Fusarium oxysporum fungicide and application of citral
A Fusarium oxysporum fungicide technology, applied in the application field of Fusarium oxysporum fungicide and citral, can solve the problems of slow growth, affecting the yield and quality of Chinese medicinal materials, and insufficient nutrients
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Embodiment 1-8
[0019] Make citral in water to a concentration of 4, 6, 8, 10g·L -1 The dispersion liquid, respectively, to obtain 4g·L -1 Citral dispersion (fungicide 1), 6g L -1 Citral dispersion (fungicide 2), 8g L -1 Citral dispersion (fungicide 3), 10g L -1 Citral dispersion (fungicide 4).
[0020] The Fusarium oxysporum used was preserved by the Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences. The fungicide 1-4 was used to test the antibacterial effect of Fusarium oxysporum, and the effect of different concentrations of citral solutions on Fusarium oxysporum was verified.
[0021] Specifically, take 20 mL of sterilized potato dextrose agar (PDA) medium liquid and add it to a sterile petri dish, and draw 400 μL of 1×10 8 cfu ml -1 The Fusarium oxysporum suspension was evenly spread on the solidified PDA, and four sterilized filter paper discs with a diameter of 8mm were evenly glued to the petri dish. see figure 1 , A represents the sterilized filte...
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