium medicinal material. The invention also relates to a method for identifying the quality of Citrus aurantii medicinal material. Background technique [0002] Citrus aurantium is the dried young fruit of Citrus aurantium L. and its cultivar C. Sinensis (L) Osbeck of Rutaceae, mainly produced in Jiangxi, Sichuan, Fujian, Jiangsu and other places. The 2015 edition of the "Chinese Pharmacopoeia" records that Citrus aurantium has the effect of dispelling qi and eliminating swelling, reducing phlegm and eliminating stagnation. It is used in modern clinical practic...

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Problems solved by technology

[0005] The 2015 edition of the Pharmacopoeia stipulates that synephrine is used as an index for the quality control of Citrus aurantium (edited by the National Pharmacopoeia Committee, the Pharmacopoeia of the People's Republic of China 2015 edition, Beijing: China Pharmaceutical Industry Press, 2015, one part: 247), and a single component is used for traditional Chinese medicine. The quality control method cannot fully reflect the types and quantities of the internal chemical components of traditional Chinese medicine, and has certain limitations, and cannot fully control the quality of medicinal materials

[0006] The research on Fructus Aurantii mainly involves extraction, processing, etc., and the quality detection methods are less publicized. For example, Chinese patent 200410077903.6 (publication number CN100486586C) involves the detection of total flavonoids in the Fructus Fructus Fructus extract, not on the medicinal materials of Fructus Fructus Fructus Aurantii. testing, and did not fully control the quality; Chinese patent 200710120306.0 (publication number CN101366849B) involves the use of infrared spectroscopy to control the quality of Citrus aurantium medicinal materials, and only detects three index components, which has certain limitations; Chinese patent 201410106425.0 (Publication No. CN 103837621B) relates to the HPLC fingerprint of the medicinal material Aurantium citrifolia, which only uses a single wavelength for detection, and the preparation method of the test sample of citrus aurantium is complicated

"Chinese Pharmacy" No. 39, 2011 "Studies on the HPLC Fingerprints of Two Kinds of Citrus Aurantium Medicinal Materials, Sour Orange and Sweet Orange, and Analysis of the Contents of Naringin and Synephrine" records a method for the determination of Citrus aurantium by high performance liquid chromatography (HPLC). fingerprint method, but only identified four index components such as naringin, hesperidin, neohesperidin, and synephrine; A method for determining the fingerprint of volatile oil in Aurantium citrus Lvyi by gas chromatography (GC); "Modern Chinese Medicine Research and Practice" in 2004 Volume 18 Supplement "Application of Chemical Pattern Recognition in the Classification of Accumulation of Traditional Chinese Medicine" recorded a A method using high performance liquid chromatography (HPLC) to determine the fingerprints of Citrus aurantium, but using a single wavelength detection, there are only 8 common peaks and only three index components such as naringin, hesperidin and neohesperidin are identified

In the prior art, there are few index components for identification, which cannot fully reflect the quality of medicinal materials and ensure the curative effect of medication. Therefore, it is of great significance to establish a fingerprint detection method and a standard fingerprint method for medicinal materials of Citrus aurantium

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